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Application of asymmetric flow field-flow fractionation (AF4) and multiangle light scattering (MALS) for the evaluation of changes in the product molar mass during PVP-b-PAMPS synthesis

Fuentes, Catalina LU ; Castillo, Joel; Vila, Jose LU and Nilsson, Lars LU (2018) In Analytical and Bioanalytical Chemistry 410(16). p.3757-3767
Abstract

The use of polymers for the delivery of drugs has increased dramatically in the last decade. To ensure the desired properties and functionality of such substances, adequate characterization in terms of the molar mass (M) and size is essential. The aim of this study was to evaluate the changes in the M and size of PVP-b-PAMPS when the amounts of the synthesis reactants in the two-step radical reaction were varied. The determination of the M and size distributions was performed by an asymmetric flow field-flow fractionation (AF4) system connected to multiangle light scattering (MALS) and differential refractive index (dRI) detectors. The results show that the M of the polymers varies depending on the relative amounts of the reactants and... (More)

The use of polymers for the delivery of drugs has increased dramatically in the last decade. To ensure the desired properties and functionality of such substances, adequate characterization in terms of the molar mass (M) and size is essential. The aim of this study was to evaluate the changes in the M and size of PVP-b-PAMPS when the amounts of the synthesis reactants in the two-step radical reaction were varied. The determination of the M and size distributions was performed by an asymmetric flow field-flow fractionation (AF4) system connected to multiangle light scattering (MALS) and differential refractive index (dRI) detectors. The results show that the M of the polymers varies depending on the relative amounts of the reactants and that AF4-MALS-dRI is a powerful characterization technique for analyzing polymers. Using AF4, it was possible to separate the product of the first radical reaction (PVP-CTA) into two populations. The first population had an elongated, rod-like or random coil conformation, and the second had a conformation corresponding to homogeneous spheres or a microgel structure. PVP-b-PAMPS had only one population, which had a rod-like conformation. The molar masses of PVP-CTA and PVP-b-PAMPS found in this study were higher than those reported in previous studies.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Asymmetric flow field-flow fractionation (AF4), Changes in molar mass, PVP-b-PAMPS, Synthesis
in
Analytical and Bioanalytical Chemistry
volume
410
issue
16
pages
3757 - 3767
publisher
Springer
external identifiers
  • scopus:85045480999
ISSN
1618-2642
DOI
10.1007/s00216-018-1039-1
language
English
LU publication?
yes
id
40e454d7-2d61-4e22-8a6a-b203dabd5660
date added to LUP
2018-04-26 15:03:49
date last changed
2019-01-14 15:40:47
@article{40e454d7-2d61-4e22-8a6a-b203dabd5660,
  abstract     = {<p>The use of polymers for the delivery of drugs has increased dramatically in the last decade. To ensure the desired properties and functionality of such substances, adequate characterization in terms of the molar mass (M) and size is essential. The aim of this study was to evaluate the changes in the M and size of PVP-b-PAMPS when the amounts of the synthesis reactants in the two-step radical reaction were varied. The determination of the M and size distributions was performed by an asymmetric flow field-flow fractionation (AF4) system connected to multiangle light scattering (MALS) and differential refractive index (dRI) detectors. The results show that the M of the polymers varies depending on the relative amounts of the reactants and that AF4-MALS-dRI is a powerful characterization technique for analyzing polymers. Using AF4, it was possible to separate the product of the first radical reaction (PVP-CTA) into two populations. The first population had an elongated, rod-like or random coil conformation, and the second had a conformation corresponding to homogeneous spheres or a microgel structure. PVP-b-PAMPS had only one population, which had a rod-like conformation. The molar masses of PVP-CTA and PVP-b-PAMPS found in this study were higher than those reported in previous studies.</p>},
  author       = {Fuentes, Catalina and Castillo, Joel and Vila, Jose and Nilsson, Lars},
  issn         = {1618-2642},
  keyword      = {Asymmetric flow field-flow fractionation (AF4),Changes in molar mass,PVP-b-PAMPS,Synthesis},
  language     = {eng},
  month        = {04},
  number       = {16},
  pages        = {3757--3767},
  publisher    = {Springer},
  series       = {Analytical and Bioanalytical Chemistry},
  title        = {Application of asymmetric flow field-flow fractionation (AF4) and multiangle light scattering (MALS) for the evaluation of changes in the product molar mass during PVP-b-PAMPS synthesis},
  url          = {http://dx.doi.org/10.1007/s00216-018-1039-1},
  volume       = {410},
  year         = {2018},
}