Time and concentration dependence of Fenton-induced oxidation of dG
(2006) In Nucleosides, Nucleotides & Nucleic Acids 25(3). p.259-278- Abstract
- The influence of incubation time and Fenton reagent concentrations was investigated on the oxidation of 2'-deoxyguanosine. The compounds identified and quantified, through use of an LC-MS/MS system, were 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8,5'- cyclo-2'-deoxyguanosine (8,5'cyclodG) and the secondary oxidation products guanidinohydantoin and dehydro-guanidinohydantoin. 8-oxodG and 8,5'cyclodG formed very quickly, reaching a maximum rapidly, but with 8-oxodC a rapid decline occurred thereafter due to its further oxidation into the secondary products, which formed more slowly. Due to the better stability, 8,5'cyclodG correlated better with the general level of oxidation than 8-oxodG. The results emphasize the advantages of... (More)
- The influence of incubation time and Fenton reagent concentrations was investigated on the oxidation of 2'-deoxyguanosine. The compounds identified and quantified, through use of an LC-MS/MS system, were 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8,5'- cyclo-2'-deoxyguanosine (8,5'cyclodG) and the secondary oxidation products guanidinohydantoin and dehydro-guanidinohydantoin. 8-oxodG and 8,5'cyclodG formed very quickly, reaching a maximum rapidly, but with 8-oxodC a rapid decline occurred thereafter due to its further oxidation into the secondary products, which formed more slowly. Due to the better stability, 8,5'cyclodG correlated better with the general level of oxidation than 8-oxodG. The results emphasize the advantages of measuring other oxidation adducts than 8-oxodG atone. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/413934
- author
- Grey, Carl LU and Adlercreutz, Patrick LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Fenton reaction, hydrogen peroxide, iron sulfate, 8-oxo-7, 8-dihydro-2 '-deoxyguanosine, LC-MS/MS, 8, 5 '-cyclo-2, '-deoxyguanosine, guanidinohydantoin, dehydro-guanidinohydantoin, free radicals, quantification, of modified nucleosides, hydroxyl radical, modified nucleosides, oxidative damage, 2 '-deoxyguanosine
- in
- Nucleosides, Nucleotides & Nucleic Acids
- volume
- 25
- issue
- 3
- pages
- 259 - 278
- publisher
- Taylor & Francis
- external identifiers
-
- wos:000236794200003
- scopus:33646429061
- ISSN
- 1525-7770
- DOI
- 10.1080/15257770500446956
- language
- English
- LU publication?
- yes
- id
- a52b51c1-a713-4807-a17c-8be33b594560 (old id 413934)
- date added to LUP
- 2016-04-01 12:16:06
- date last changed
- 2022-01-27 01:17:27
@article{a52b51c1-a713-4807-a17c-8be33b594560, abstract = {{The influence of incubation time and Fenton reagent concentrations was investigated on the oxidation of 2'-deoxyguanosine. The compounds identified and quantified, through use of an LC-MS/MS system, were 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8,5'- cyclo-2'-deoxyguanosine (8,5'cyclodG) and the secondary oxidation products guanidinohydantoin and dehydro-guanidinohydantoin. 8-oxodG and 8,5'cyclodG formed very quickly, reaching a maximum rapidly, but with 8-oxodC a rapid decline occurred thereafter due to its further oxidation into the secondary products, which formed more slowly. Due to the better stability, 8,5'cyclodG correlated better with the general level of oxidation than 8-oxodG. The results emphasize the advantages of measuring other oxidation adducts than 8-oxodG atone.}}, author = {{Grey, Carl and Adlercreutz, Patrick}}, issn = {{1525-7770}}, keywords = {{Fenton reaction; hydrogen peroxide; iron sulfate; 8-oxo-7; 8-dihydro-2 '-deoxyguanosine; LC-MS/MS; 8; 5 '-cyclo-2; '-deoxyguanosine; guanidinohydantoin; dehydro-guanidinohydantoin; free radicals; quantification; of modified nucleosides; hydroxyl radical; modified nucleosides; oxidative damage; 2 '-deoxyguanosine}}, language = {{eng}}, number = {{3}}, pages = {{259--278}}, publisher = {{Taylor & Francis}}, series = {{Nucleosides, Nucleotides & Nucleic Acids}}, title = {{Time and concentration dependence of Fenton-induced oxidation of dG}}, url = {{http://dx.doi.org/10.1080/15257770500446956}}, doi = {{10.1080/15257770500446956}}, volume = {{25}}, year = {{2006}}, }