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Different patterns in immunoreactive anionic and cationic trypsinogen in urine and serum in human acute pancreatitis

Petersson, Ulf LU ; Appelros, Stefan LU and Borgström, Anders LU (1999) In International Journal of Pancreatology 25(3). p.165-170
Abstract
BACKGROUND:



Acute pancreatitis (AP) results in elevated concentrations of trypsinogen (T) isoenzymes in serum. Immunoreactive anionic trypsinogen in urin (irAT/u) is elevated in AP, and has recently been proposed as a rapid diagnostic instrument and severity predictor. These results have not been confirmed by other groups, and irAT/u has not been further characterized. The concentration of immunoreactive cationic trypsinogen in urine (irCT/u) and the serum irAT/irCT ratio in AP have not been extensively examined.



METHODS:



Levels of irAT and irCT were studied in urine and serum from 50 AP patients and in urine from 41 non-AP patients. Severity was assessed according to the Atlanta... (More)
BACKGROUND:



Acute pancreatitis (AP) results in elevated concentrations of trypsinogen (T) isoenzymes in serum. Immunoreactive anionic trypsinogen in urin (irAT/u) is elevated in AP, and has recently been proposed as a rapid diagnostic instrument and severity predictor. These results have not been confirmed by other groups, and irAT/u has not been further characterized. The concentration of immunoreactive cationic trypsinogen in urine (irCT/u) and the serum irAT/irCT ratio in AP have not been extensively examined.



METHODS:



Levels of irAT and irCT were studied in urine and serum from 50 AP patients and in urine from 41 non-AP patients. Severity was assessed according to the Atlanta classification. irAT/u was characterized by gel filtration.



RESULTS:



Gel filtration revealed only AT in the urine. Highly significant differences in irAT/u were seen between AP/non-AP (p < 0.0001) and mild/severe disease (p = 0.0012). The irAT/irCT ratio in serum changed from normal 0.8 to 1.3 in AP.



CONCLUSIONS:



IrAT and only traces of irCT were found in the urine in AP. IrAT/u was higher in AP than in other acute abdominal disorders (non-AP) and also higher in severe than in mild AP. IrAT in serum (irAT/s) increased proportionally more than irCT/s in AP, but did not discriminate mild from severe forms. High levels of irAT/u in some non-AP cases and a wide range in AP cases make the clinical value of the test questionable. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Journal of Pancreatology
volume
25
issue
3
pages
165 - 170
publisher
Humana Press
external identifiers
  • scopus:0032800331
ISSN
0169-4197
language
English
LU publication?
yes
id
a48d9f9f-76bc-4e42-b515-747b65182060 (old id 4139585)
alternative location
http://link.springer.com/article/10.1007/BF02925965
date added to LUP
2016-01-25 10:27:55
date last changed
2017-01-01 07:13:42
@article{a48d9f9f-76bc-4e42-b515-747b65182060,
  abstract     = {BACKGROUND: <br/><br>
<br/><br>
Acute pancreatitis (AP) results in elevated concentrations of trypsinogen (T) isoenzymes in serum. Immunoreactive anionic trypsinogen in urin (irAT/u) is elevated in AP, and has recently been proposed as a rapid diagnostic instrument and severity predictor. These results have not been confirmed by other groups, and irAT/u has not been further characterized. The concentration of immunoreactive cationic trypsinogen in urine (irCT/u) and the serum irAT/irCT ratio in AP have not been extensively examined.<br/><br>
<br/><br>
METHODS: <br/><br>
<br/><br>
Levels of irAT and irCT were studied in urine and serum from 50 AP patients and in urine from 41 non-AP patients. Severity was assessed according to the Atlanta classification. irAT/u was characterized by gel filtration.<br/><br>
<br/><br>
RESULTS: <br/><br>
<br/><br>
Gel filtration revealed only AT in the urine. Highly significant differences in irAT/u were seen between AP/non-AP (p &lt; 0.0001) and mild/severe disease (p = 0.0012). The irAT/irCT ratio in serum changed from normal 0.8 to 1.3 in AP.<br/><br>
<br/><br>
CONCLUSIONS: <br/><br>
<br/><br>
IrAT and only traces of irCT were found in the urine in AP. IrAT/u was higher in AP than in other acute abdominal disorders (non-AP) and also higher in severe than in mild AP. IrAT in serum (irAT/s) increased proportionally more than irCT/s in AP, but did not discriminate mild from severe forms. High levels of irAT/u in some non-AP cases and a wide range in AP cases make the clinical value of the test questionable.},
  author       = {Petersson, Ulf and Appelros, Stefan and Borgström, Anders},
  issn         = {0169-4197},
  language     = {eng},
  number       = {3},
  pages        = {165--170},
  publisher    = {Humana Press},
  series       = {International Journal of Pancreatology},
  title        = {Different patterns in immunoreactive anionic and cationic trypsinogen in urine and serum in human acute pancreatitis},
  volume       = {25},
  year         = {1999},
}