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Influence of CrgA on assembly of the cell division protein FtsZ during development of Streptomyces coelicolor

Del Sol, R ; Mullins, JGL ; Grantcharova, Nina ; Flärdh, Klas LU and Dyson, P (2006) In Journal of Bacteriology 188(4). p.1540-1550
Abstract
The product of the crgA gene of Streptomyces coelicolor represents a novel family of small proteins. A single orthologous gene is located close to the origin of replication of all fully sequenced actinomycete genomes and borders a conserved gene cluster implicated in cell growth and division. In S. coelicolor, CrgA is important for coordinating growth and cell division in sporogenic hyphae. In this study, we demonstrate that CrgA is an integral membrane protein whose peak expression is coordinated with the onset of development of aerial hyphae. The protein localizes to discrete foci away from growing hyphal tips. Upon overexpression, CrgA localizes to apical syncytial cells of aerial hyphae and inhibits the formation of productive... (More)
The product of the crgA gene of Streptomyces coelicolor represents a novel family of small proteins. A single orthologous gene is located close to the origin of replication of all fully sequenced actinomycete genomes and borders a conserved gene cluster implicated in cell growth and division. In S. coelicolor, CrgA is important for coordinating growth and cell division in sporogenic hyphae. In this study, we demonstrate that CrgA is an integral membrane protein whose peak expression is coordinated with the onset of development of aerial hyphae. The protein localizes to discrete foci away from growing hyphal tips. Upon overexpression, CrgA localizes to apical syncytial cells of aerial hyphae and inhibits the formation of productive cytokinetic rings of the bacterial tubulin homolog FtsZ, leading to proteolytic turnover of this major cell division determinant. In the absence of known prokaryotic cell division inhibitors in actinomycetes, CrgA may have an important conserved function influencing Z-ring formation in these bacteria. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Bacteriology
volume
188
issue
4
pages
1540 - 1550
publisher
American Society for Microbiology
external identifiers
  • wos:000235407900038
  • pmid:16452438
  • scopus:32444446576
ISSN
0021-9193
DOI
10.1128/JB.188.4.1540-1550.2006
language
English
LU publication?
yes
id
5a258268-a399-4b5a-8640-0a2fa0e1fcc9 (old id 417415)
date added to LUP
2016-04-01 12:19:32
date last changed
2021-01-28 03:36:39
@article{5a258268-a399-4b5a-8640-0a2fa0e1fcc9,
  abstract     = {The product of the crgA gene of Streptomyces coelicolor represents a novel family of small proteins. A single orthologous gene is located close to the origin of replication of all fully sequenced actinomycete genomes and borders a conserved gene cluster implicated in cell growth and division. In S. coelicolor, CrgA is important for coordinating growth and cell division in sporogenic hyphae. In this study, we demonstrate that CrgA is an integral membrane protein whose peak expression is coordinated with the onset of development of aerial hyphae. The protein localizes to discrete foci away from growing hyphal tips. Upon overexpression, CrgA localizes to apical syncytial cells of aerial hyphae and inhibits the formation of productive cytokinetic rings of the bacterial tubulin homolog FtsZ, leading to proteolytic turnover of this major cell division determinant. In the absence of known prokaryotic cell division inhibitors in actinomycetes, CrgA may have an important conserved function influencing Z-ring formation in these bacteria.},
  author       = {Del Sol, R and Mullins, JGL and Grantcharova, Nina and Flärdh, Klas and Dyson, P},
  issn         = {0021-9193},
  language     = {eng},
  number       = {4},
  pages        = {1540--1550},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Bacteriology},
  title        = {Influence of CrgA on assembly of the cell division protein FtsZ during development of Streptomyces coelicolor},
  url          = {http://dx.doi.org/10.1128/JB.188.4.1540-1550.2006},
  doi          = {10.1128/JB.188.4.1540-1550.2006},
  volume       = {188},
  year         = {2006},
}