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Surface proteins of the Group G Streptococcus in different phases of growth: patterns of production and implications for the host-bacteria relationship.

Wollein Waldetoft, Kristofer LU ; Karlsson, Christofer LU ; Gram, Magnus LU ; Malmström, Johan LU ; Mörgelin, Matthias LU ; Frick, Inga-Maria LU and Björck, Lars LU (2014) In Microbiology 160(Nov 12). p.279-286
Abstract
Group G streptococcus (GGS) is a human bacterial pathogen expressing surface proteins FOG and protein G (PG) which interact with several host defense systems, including the complement and contact systems. Selected reaction monitoring mass spectrometry, electron microscopy, and protein binding assays were used to follow the amounts of FOG and PG intracellularly and on the bacterial surface during different phases of growth. Large and increasing amounts of PG were present on the surface in the stationary growth phase, and this was due to de novo production. In contrast, the amount of FOG did not change substantially during this phase. Apart from PG, a number of housekeeping proteins also increased in abundance in the stationary phase. These... (More)
Group G streptococcus (GGS) is a human bacterial pathogen expressing surface proteins FOG and protein G (PG) which interact with several host defense systems, including the complement and contact systems. Selected reaction monitoring mass spectrometry, electron microscopy, and protein binding assays were used to follow the amounts of FOG and PG intracellularly and on the bacterial surface during different phases of growth. Large and increasing amounts of PG were present on the surface in the stationary growth phase, and this was due to de novo production. In contrast, the amount of FOG did not change substantially during this phase. Apart from PG, a number of housekeeping proteins also increased in abundance in the stationary phase. These results show that GGS protein production is active during the stationary phase and that the bacteria actively remodel their surface and enter a less pro-inflammatory state in this phase. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Microbiology
volume
160
issue
Nov 12
pages
279 - 286
publisher
MAIK Nauka/Interperiodica
external identifiers
  • pmid:24222616
  • wos:000338603100004
  • scopus:84893505203
ISSN
1465-2080
DOI
10.1099/mic.0.071332-0
language
English
LU publication?
yes
id
8fbd6ec7-ed76-4e49-ace8-5b63af839d1e (old id 4179479)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24222616?dopt=Abstract
date added to LUP
2013-12-03 20:29:57
date last changed
2017-01-01 03:47:47
@article{8fbd6ec7-ed76-4e49-ace8-5b63af839d1e,
  abstract     = {Group G streptococcus (GGS) is a human bacterial pathogen expressing surface proteins FOG and protein G (PG) which interact with several host defense systems, including the complement and contact systems. Selected reaction monitoring mass spectrometry, electron microscopy, and protein binding assays were used to follow the amounts of FOG and PG intracellularly and on the bacterial surface during different phases of growth. Large and increasing amounts of PG were present on the surface in the stationary growth phase, and this was due to de novo production. In contrast, the amount of FOG did not change substantially during this phase. Apart from PG, a number of housekeeping proteins also increased in abundance in the stationary phase. These results show that GGS protein production is active during the stationary phase and that the bacteria actively remodel their surface and enter a less pro-inflammatory state in this phase.},
  author       = {Wollein Waldetoft, Kristofer and Karlsson, Christofer and Gram, Magnus and Malmström, Johan and Mörgelin, Matthias and Frick, Inga-Maria and Björck, Lars},
  issn         = {1465-2080},
  language     = {eng},
  number       = {Nov 12},
  pages        = {279--286},
  publisher    = {MAIK Nauka/Interperiodica},
  series       = {Microbiology},
  title        = {Surface proteins of the Group G Streptococcus in different phases of growth: patterns of production and implications for the host-bacteria relationship.},
  url          = {http://dx.doi.org/10.1099/mic.0.071332-0},
  volume       = {160},
  year         = {2014},
}