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Evidence for a morphologically distinct and functionally robust cell type in the proximal tubules of human kidney.

Hansson, Jennifer LU ; Hultenby, Kjell; Cramnert, Catharina; Pontén, Fredrik; Jansson, Hannes LU ; Lindgren, David LU ; Axelson, Håkan LU and Johansson, Martin LU (2014) In Human Pathology 45(2). p.382-393
Abstract
Acute tubular necrosis (ATN), elicited by ischemia and/or toxicity, is a potentially life-threatening condition. Histologically, ATN corresponds to necrosis and detachment of renal tubular epithelial cells. However, the tubules possess a considerable regenerative capacity and may be restored. We have previously identified a scattered population of progenitor-like cells within the proximal tubules, sharing marker expression with the parietal epithelial cells of Bowman's capsule as well as with renal tubules regenerating after ATN. In the present analysis, we use transmission electron microscopy, immunoelectron microscopy and immunofluorescence of human kidney cortex to further explore these cells. We demonstrate that the cells are smaller... (More)
Acute tubular necrosis (ATN), elicited by ischemia and/or toxicity, is a potentially life-threatening condition. Histologically, ATN corresponds to necrosis and detachment of renal tubular epithelial cells. However, the tubules possess a considerable regenerative capacity and may be restored. We have previously identified a scattered population of progenitor-like cells within the proximal tubules, sharing marker expression with the parietal epithelial cells of Bowman's capsule as well as with renal tubules regenerating after ATN. In the present analysis, we use transmission electron microscopy, immunoelectron microscopy and immunofluorescence of human kidney cortex to further explore these cells. We demonstrate that the cells are smaller and have drastically fewer mitochondria than the surrounding proximal tubule cells. They also display strong expression of several structural proteins such as vimentin, collagen-7A1 and the tight junction protein claudin-1. To functionally assess these cells, we also developed a novel human kidney explant model of ATN demonstrating that the cells are more resilient to injury than the surrounding proximal tubular cells. Taken together the results suggest a novel robust cell type with a contrasting biological role to that of the bulk of proximal tubular epithelium. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Human Pathology
volume
45
issue
2
pages
382 - 393
publisher
Elsevier
external identifiers
  • pmid:24439225
  • wos:000330551300024
  • scopus:84892760007
ISSN
1532-8392
DOI
10.1016/j.humpath.2013.10.003
language
English
LU publication?
yes
id
1a924845-5f32-43cf-8bdd-2eeae755a5b0 (old id 4291184)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24439225?dopt=Abstract
date added to LUP
2014-02-08 11:18:26
date last changed
2017-11-12 03:00:23
@article{1a924845-5f32-43cf-8bdd-2eeae755a5b0,
  abstract     = {Acute tubular necrosis (ATN), elicited by ischemia and/or toxicity, is a potentially life-threatening condition. Histologically, ATN corresponds to necrosis and detachment of renal tubular epithelial cells. However, the tubules possess a considerable regenerative capacity and may be restored. We have previously identified a scattered population of progenitor-like cells within the proximal tubules, sharing marker expression with the parietal epithelial cells of Bowman's capsule as well as with renal tubules regenerating after ATN. In the present analysis, we use transmission electron microscopy, immunoelectron microscopy and immunofluorescence of human kidney cortex to further explore these cells. We demonstrate that the cells are smaller and have drastically fewer mitochondria than the surrounding proximal tubule cells. They also display strong expression of several structural proteins such as vimentin, collagen-7A1 and the tight junction protein claudin-1. To functionally assess these cells, we also developed a novel human kidney explant model of ATN demonstrating that the cells are more resilient to injury than the surrounding proximal tubular cells. Taken together the results suggest a novel robust cell type with a contrasting biological role to that of the bulk of proximal tubular epithelium.},
  author       = {Hansson, Jennifer and Hultenby, Kjell and Cramnert, Catharina and Pontén, Fredrik and Jansson, Hannes and Lindgren, David and Axelson, Håkan and Johansson, Martin},
  issn         = {1532-8392},
  language     = {eng},
  number       = {2},
  pages        = {382--393},
  publisher    = {Elsevier},
  series       = {Human Pathology},
  title        = {Evidence for a morphologically distinct and functionally robust cell type in the proximal tubules of human kidney.},
  url          = {http://dx.doi.org/10.1016/j.humpath.2013.10.003},
  volume       = {45},
  year         = {2014},
}