Advanced

Prevention of Adhesions by PL/PG after Adhesiolysis

Åkerberg, Daniel LU ; Bauden, Monika LU ; Isaksson, Karolin LU ; Andersson, Roland LU and Tingstedt, Bobby LU (2012) In Journal of Tissue Science & Engineering 3(4).
Abstract
Background: Previous studies of differently charged polypeptides, Poly-L-lysine (PL) and Poly-L-Glutamate (PG) have shown promising results, reducing postoperative adhesions. This study aimed to investigate the possible anti adhesion effect of those combined polypeptides, after adhesiolys. The concentration of tPA, PAI-1 and active TGFb1 in biopsies from adhesions, unharmed peritoneum before and after adhesiolysis, was also investigated.

Materials and methods: A total of 24 male rats were divided in three groups A (N=8), B (N=8) and C (N=8). All rats underwent primary adhesion creating surgery at day 0, and adhesiolysis at day 7. Adhesions were evaluated at day 7 and 14, where group B received PL/PG after surgery at day 0 and... (More)
Background: Previous studies of differently charged polypeptides, Poly-L-lysine (PL) and Poly-L-Glutamate (PG) have shown promising results, reducing postoperative adhesions. This study aimed to investigate the possible anti adhesion effect of those combined polypeptides, after adhesiolys. The concentration of tPA, PAI-1 and active TGFb1 in biopsies from adhesions, unharmed peritoneum before and after adhesiolysis, was also investigated.

Materials and methods: A total of 24 male rats were divided in three groups A (N=8), B (N=8) and C (N=8). All rats underwent primary adhesion creating surgery at day 0, and adhesiolysis at day 7. Adhesions were evaluated at day 7 and 14, where group B received PL/PG after surgery at day 0 and after adhesiolysis at day 7, and group C received PL/PG after adhesiolysis at day 7. Tissue plasminogen activator (tPA), Plasminogen activator inhibitor 1(PAI-1) and active transforming growth factor beta 1(TGF-β1) were collected from biopsies of adhesions and normal peritoneum at day 0, 7 and 14.

Results: Significant reduction of adhesions p<0.05 was seen in group B at day 7 after primary surgery, and at day 14 after adhesiolysis. Significantly p<0.05 reduction of adhesions was seen at day 14 after adhesiolysis in group C. Some variations were seen in tPA, PAI-1 and active TGFb1.

Conclusions: PL/PG may be used to prevent adhesion formation after adhesiolysis. The process of fibrinolysis and fibrosis was not affected, after PL/PG prophylaxis and adhesiolysis. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to specialist publication or newspaper
publication status
published
subject
keywords
Abdominal adhesions, Prevention, Polypeptides, Adhesiolysis
categories
Popular Science
in
Journal of Tissue Science & Engineering
volume
3
issue
4
ISSN
2157-7552
DOI
10.4172/2157-7552.1000121
language
English
LU publication?
yes
id
ba539d12-87f6-4dad-ade3-e90362b579b9 (old id 4333697)
date added to LUP
2016-01-28 13:22:13
date last changed
2016-04-16 06:20:24
@misc{ba539d12-87f6-4dad-ade3-e90362b579b9,
  abstract     = {Background: Previous studies of differently charged polypeptides, Poly-L-lysine (PL) and Poly-L-Glutamate (PG) have shown promising results, reducing postoperative adhesions. This study aimed to investigate the possible anti adhesion effect of those combined polypeptides, after adhesiolys. The concentration of tPA, PAI-1 and active TGFb1 in biopsies from adhesions, unharmed peritoneum before and after adhesiolysis, was also investigated.<br/><br>
Materials and methods: A total of 24 male rats were divided in three groups A (N=8), B (N=8) and C (N=8). All rats underwent primary adhesion creating surgery at day 0, and adhesiolysis at day 7. Adhesions were evaluated at day 7 and 14, where group B received PL/PG after surgery at day 0 and after adhesiolysis at day 7, and group C received PL/PG after adhesiolysis at day 7. Tissue plasminogen activator (tPA), Plasminogen activator inhibitor 1(PAI-1) and active transforming growth factor beta 1(TGF-β1) were collected from biopsies of adhesions and normal peritoneum at day 0, 7 and 14.<br/><br>
Results: Significant reduction of adhesions p&lt;0.05 was seen in group B at day 7 after primary surgery, and at day 14 after adhesiolysis. Significantly p&lt;0.05 reduction of adhesions was seen at day 14 after adhesiolysis in group C. Some variations were seen in tPA, PAI-1 and active TGFb1.<br/><br>
Conclusions: PL/PG may be used to prevent adhesion formation after adhesiolysis. The process of fibrinolysis and fibrosis was not affected, after PL/PG prophylaxis and adhesiolysis.},
  author       = {Åkerberg, Daniel and Bauden, Monika and Isaksson, Karolin and Andersson, Roland and Tingstedt, Bobby},
  issn         = {2157-7552},
  keyword      = {Abdominal adhesions,Prevention,Polypeptides,Adhesiolysis},
  language     = {eng},
  number       = {4},
  series       = {Journal of Tissue Science & Engineering},
  title        = {Prevention of Adhesions by PL/PG after Adhesiolysis},
  url          = {http://dx.doi.org/10.4172/2157-7552.1000121},
  volume       = {3},
  year         = {2012},
}