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The influence on abdominal adhesions and inflammation in rabbits after exposure to differently charged polypeptides

Åkerberg, Daniel LU ; Grunditz, Carl; Bauden, Monika LU ; Isaksson, Karolin LU ; Andersson, Roland LU and Tingstedt, Bobby LU (2012) In Journal of Biomedical Science and Engineering 5. p.432-438
Abstract
Background: Abdominal adhesions develop on da- maged peritoneal surfaces and constitute a significant health related problem. Previous animal studies have shown promising anti-adhesive effects when adminis- tering the polycation α-poly-L-lysine (αPL) and the polyanion poly-L-glutamate (PG) together. The ob- jective of the study was to examine the effect of these differently charged polypeptides when administered by spraying and to evaluate any possible effect on fibrinolysis, fibrosis and inflammation. Methods: Rab- bits were treated with PLPG after cecal abrasive surgery and analysis from peritoneal biopsies of ac- tive tPa/PAI-1 complex and from peritoneal fluid of IL-6 and active TGFb1 at day 0, 1, 4 and 10 were measured after surgery.... (More)
Background: Abdominal adhesions develop on da- maged peritoneal surfaces and constitute a significant health related problem. Previous animal studies have shown promising anti-adhesive effects when adminis- tering the polycation α-poly-L-lysine (αPL) and the polyanion poly-L-glutamate (PG) together. The ob- jective of the study was to examine the effect of these differently charged polypeptides when administered by spraying and to evaluate any possible effect on fibrinolysis, fibrosis and inflammation. Methods: Rab- bits were treated with PLPG after cecal abrasive surgery and analysis from peritoneal biopsies of ac- tive tPa/PAI-1 complex and from peritoneal fluid of IL-6 and active TGFb1 at day 0, 1, 4 and 10 were measured after surgery. Histological specimens were analyzed on day 10 regarding inflammation and fib- rosis. Peritoneal adhesions were evaluated by adhe- sion score. All values were compared to the control group (NaCl). Results: PLPG-treated rabbits had a significant diminished adhesion score on day 10 as compared to the control group (p < 0.005). Signifi- cantly reduced collagen depositions on the perito- neum were seen in the PLPG group when evaluating the histological specimens (p < 0.05). No significant differences between the experimental and control groups were seen in peritoneal fluid when analyzing for active protein levels. Conclusion: This is the first study to investigate the effect on key parameters in adhesion formation as well as the preventive effect of the PLPG complex on abdominal adhesions in rabbits and also the first study where administration by spraying the polypeptides was used. PLPG was non- toxic in this setting and without significant differences in adhesion formation parameters and a significant reduction in adhesions was observed. This was veri- fied both macroscopically and histologically. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to specialist publication or newspaper
publication status
published
subject
keywords
Polycations, Abdominal Adhesions, Coagulation, Fibrosis, Inflammation
categories
Popular Science
in
Journal of Biomedical Science and Engineering
volume
5
pages
432 - 438
publisher
Scientific Research
ISSN
1937-6871
DOI
10.4236/jbise.2012.58055
language
English
LU publication?
yes
id
32c33c6c-5749-463c-8c93-0df3f04f63aa (old id 4333700)
date added to LUP
2016-01-28 12:27:29
date last changed
2016-06-09 11:11:37
@misc{32c33c6c-5749-463c-8c93-0df3f04f63aa,
  abstract     = {Background: Abdominal adhesions develop on da- maged peritoneal surfaces and constitute a significant health related problem. Previous animal studies have shown promising anti-adhesive effects when adminis- tering the polycation α-poly-L-lysine (αPL) and the polyanion poly-L-glutamate (PG) together. The ob- jective of the study was to examine the effect of these differently charged polypeptides when administered by spraying and to evaluate any possible effect on fibrinolysis, fibrosis and inflammation. Methods: Rab- bits were treated with PLPG after cecal abrasive surgery and analysis from peritoneal biopsies of ac- tive tPa/PAI-1 complex and from peritoneal fluid of IL-6 and active TGFb1 at day 0, 1, 4 and 10 were measured after surgery. Histological specimens were analyzed on day 10 regarding inflammation and fib- rosis. Peritoneal adhesions were evaluated by adhe- sion score. All values were compared to the control group (NaCl). Results: PLPG-treated rabbits had a significant diminished adhesion score on day 10 as compared to the control group (p &lt; 0.005). Signifi- cantly reduced collagen depositions on the perito- neum were seen in the PLPG group when evaluating the histological specimens (p &lt; 0.05). No significant differences between the experimental and control groups were seen in peritoneal fluid when analyzing for active protein levels. Conclusion: This is the first study to investigate the effect on key parameters in adhesion formation as well as the preventive effect of the PLPG complex on abdominal adhesions in rabbits and also the first study where administration by spraying the polypeptides was used. PLPG was non- toxic in this setting and without significant differences in adhesion formation parameters and a significant reduction in adhesions was observed. This was veri- fied both macroscopically and histologically.},
  author       = {Åkerberg, Daniel and Grunditz, Carl and Bauden, Monika and Isaksson, Karolin and Andersson, Roland and Tingstedt, Bobby},
  issn         = {1937-6871},
  keyword      = {Polycations,Abdominal Adhesions,Coagulation,Fibrosis,Inflammation},
  language     = {eng},
  pages        = {432--438},
  publisher    = {Scientific Research},
  series       = {Journal of Biomedical Science and Engineering},
  title        = {The influence on abdominal adhesions and inflammation in rabbits after exposure to differently charged polypeptides},
  url          = {http://dx.doi.org/10.4236/jbise.2012.58055},
  volume       = {5},
  year         = {2012},
}