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Extraction of water-soluble xylan from wheat bran and utilization of enzymatically produced xylooligosaccharides by Lactobacillus, Bifidobacterium and Weissella spp.

Immerzeel, Peter LU ; Falck, Peter LU ; Galbe, Mats LU ; Adlercreutz, Patrick LU ; Nordberg Karlsson, Eva LU and Stålbrand, Henrik LU (2014) In LWT- Food Science and Technology 56(2). p.321-327
Abstract
Xylan was extracted from wheat bran after heat pretreatment in water using either an autoclave or a microwave oven. Xylooligosaccharides (XOS) were produced from the xylan using the thermostable xylanase RmXyn10A and the potential prebiotic properties of XOS were studied in vitro with different human gut bacteria: Lactobacillus brevis (DSMZ 1269), Bifidobacterium adolescentis (ATCC 15703) and two strains of recently isolated lactic acid bacteria from the species pair Weissella cibaria/confusa. The highest yield of (arabino)xylan with the heat pretreatment was obtained at 185 degrees C for 10 min. Higher temperature led to fewer arabinose substitutions present on the backbone which in turn resulted in a slightly more efficient enzymatic... (More)
Xylan was extracted from wheat bran after heat pretreatment in water using either an autoclave or a microwave oven. Xylooligosaccharides (XOS) were produced from the xylan using the thermostable xylanase RmXyn10A and the potential prebiotic properties of XOS were studied in vitro with different human gut bacteria: Lactobacillus brevis (DSMZ 1269), Bifidobacterium adolescentis (ATCC 15703) and two strains of recently isolated lactic acid bacteria from the species pair Weissella cibaria/confusa. The highest yield of (arabino)xylan with the heat pretreatment was obtained at 185 degrees C for 10 min. Higher temperature led to fewer arabinose substitutions present on the backbone which in turn resulted in a slightly more efficient enzymatic hydrolysis by RmXyn10A. Using the produced XOS hydrolysate as carbon source, xylobiose uptake was confirmed for all bacterial species studied while xylotriose uptake could be confirmed for B. adolescentis and the Weissella strains. The negative control strain Escherichia coli (BL 21) did not use XOS as a carbon source. L brevis, B. adolescentis and the Weissella spp. all showed growth on XOS, verified by increases in cell density, lactic acid and acetic acid production after 48 h incubation. Corresponding increases were not found using the non-hydrolysed xylan as carbon source. (C) 2013 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Wheat bran, Xylan, Xylanase, Lactic acid bacteria, Weissella
in
LWT- Food Science and Technology
volume
56
issue
2
pages
321 - 327
publisher
Elsevier
external identifiers
  • wos:000331026100016
  • scopus:84892489487
ISSN
0023-6438
DOI
10.1016/j.lwt.2013.12.013
language
English
LU publication?
yes
id
0ad14a28-3476-45fd-8356-f3c570da40b2 (old id 4367245)
date added to LUP
2014-04-16 13:38:26
date last changed
2017-11-12 03:36:40
@article{0ad14a28-3476-45fd-8356-f3c570da40b2,
  abstract     = {Xylan was extracted from wheat bran after heat pretreatment in water using either an autoclave or a microwave oven. Xylooligosaccharides (XOS) were produced from the xylan using the thermostable xylanase RmXyn10A and the potential prebiotic properties of XOS were studied in vitro with different human gut bacteria: Lactobacillus brevis (DSMZ 1269), Bifidobacterium adolescentis (ATCC 15703) and two strains of recently isolated lactic acid bacteria from the species pair Weissella cibaria/confusa. The highest yield of (arabino)xylan with the heat pretreatment was obtained at 185 degrees C for 10 min. Higher temperature led to fewer arabinose substitutions present on the backbone which in turn resulted in a slightly more efficient enzymatic hydrolysis by RmXyn10A. Using the produced XOS hydrolysate as carbon source, xylobiose uptake was confirmed for all bacterial species studied while xylotriose uptake could be confirmed for B. adolescentis and the Weissella strains. The negative control strain Escherichia coli (BL 21) did not use XOS as a carbon source. L brevis, B. adolescentis and the Weissella spp. all showed growth on XOS, verified by increases in cell density, lactic acid and acetic acid production after 48 h incubation. Corresponding increases were not found using the non-hydrolysed xylan as carbon source. (C) 2013 Elsevier Ltd. All rights reserved.},
  author       = {Immerzeel, Peter and Falck, Peter and Galbe, Mats and Adlercreutz, Patrick and Nordberg Karlsson, Eva and Stålbrand, Henrik},
  issn         = {0023-6438},
  keyword      = {Wheat bran,Xylan,Xylanase,Lactic acid bacteria,Weissella},
  language     = {eng},
  number       = {2},
  pages        = {321--327},
  publisher    = {Elsevier},
  series       = {LWT- Food Science and Technology},
  title        = {Extraction of water-soluble xylan from wheat bran and utilization of enzymatically produced xylooligosaccharides by Lactobacillus, Bifidobacterium and Weissella spp.},
  url          = {http://dx.doi.org/10.1016/j.lwt.2013.12.013},
  volume       = {56},
  year         = {2014},
}