Extraction of water-soluble xylan from wheat bran and utilization of enzymatically produced xylooligosaccharides by Lactobacillus, Bifidobacterium and Weissella spp.
Immerzeel, Peter LU ; Falck, Peter LU ; Galbe, Mats LU ; Adlercreutz, Patrick LU
; Nordberg Karlsson, Eva
LU
and Stålbrand, Henrik
LU
(2014)
In LWT - Food Science and Technology
56(2).
p.321-327
- Abstract
- Xylan was extracted from wheat bran after heat pretreatment in water using either an autoclave or a microwave oven. Xylooligosaccharides (XOS) were produced from the xylan using the thermostable xylanase RmXyn10A and the potential prebiotic properties of XOS were studied in vitro with different human gut bacteria: Lactobacillus brevis (DSMZ 1269), Bifidobacterium adolescentis (ATCC 15703) and two strains of recently isolated lactic acid bacteria from the species pair Weissella cibaria/confusa. The highest yield of (arabino)xylan with the heat pretreatment was obtained at 185 degrees C for 10 min. Higher temperature led to fewer arabinose substitutions present on the backbone which in turn resulted in a slightly more efficient enzymatic... (More)
- Xylan was extracted from wheat bran after heat pretreatment in water using either an autoclave or a microwave oven. Xylooligosaccharides (XOS) were produced from the xylan using the thermostable xylanase RmXyn10A and the potential prebiotic properties of XOS were studied in vitro with different human gut bacteria: Lactobacillus brevis (DSMZ 1269), Bifidobacterium adolescentis (ATCC 15703) and two strains of recently isolated lactic acid bacteria from the species pair Weissella cibaria/confusa. The highest yield of (arabino)xylan with the heat pretreatment was obtained at 185 degrees C for 10 min. Higher temperature led to fewer arabinose substitutions present on the backbone which in turn resulted in a slightly more efficient enzymatic hydrolysis by RmXyn10A. Using the produced XOS hydrolysate as carbon source, xylobiose uptake was confirmed for all bacterial species studied while xylotriose uptake could be confirmed for B. adolescentis and the Weissella strains. The negative control strain Escherichia coli (BL 21) did not use XOS as a carbon source. L brevis, B. adolescentis and the Weissella spp. all showed growth on XOS, verified by increases in cell density, lactic acid and acetic acid production after 48 h incubation. Corresponding increases were not found using the non-hydrolysed xylan as carbon source. (C) 2013 Elsevier Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4367245
- author
- Immerzeel, Peter
LU
; Falck, Peter
LU
; Galbe, Mats
LU
; Adlercreutz, Patrick
LU
; Nordberg Karlsson, Eva
LU
and Stålbrand, Henrik
LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Wheat bran, Xylan, Xylanase, Lactic acid bacteria, Weissella
- in
- LWT - Food Science and Technology
- volume
- 56
- issue
- 2
- pages
- 321 - 327
- publisher
- Academic Press
- external identifiers
-
- wos:000331026100016
- scopus:84892489487
- ISSN
- 0023-6438
- DOI
- 10.1016/j.lwt.2013.12.013
- project
- ANTIDIABETIC FOOD CENTRE
- language
- English
- LU publication?
- yes
- id
- 0ad14a28-3476-45fd-8356-f3c570da40b2 (old id 4367245)
- date added to LUP
- 2016-04-01 13:19:34
- date last changed
- 2025-01-30 11:11:18
@article{0ad14a28-3476-45fd-8356-f3c570da40b2,
abstract = {{Xylan was extracted from wheat bran after heat pretreatment in water using either an autoclave or a microwave oven. Xylooligosaccharides (XOS) were produced from the xylan using the thermostable xylanase RmXyn10A and the potential prebiotic properties of XOS were studied in vitro with different human gut bacteria: Lactobacillus brevis (DSMZ 1269), Bifidobacterium adolescentis (ATCC 15703) and two strains of recently isolated lactic acid bacteria from the species pair Weissella cibaria/confusa. The highest yield of (arabino)xylan with the heat pretreatment was obtained at 185 degrees C for 10 min. Higher temperature led to fewer arabinose substitutions present on the backbone which in turn resulted in a slightly more efficient enzymatic hydrolysis by RmXyn10A. Using the produced XOS hydrolysate as carbon source, xylobiose uptake was confirmed for all bacterial species studied while xylotriose uptake could be confirmed for B. adolescentis and the Weissella strains. The negative control strain Escherichia coli (BL 21) did not use XOS as a carbon source. L brevis, B. adolescentis and the Weissella spp. all showed growth on XOS, verified by increases in cell density, lactic acid and acetic acid production after 48 h incubation. Corresponding increases were not found using the non-hydrolysed xylan as carbon source. (C) 2013 Elsevier Ltd. All rights reserved.}},
author = {{Immerzeel, Peter and Falck, Peter and Galbe, Mats and Adlercreutz, Patrick and Nordberg Karlsson, Eva and Stålbrand, Henrik}},
issn = {{0023-6438}},
keywords = {{Wheat bran; Xylan; Xylanase; Lactic acid bacteria; Weissella}},
language = {{eng}},
number = {{2}},
pages = {{321--327}},
publisher = {{Academic Press}},
series = {{LWT - Food Science and Technology}},
title = {{Extraction of water-soluble xylan from wheat bran and utilization of enzymatically produced xylooligosaccharides by Lactobacillus, Bifidobacterium and Weissella spp.}},
url = {{http://dx.doi.org/10.1016/j.lwt.2013.12.013}},
doi = {{10.1016/j.lwt.2013.12.013}},
volume = {{56}},
year = {{2014}},
}