Novel cardiovascular biomarkers associated with peripheral arterial disease in men screened for abdominal aortic aneurysm
(2022) In Vasa - European Journal of Vascular Medicine 51(3). p.167-173- Abstract
Background: Peripheral arterial disease (PAD) is a common atherosclerotic disease with severity ranging from asymptomatic to chronic limb threatening ischemia. The aim of the present cross-sectional study was to identify novel biomarkers associated with PAD. Patients and methods: Levels of 91 cardiovascular specific proteins in plasma samples were measured by the Proseek Multiplex CVD III96x96 panel from a cohort consisting of 267 65-year-old men recruited from a screening program for abdominal aortic aneurysm (AAA) Levels of protein biomarkers were compared in men with and without PAD (defined as an ankle brachial index of <0.9) and their diagnostic potential was calculated by receiver-operating characteristic analysis. Results:... (More)
Background: Peripheral arterial disease (PAD) is a common atherosclerotic disease with severity ranging from asymptomatic to chronic limb threatening ischemia. The aim of the present cross-sectional study was to identify novel biomarkers associated with PAD. Patients and methods: Levels of 91 cardiovascular specific proteins in plasma samples were measured by the Proseek Multiplex CVD III96x96 panel from a cohort consisting of 267 65-year-old men recruited from a screening program for abdominal aortic aneurysm (AAA) Levels of protein biomarkers were compared in men with and without PAD (defined as an ankle brachial index of <0.9) and their diagnostic potential was calculated by receiver-operating characteristic analysis. Results: The prevalence of PAD was 14.2% (38/267). After adjustment for multiple comparisons, levels of the following 11 biomarkers remained significantly higher (p<0.0001) in patients with PAD: secretoglobin family 3A member 2, osteoprotegerin, urokinase-type plasminogen activator surface receptor, serum macrophage chemokine ligand 16, matrix metalloproteinase 9, p-selectin, growth differentiation factor 15, elafin, cystatin B, trefoil factor 3, and fatty acid-binding protein 4. Multivariable logistic regression analysis (adjusted for smoking, use of antihypertensive and lipid-lowering medication, and metformin) showed that 11 biomarkers were significantly associated with higher risk of PAD with odds ratios ranging from 1.6 to 2.4. Area under curve calculated by receiver operating characteristic curve analysis (diagnostic value) for each protein biomarker ranged from 0.63 to 0.74. Conclusions: We have identified multiple proteins with a potential to be diagnostic biomarkers for PAD, and further research is warranted to clarify their potential predictive and prognostic value.
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- author
- Dakhel, Ardwan LU ; Memon, Ashfaque A LU ; Zarrouk, Moncef LU ; Ågren-Witteschus, Sophia LU ; Sundquist, Jan LU ; Sundquist, Kristina LU and Gottsäter, Anders LU
- organization
- publishing date
- 2022-04-07
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Biomarkers, peripheral arterial disease, abdominal aortic aneurysm
- in
- Vasa - European Journal of Vascular Medicine
- volume
- 51
- issue
- 3
- pages
- 167 - 173
- publisher
- Verlag Hans Huber
- external identifiers
-
- pmid:35387491
- scopus:85128766104
- ISSN
- 0301-1526
- DOI
- 10.1024/0301-1526/a000999
- language
- English
- LU publication?
- yes
- id
- 436f4b00-599e-4f14-b2e2-016ae2a3c5f1
- date added to LUP
- 2022-04-14 20:09:12
- date last changed
- 2024-09-20 02:15:55
@article{436f4b00-599e-4f14-b2e2-016ae2a3c5f1, abstract = {{<p> Background: Peripheral arterial disease (PAD) is a common atherosclerotic disease with severity ranging from asymptomatic to chronic limb threatening ischemia. The aim of the present cross-sectional study was to identify novel biomarkers associated with PAD. Patients and methods: Levels of 91 cardiovascular specific proteins in plasma samples were measured by the Proseek Multiplex CVD III96x96 panel from a cohort consisting of 267 65-year-old men recruited from a screening program for abdominal aortic aneurysm (AAA) Levels of protein biomarkers were compared in men with and without PAD (defined as an ankle brachial index of <0.9) and their diagnostic potential was calculated by receiver-operating characteristic analysis. Results: The prevalence of PAD was 14.2% (38/267). After adjustment for multiple comparisons, levels of the following 11 biomarkers remained significantly higher (p<0.0001) in patients with PAD: secretoglobin family 3A member 2, osteoprotegerin, urokinase-type plasminogen activator surface receptor, serum macrophage chemokine ligand 16, matrix metalloproteinase 9, p-selectin, growth differentiation factor 15, elafin, cystatin B, trefoil factor 3, and fatty acid-binding protein 4. Multivariable logistic regression analysis (adjusted for smoking, use of antihypertensive and lipid-lowering medication, and metformin) showed that 11 biomarkers were significantly associated with higher risk of PAD with odds ratios ranging from 1.6 to 2.4. Area under curve calculated by receiver operating characteristic curve analysis (diagnostic value) for each protein biomarker ranged from 0.63 to 0.74. Conclusions: We have identified multiple proteins with a potential to be diagnostic biomarkers for PAD, and further research is warranted to clarify their potential predictive and prognostic value.</p>}}, author = {{Dakhel, Ardwan and Memon, Ashfaque A and Zarrouk, Moncef and Ågren-Witteschus, Sophia and Sundquist, Jan and Sundquist, Kristina and Gottsäter, Anders}}, issn = {{0301-1526}}, keywords = {{Biomarkers; peripheral arterial disease; abdominal aortic aneurysm}}, language = {{eng}}, month = {{04}}, number = {{3}}, pages = {{167--173}}, publisher = {{Verlag Hans Huber}}, series = {{Vasa - European Journal of Vascular Medicine}}, title = {{Novel cardiovascular biomarkers associated with peripheral arterial disease in men screened for abdominal aortic aneurysm}}, url = {{http://dx.doi.org/10.1024/0301-1526/a000999}}, doi = {{10.1024/0301-1526/a000999}}, volume = {{51}}, year = {{2022}}, }