Advanced

Supported liquid membrane as a novel tool for driving the equilibrium of ω-transaminase catalyzed asymmetric synthesis.

Rehn, Gustav LU ; Adlercreutz, Patrick LU and Grey, Carl LU (2014) In Journal of Biotechnology 179. p.50-55
Abstract
An attractive option to produce chiral amines of industrial importance is through asymmetric synthesis using ω-transaminase. However, reaching high yields often requires a strategy for shifting the equilibrium position. This paper describes a novel strategy for handling this problem. It involves the use of a supported liquid membrane (SLM) together with a packed bed reactor. The reactor contains Escherichia coli cells with ω-transaminase from Arthrobacter citreus, immobilized by flocculation with chitosan. The SLM consists of a hollow fibre membrane contactor in which the pores contain undecane. The system enables continuous extraction of the amine product and was used to successfully shift the equilibrium in asymmetric synthesis of... (More)
An attractive option to produce chiral amines of industrial importance is through asymmetric synthesis using ω-transaminase. However, reaching high yields often requires a strategy for shifting the equilibrium position. This paper describes a novel strategy for handling this problem. It involves the use of a supported liquid membrane (SLM) together with a packed bed reactor. The reactor contains Escherichia coli cells with ω-transaminase from Arthrobacter citreus, immobilized by flocculation with chitosan. The SLM consists of a hollow fibre membrane contactor in which the pores contain undecane. The system enables continuous extraction of the amine product and was used to successfully shift the equilibrium in asymmetric synthesis of (S)-α-methylbenzylamine (MBA). A conversion of 98% was reached, compared to 50% without product extraction. Moreover, a selective extraction of the produced MBA was realized. A high product concentration of 55g/l was reached after 80h, and the system showed promising potential for continuous operation. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biotechnology
volume
179
pages
50 - 55
publisher
Elsevier
external identifiers
  • pmid:24675224
  • wos:000335557800008
  • scopus:84897408319
ISSN
1873-4863
DOI
10.1016/j.jbiotec.2014.03.022
language
English
LU publication?
yes
id
1f83d2ac-d90c-4c64-847b-aaf36794751d (old id 4379579)
date added to LUP
2014-04-28 11:26:31
date last changed
2017-10-29 03:12:41
@article{1f83d2ac-d90c-4c64-847b-aaf36794751d,
  abstract     = {An attractive option to produce chiral amines of industrial importance is through asymmetric synthesis using ω-transaminase. However, reaching high yields often requires a strategy for shifting the equilibrium position. This paper describes a novel strategy for handling this problem. It involves the use of a supported liquid membrane (SLM) together with a packed bed reactor. The reactor contains Escherichia coli cells with ω-transaminase from Arthrobacter citreus, immobilized by flocculation with chitosan. The SLM consists of a hollow fibre membrane contactor in which the pores contain undecane. The system enables continuous extraction of the amine product and was used to successfully shift the equilibrium in asymmetric synthesis of (S)-α-methylbenzylamine (MBA). A conversion of 98% was reached, compared to 50% without product extraction. Moreover, a selective extraction of the produced MBA was realized. A high product concentration of 55g/l was reached after 80h, and the system showed promising potential for continuous operation.},
  author       = {Rehn, Gustav and Adlercreutz, Patrick and Grey, Carl},
  issn         = {1873-4863},
  language     = {eng},
  pages        = {50--55},
  publisher    = {Elsevier},
  series       = {Journal of Biotechnology},
  title        = {Supported liquid membrane as a novel tool for driving the equilibrium of ω-transaminase catalyzed asymmetric synthesis.},
  url          = {http://dx.doi.org/10.1016/j.jbiotec.2014.03.022},
  volume       = {179},
  year         = {2014},
}