Neutrophils from vasculitis patients exhibit an increased propensity for activation by anti-neutrophil cytoplasmic antibodies.
(2014) In Clinical and Experimental Immunology 176(3). p.363-372- Abstract
- Anti-neutrophil cytoplasmic antibodies (ANCA) are thought to be pathogenic in ANCA associated vasculitis (AAV) by stimulating polymorphonuclear leukocytes (PMNs) to degranulate and produce reactive oxygen species (ROS). The aim of this study was to investigate if PMNs from AAV patients are more readily stimulated by ANCA compared with PMNs from healthy controls (HCs). Differences in ANCA characteristics that can account for different stimulation potential were also studied. PMNs from 5 AAV patients and 5 HCs were stimulated with 10 different IgGs, purified from PR3-ANCA positive patients, and ROS production, degranulation and neutrophil extracellular trap (NET) formation was measured. ANCA levels, affinity, and clinical data of the AAV... (More)
- Anti-neutrophil cytoplasmic antibodies (ANCA) are thought to be pathogenic in ANCA associated vasculitis (AAV) by stimulating polymorphonuclear leukocytes (PMNs) to degranulate and produce reactive oxygen species (ROS). The aim of this study was to investigate if PMNs from AAV patients are more readily stimulated by ANCA compared with PMNs from healthy controls (HCs). Differences in ANCA characteristics that can account for different stimulation potential were also studied. PMNs from 5 AAV patients and 5 HCs were stimulated with 10 different IgGs, purified from PR3-ANCA positive patients, and ROS production, degranulation and neutrophil extracellular trap (NET) formation was measured. ANCA levels, affinity, and clinical data of the AAV donors were recorded. The results show that PMNs from AAV patients produce more intracellular ROS (p=0.019), but degranulate to a similar extent as PMNs from HCs. ROS production correlated with NET formation. Factors that may influence the ability of ANCA to activate PMNs include affinity and specificity for N-terminal epitopes. In conclusion, our results indicate that PMNs from AAV patients in remission behave quite similar to HC PMNs, with the exception of a greater intracellular ROS production. This could contribute to more extensive NET formation and thus an increased exposure of the ANCA autoantigens to the immune system. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4379740
- author
- Ohlsson, Susanne
LU
; Ohlsson, Sophie
LU
; Söderberg, Daniel ; Gunnarsson, Lena LU ; Pettersson, Åsa LU ; Segelmark, Mårten LU
and Hellmark, Thomas LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Clinical and Experimental Immunology
- volume
- 176
- issue
- 3
- pages
- 363 - 372
- publisher
- British Society for Immunology
- external identifiers
-
- pmid:24666336
- wos:000337516700008
- scopus:84899092051
- ISSN
- 0009-9104
- DOI
- 10.1111/cei.12301
- language
- English
- LU publication?
- yes
- id
- 7ef541a7-79a1-4faf-802e-cb50b16a5f2f (old id 4379740)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24666336?dopt=Abstract
- date added to LUP
- 2016-04-01 11:08:54
- date last changed
- 2024-11-05 00:11:13
@article{7ef541a7-79a1-4faf-802e-cb50b16a5f2f, abstract = {{Anti-neutrophil cytoplasmic antibodies (ANCA) are thought to be pathogenic in ANCA associated vasculitis (AAV) by stimulating polymorphonuclear leukocytes (PMNs) to degranulate and produce reactive oxygen species (ROS). The aim of this study was to investigate if PMNs from AAV patients are more readily stimulated by ANCA compared with PMNs from healthy controls (HCs). Differences in ANCA characteristics that can account for different stimulation potential were also studied. PMNs from 5 AAV patients and 5 HCs were stimulated with 10 different IgGs, purified from PR3-ANCA positive patients, and ROS production, degranulation and neutrophil extracellular trap (NET) formation was measured. ANCA levels, affinity, and clinical data of the AAV donors were recorded. The results show that PMNs from AAV patients produce more intracellular ROS (p=0.019), but degranulate to a similar extent as PMNs from HCs. ROS production correlated with NET formation. Factors that may influence the ability of ANCA to activate PMNs include affinity and specificity for N-terminal epitopes. In conclusion, our results indicate that PMNs from AAV patients in remission behave quite similar to HC PMNs, with the exception of a greater intracellular ROS production. This could contribute to more extensive NET formation and thus an increased exposure of the ANCA autoantigens to the immune system.}}, author = {{Ohlsson, Susanne and Ohlsson, Sophie and Söderberg, Daniel and Gunnarsson, Lena and Pettersson, Åsa and Segelmark, Mårten and Hellmark, Thomas}}, issn = {{0009-9104}}, language = {{eng}}, number = {{3}}, pages = {{363--372}}, publisher = {{British Society for Immunology}}, series = {{Clinical and Experimental Immunology}}, title = {{Neutrophils from vasculitis patients exhibit an increased propensity for activation by anti-neutrophil cytoplasmic antibodies.}}, url = {{http://dx.doi.org/10.1111/cei.12301}}, doi = {{10.1111/cei.12301}}, volume = {{176}}, year = {{2014}}, }