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Serum from patients with systemic vasculitis induces alternatively activated macrophage M2c polarization.

Ohlsson, Susanne LU ; Linge, Petrus LU ; Gullstrand, Birgitta LU ; Lood, Christian LU ; Johansson, Åsa LU ; Ohlsson, Sophie LU ; Lundqvist, Andrea; Bengtsson, Anders LU ; Carlsson, Fredric and Hellmark, Thomas LU (2014) In Clinical Immunology 152(1-2). p.10-19
Abstract
Anti-neutrophil cytoplasmic antibody associated vasculitides (AAV) are conditions defined by an autoimmune small vessel inflammation. Dying neutrophils are found around the inflamed vessels and the balance between infiltrating neutrophils and macrophages is important to prevent autoimmunity. Here we investigate how sera from AAV patients may regulate macrophage polarization and function. Macrophages from healthy individuals were differentiated into M0, M1, M2a, M2b or M2c macrophages using a standardized protocol, and phenotyped according to their expression surface markers and cytokine production. These phenotypes were compared with those of macrophages stimulated with serum from AAV patients or healthy controls. While the healthy control... (More)
Anti-neutrophil cytoplasmic antibody associated vasculitides (AAV) are conditions defined by an autoimmune small vessel inflammation. Dying neutrophils are found around the inflamed vessels and the balance between infiltrating neutrophils and macrophages is important to prevent autoimmunity. Here we investigate how sera from AAV patients may regulate macrophage polarization and function. Macrophages from healthy individuals were differentiated into M0, M1, M2a, M2b or M2c macrophages using a standardized protocol, and phenotyped according to their expression surface markers and cytokine production. These phenotypes were compared with those of macrophages stimulated with serum from AAV patients or healthy controls. While the healthy control sera induced a M0 macrophage, AAV serum promoted polarization towards the M2c subtype. No sera induced M1, M2a or M2b macrophages. The M2c subtype showed increased phagocytosis capacity compared with the other subtypes. The M2c polarization found in AAV is consistent with previous reports of increased levels of M2c-associated cytokines. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Clinical Immunology
volume
152
issue
1-2
pages
10 - 19
publisher
Elsevier
external identifiers
  • pmid:24631966
  • wos:000335291400002
  • scopus:84897005856
ISSN
1521-6616
DOI
10.1016/j.clim.2014.02.016
language
English
LU publication?
yes
id
bd9638dd-e87a-45b8-bfba-248a5e1297c0 (old id 4383182)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24631966?dopt=Abstract
date added to LUP
2014-04-03 16:26:19
date last changed
2017-10-29 03:14:41
@article{bd9638dd-e87a-45b8-bfba-248a5e1297c0,
  abstract     = {Anti-neutrophil cytoplasmic antibody associated vasculitides (AAV) are conditions defined by an autoimmune small vessel inflammation. Dying neutrophils are found around the inflamed vessels and the balance between infiltrating neutrophils and macrophages is important to prevent autoimmunity. Here we investigate how sera from AAV patients may regulate macrophage polarization and function. Macrophages from healthy individuals were differentiated into M0, M1, M2a, M2b or M2c macrophages using a standardized protocol, and phenotyped according to their expression surface markers and cytokine production. These phenotypes were compared with those of macrophages stimulated with serum from AAV patients or healthy controls. While the healthy control sera induced a M0 macrophage, AAV serum promoted polarization towards the M2c subtype. No sera induced M1, M2a or M2b macrophages. The M2c subtype showed increased phagocytosis capacity compared with the other subtypes. The M2c polarization found in AAV is consistent with previous reports of increased levels of M2c-associated cytokines.},
  author       = {Ohlsson, Susanne and Linge, Petrus and Gullstrand, Birgitta and Lood, Christian and Johansson, Åsa and Ohlsson, Sophie and Lundqvist, Andrea and Bengtsson, Anders and Carlsson, Fredric and Hellmark, Thomas},
  issn         = {1521-6616},
  language     = {eng},
  number       = {1-2},
  pages        = {10--19},
  publisher    = {Elsevier},
  series       = {Clinical Immunology},
  title        = {Serum from patients with systemic vasculitis induces alternatively activated macrophage M2c polarization.},
  url          = {http://dx.doi.org/10.1016/j.clim.2014.02.016},
  volume       = {152},
  year         = {2014},
}