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Associations between serum phthalates and biomarkers of reproductive function in 589 adult men.

Specht, Ina Olmer; Toft, Gunnar; Hougaard, Karin S; Lindh, Christian LU ; Lenters, Virissa; Jönsson, Bo A LU ; Heederik, Dick; Giwercman, Aleksander LU and Bonde, Jens Peter E (2014) In Environment International 66(Feb 26). p.146-156
Abstract
Phthalates which are widely used, are ubiquitous in the environment and in some human tissues. It is generally accepted that phthalates exert their toxic action by inhibiting Leydig cell synthesis of testosterone, but in vitro studies have also shown anti-androgenic effects at the receptor level. Some cross-sectional studies have shown inverse associations between urinary levels of phthalates and reproductive hormones, but results are conflicting and the evidence base is limited. The aim of this study was to investigate if levels of di-2-ethylhexyl phthalate (DEHP) and diisononyl phthalate (DiNP) metabolites in serum are associated with serum concentrations of male reproductive hormones and semen quality. A secondary aim was to investigate... (More)
Phthalates which are widely used, are ubiquitous in the environment and in some human tissues. It is generally accepted that phthalates exert their toxic action by inhibiting Leydig cell synthesis of testosterone, but in vitro studies have also shown anti-androgenic effects at the receptor level. Some cross-sectional studies have shown inverse associations between urinary levels of phthalates and reproductive hormones, but results are conflicting and the evidence base is limited. The aim of this study was to investigate if levels of di-2-ethylhexyl phthalate (DEHP) and diisononyl phthalate (DiNP) metabolites in serum are associated with serum concentrations of male reproductive hormones and semen quality. A secondary aim was to investigate metabolic pathways of DEHP and DiNP on semen quality and reproductive hormones. A cross-sectional sample of 589 spouses of pregnant women from Greenland, Poland and Ukraine were enrolled between 2002 and 2004. The men gave semen and blood samples and were interviewed. Six phthalate metabolites of DEHP and DiNP were measured by liquid chromatography tandem mass spectrometry in serum. The metabolites were summed according to their molar weight. We observed significant inverse associations between serum levels of the metabolites, the proxies and serum testosterone. Negative associations were also discovered between some metabolites and sex hormone-binding globulin, semen volume and total sperm count. Findings are compatible with a weak anti-androgenic action of DEHP metabolites, but less so for DiNP metabolites. Metabolic pathways differed significantly between the three study sites, but without major effect on semen quality or reproductive hormones. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Environment International
volume
66
issue
Feb 26
pages
146 - 156
publisher
Elsevier
external identifiers
  • pmid:24583187
  • wos:000335108900018
  • scopus:84894547298
ISSN
1873-6750
DOI
10.1016/j.envint.2014.02.002
language
English
LU publication?
yes
id
f68c33b8-1338-4b81-bc5c-237d775f78e3 (old id 4384346)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24583187?dopt=Abstract
date added to LUP
2014-04-01 17:51:14
date last changed
2017-07-23 03:12:02
@article{f68c33b8-1338-4b81-bc5c-237d775f78e3,
  abstract     = {Phthalates which are widely used, are ubiquitous in the environment and in some human tissues. It is generally accepted that phthalates exert their toxic action by inhibiting Leydig cell synthesis of testosterone, but in vitro studies have also shown anti-androgenic effects at the receptor level. Some cross-sectional studies have shown inverse associations between urinary levels of phthalates and reproductive hormones, but results are conflicting and the evidence base is limited. The aim of this study was to investigate if levels of di-2-ethylhexyl phthalate (DEHP) and diisononyl phthalate (DiNP) metabolites in serum are associated with serum concentrations of male reproductive hormones and semen quality. A secondary aim was to investigate metabolic pathways of DEHP and DiNP on semen quality and reproductive hormones. A cross-sectional sample of 589 spouses of pregnant women from Greenland, Poland and Ukraine were enrolled between 2002 and 2004. The men gave semen and blood samples and were interviewed. Six phthalate metabolites of DEHP and DiNP were measured by liquid chromatography tandem mass spectrometry in serum. The metabolites were summed according to their molar weight. We observed significant inverse associations between serum levels of the metabolites, the proxies and serum testosterone. Negative associations were also discovered between some metabolites and sex hormone-binding globulin, semen volume and total sperm count. Findings are compatible with a weak anti-androgenic action of DEHP metabolites, but less so for DiNP metabolites. Metabolic pathways differed significantly between the three study sites, but without major effect on semen quality or reproductive hormones.},
  author       = {Specht, Ina Olmer and Toft, Gunnar and Hougaard, Karin S and Lindh, Christian and Lenters, Virissa and Jönsson, Bo A and Heederik, Dick and Giwercman, Aleksander and Bonde, Jens Peter E},
  issn         = {1873-6750},
  language     = {eng},
  number       = {Feb 26},
  pages        = {146--156},
  publisher    = {Elsevier},
  series       = {Environment International},
  title        = {Associations between serum phthalates and biomarkers of reproductive function in 589 adult men.},
  url          = {http://dx.doi.org/10.1016/j.envint.2014.02.002},
  volume       = {66},
  year         = {2014},
}