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The MAP kinase substrate MKS1 is a regulator of plant defence responces

Andreasson, Erik LU ; Jenkins, Thomas ; Brodersen, Peter ; Thorgrimsen, Stephan ; Petersen, Nikolaj HT ; Zhu, Shijiang ; Qiu, Jin-Long ; Qiu, Jin-Long ; Micheelsen, Pernille and Rocher, Anne , et al. (2005) In EMBO Journal 24(14). p.2579-2589
Abstract
Arabidopsis MAP kinase 4 (MPK4) functions as a regulator of pathogen defense responses, because it is required for both repression of salicylic acid (SA)-dependent resistance and for activation of jasmonate (JA)-dependent defense gene expression. To understand MPK4 signaling mechanisms, we used yeast two-hybrid screening to identify the MPK4 substrate MKS1. Analyses of transgenic plants and genome-wide transcript profiling indicated that MKS1 is required for full SA-dependent resistance in mpk4 mutants, and that overexpression of MKS1 in wild-type plants is sufficient to activate SA-dependent resistance, but does not interfere with induction of a defense gene by JA. Further yeast two-hybrid screening revealed that MKS1 interacts with the... (More)
Arabidopsis MAP kinase 4 (MPK4) functions as a regulator of pathogen defense responses, because it is required for both repression of salicylic acid (SA)-dependent resistance and for activation of jasmonate (JA)-dependent defense gene expression. To understand MPK4 signaling mechanisms, we used yeast two-hybrid screening to identify the MPK4 substrate MKS1. Analyses of transgenic plants and genome-wide transcript profiling indicated that MKS1 is required for full SA-dependent resistance in mpk4 mutants, and that overexpression of MKS1 in wild-type plants is sufficient to activate SA-dependent resistance, but does not interfere with induction of a defense gene by JA. Further yeast two-hybrid screening revealed that MKS1 interacts with the WRKY transcription factors WRKY25 and WRKY33. WRKY25 and WRKY33 were shown to be in vitro substrates of MPK4, and a wrky33 knockout mutant was found to exhibit increased expression of the SA-related defense gene PR1. MKS1 may therefore contribute to MPK4-regulated defense activation by coupling the kinase to specific WRKY transcription factors. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
protein kinase, phosphoserine, disease resistance
in
EMBO Journal
volume
24
issue
14
pages
2579 - 2589
publisher
Oxford University Press
external identifiers
  • scopus:23044508877
  • pmid:15990873
ISSN
1460-2075
DOI
10.1038/sj.emboj.7600737
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biology building (Closed 2011) (011008000)
id
43fb4b47-b4dd-41a4-9ff6-f3e6e918031e (old id 639690)
date added to LUP
2016-04-01 11:56:35
date last changed
2022-04-28 22:12:13
@article{43fb4b47-b4dd-41a4-9ff6-f3e6e918031e,
  abstract     = {{Arabidopsis MAP kinase 4 (MPK4) functions as a regulator of pathogen defense responses, because it is required for both repression of salicylic acid (SA)-dependent resistance and for activation of jasmonate (JA)-dependent defense gene expression. To understand MPK4 signaling mechanisms, we used yeast two-hybrid screening to identify the MPK4 substrate MKS1. Analyses of transgenic plants and genome-wide transcript profiling indicated that MKS1 is required for full SA-dependent resistance in mpk4 mutants, and that overexpression of MKS1 in wild-type plants is sufficient to activate SA-dependent resistance, but does not interfere with induction of a defense gene by JA. Further yeast two-hybrid screening revealed that MKS1 interacts with the WRKY transcription factors WRKY25 and WRKY33. WRKY25 and WRKY33 were shown to be in vitro substrates of MPK4, and a wrky33 knockout mutant was found to exhibit increased expression of the SA-related defense gene PR1. MKS1 may therefore contribute to MPK4-regulated defense activation by coupling the kinase to specific WRKY transcription factors.}},
  author       = {{Andreasson, Erik and Jenkins, Thomas and Brodersen, Peter and Thorgrimsen, Stephan and Petersen, Nikolaj HT and Zhu, Shijiang and Qiu, Jin-Long and Qiu, Jin-Long and Micheelsen, Pernille and Rocher, Anne and Petersen, Morten and Newman, Mari-Anne and Nielsen, Henrik Bjørn and Hirt, Heribert and Somssich, Imre and Mattsson, Ole and Mundy, John}},
  issn         = {{1460-2075}},
  keywords     = {{protein kinase; phosphoserine; disease resistance}},
  language     = {{eng}},
  number       = {{14}},
  pages        = {{2579--2589}},
  publisher    = {{Oxford University Press}},
  series       = {{EMBO Journal}},
  title        = {{The MAP kinase substrate MKS1 is a regulator of plant defence responces}},
  url          = {{http://dx.doi.org/10.1038/sj.emboj.7600737}},
  doi          = {{10.1038/sj.emboj.7600737}},
  volume       = {{24}},
  year         = {{2005}},
}