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A perspective on the characterization of colloids and macromolecules using asymmetrical flow field-flow fractionation

Runyon, Ray LU ; Ulmius, Matilda and Nilsson, Lars LU (2014) In Colloids and Surfaces A: Physicochemical and Engineering Aspects 442. p.25-33
Abstract
Asymmetrical flow field-flow fractionation (AF4) is rapidly becoming a technique of choice for the separation and characterization of complex materials. It is capable of fractionating samples over a wide size (similar to 2 nm to 50 mu m in diameter) and molecular weight range (10(3)-10(10) g/mol). It offers gentle, low shear, low pressure separation conditions which are essential to preserve the structure and aggregation of fragile species. In this paper we illustrate a number of examples where AF4 plays central role in providing detailed and accurate characterization of polydisperse and complex colloidal and macromolecular materials such as proteins, polysaccharides, nanoparticles, and emulsion droplets. (C) 2013 Elsevier B.V. All rights... (More)
Asymmetrical flow field-flow fractionation (AF4) is rapidly becoming a technique of choice for the separation and characterization of complex materials. It is capable of fractionating samples over a wide size (similar to 2 nm to 50 mu m in diameter) and molecular weight range (10(3)-10(10) g/mol). It offers gentle, low shear, low pressure separation conditions which are essential to preserve the structure and aggregation of fragile species. In this paper we illustrate a number of examples where AF4 plays central role in providing detailed and accurate characterization of polydisperse and complex colloidal and macromolecular materials such as proteins, polysaccharides, nanoparticles, and emulsion droplets. (C) 2013 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Field-flow fractionation, Colloids, Proteins, Beta-glucans, Aggregates, Emulsions
in
Colloids and Surfaces A: Physicochemical and Engineering Aspects
volume
442
pages
25 - 33
publisher
Elsevier
external identifiers
  • wos:000331687000005
  • scopus:84895065481
ISSN
0927-7757
DOI
10.1016/j.colsurfa.2013.04.010
language
English
LU publication?
yes
id
62f36900-e4d7-442e-9ce4-2831eaccb176 (old id 4418903)
date added to LUP
2014-05-05 10:55:24
date last changed
2017-11-05 04:11:40
@article{62f36900-e4d7-442e-9ce4-2831eaccb176,
  abstract     = {Asymmetrical flow field-flow fractionation (AF4) is rapidly becoming a technique of choice for the separation and characterization of complex materials. It is capable of fractionating samples over a wide size (similar to 2 nm to 50 mu m in diameter) and molecular weight range (10(3)-10(10) g/mol). It offers gentle, low shear, low pressure separation conditions which are essential to preserve the structure and aggregation of fragile species. In this paper we illustrate a number of examples where AF4 plays central role in providing detailed and accurate characterization of polydisperse and complex colloidal and macromolecular materials such as proteins, polysaccharides, nanoparticles, and emulsion droplets. (C) 2013 Elsevier B.V. All rights reserved.},
  author       = {Runyon, Ray and Ulmius, Matilda and Nilsson, Lars},
  issn         = {0927-7757},
  keyword      = {Field-flow fractionation,Colloids,Proteins,Beta-glucans,Aggregates,Emulsions},
  language     = {eng},
  pages        = {25--33},
  publisher    = {Elsevier},
  series       = {Colloids and Surfaces A: Physicochemical and Engineering Aspects},
  title        = {A perspective on the characterization of colloids and macromolecules using asymmetrical flow field-flow fractionation},
  url          = {http://dx.doi.org/10.1016/j.colsurfa.2013.04.010},
  volume       = {442},
  year         = {2014},
}