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Quantifying the release of lactose from polymer matrix tablets with an amperometric biosensor utilizing cellobiose dehydrogenase.

Knöös, Patrik LU ; Schulz, Christopher LU ; Piculell, Lennart LU ; Ludwig, Roland; Gorton, Lo LU and Wahlgren, Marie LU (2014) In International Journal of Pharmaceutics 468(1-2). p.121-132
Abstract
The release of lactose (hydrophilic) from polymer tablets made with hydrophobically modified poly(acrylic acid) (HMPAA) have been studied and compared it to the release of ibuprofen, a hydrophobic active substance. Lactose is one of the most used excipients for tablets, but lactose release has not been widely studied. One reason could be a lack of good analytical tools. A novel biosensor with cellobiose dehydrogenase (CDH) was used to detect the lactose release, which has a polydiallyldimethylammonium chloride (PDADMAC) layer that increases the response. A sample treatment using polyethylenimine (PEI) was developed to eliminate possible denaturants. The developed methodology provided a good approach to detect and quantify the released... (More)
The release of lactose (hydrophilic) from polymer tablets made with hydrophobically modified poly(acrylic acid) (HMPAA) have been studied and compared it to the release of ibuprofen, a hydrophobic active substance. Lactose is one of the most used excipients for tablets, but lactose release has not been widely studied. One reason could be a lack of good analytical tools. A novel biosensor with cellobiose dehydrogenase (CDH) was used to detect the lactose release, which has a polydiallyldimethylammonium chloride (PDADMAC) layer that increases the response. A sample treatment using polyethylenimine (PEI) was developed to eliminate possible denaturants. The developed methodology provided a good approach to detect and quantify the released lactose. The release was studied with or without the presence of a model amphiphilic substance, sodium dodecyl sulphate (SDS), in the release medium. Ibuprofen showed very different release rates in the different media, which was attributed to hydrophobic interactions between the drug, the HMPAA and the SDS in the release medium. The release of hydrophilic lactose, which did not associate to any of the other components, was rapid and showed only minor differences. The new methodology provides a useful tool to further evaluate tablet formulations by a relatively simple set of experiments. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Journal of Pharmaceutics
volume
468
issue
1-2
pages
121 - 132
publisher
Elsevier
external identifiers
  • pmid:24726632
  • wos:000336486600014
  • scopus:84899671221
ISSN
1873-3476
DOI
10.1016/j.ijpharm.2014.03.060
language
English
LU publication?
yes
id
fc27b2b7-ddaa-49e7-bf2e-dd14d49adcf4 (old id 4430228)
date added to LUP
2014-05-07 17:52:57
date last changed
2017-09-24 03:13:07
@article{fc27b2b7-ddaa-49e7-bf2e-dd14d49adcf4,
  abstract     = {The release of lactose (hydrophilic) from polymer tablets made with hydrophobically modified poly(acrylic acid) (HMPAA) have been studied and compared it to the release of ibuprofen, a hydrophobic active substance. Lactose is one of the most used excipients for tablets, but lactose release has not been widely studied. One reason could be a lack of good analytical tools. A novel biosensor with cellobiose dehydrogenase (CDH) was used to detect the lactose release, which has a polydiallyldimethylammonium chloride (PDADMAC) layer that increases the response. A sample treatment using polyethylenimine (PEI) was developed to eliminate possible denaturants. The developed methodology provided a good approach to detect and quantify the released lactose. The release was studied with or without the presence of a model amphiphilic substance, sodium dodecyl sulphate (SDS), in the release medium. Ibuprofen showed very different release rates in the different media, which was attributed to hydrophobic interactions between the drug, the HMPAA and the SDS in the release medium. The release of hydrophilic lactose, which did not associate to any of the other components, was rapid and showed only minor differences. The new methodology provides a useful tool to further evaluate tablet formulations by a relatively simple set of experiments.},
  author       = {Knöös, Patrik and Schulz, Christopher and Piculell, Lennart and Ludwig, Roland and Gorton, Lo and Wahlgren, Marie},
  issn         = {1873-3476},
  language     = {eng},
  number       = {1-2},
  pages        = {121--132},
  publisher    = {Elsevier},
  series       = {International Journal of Pharmaceutics},
  title        = {Quantifying the release of lactose from polymer matrix tablets with an amperometric biosensor utilizing cellobiose dehydrogenase.},
  url          = {http://dx.doi.org/10.1016/j.ijpharm.2014.03.060},
  volume       = {468},
  year         = {2014},
}