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AKT and AMPK Activation after High-Fat and High-Glucose In Vitro Treatment of Prostate Epithelial Cells.

Ribeiro, D L; Góes, R M; Pinto-Fochi, M E; Taboga, S R; Abrahamsson, Per-Anders LU and Dizeyi, Nishtman LU (2014) In Hormone and Metabolic Research 46(7). p.471-476
Abstract
Considering the increasing consumption of saturated fat and glucose in diets worldwide and its possible association to carcinogenesis, this investigation analysed the proliferation profile of nonmalignant human prostate epithelial cells after exposure to elevated levels of fat and glucose. PNT1A cells were cultured with palmitate (100 or 200 μM) and/or glucose (450 mg/dl) for 24 or 48 h. Treated cells were evaluated for viability test and cell proliferation (MTS assay). AKT and AMPK phosphorylation status were analysed by Western blotting. After 24 h of high-fat alone or associated with high-glucose treatment, there was an increase in AMPK and AKT activation associated to unchanged MTS-cell proliferation. Following 48 h of high-fat but not... (More)
Considering the increasing consumption of saturated fat and glucose in diets worldwide and its possible association to carcinogenesis, this investigation analysed the proliferation profile of nonmalignant human prostate epithelial cells after exposure to elevated levels of fat and glucose. PNT1A cells were cultured with palmitate (100 or 200 μM) and/or glucose (450 mg/dl) for 24 or 48 h. Treated cells were evaluated for viability test and cell proliferation (MTS assay). AKT and AMPK phosphorylation status were analysed by Western blotting. After 24 h of high-fat alone or associated with high-glucose treatment, there was an increase in AMPK and AKT activation associated to unchanged MTS-cell proliferation. Following 48 h of high-fat but not high-glucose alone, cells decreased AMPK activation and maintained elevated AKT levels. These data were associated to increased cell proliferation after further high-fat treatment. After longer high-fat exposure, MTS revealed that cells remained proliferating. High-glucose alone or associated to high-fat treatment was not able to increase cell proliferation and AKT activation. A high-fat medium containing 100 μM of palmitate stimulates proliferation in PNT1A cells by decreasing the activation of AMPK and increasing activation of AKT after longer exposure time. These findings improve the knowledge about the negative effect of high levels of this saturated fatty acid on proliferative disorders of prostate. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Hormone and Metabolic Research
volume
46
issue
7
pages
471 - 476
publisher
Georg Thieme Verlag
external identifiers
  • pmid:24799027
  • wos:000337172800003
  • scopus:84902532972
ISSN
1439-4286
DOI
10.1055/s-0034-1370991
language
English
LU publication?
yes
id
ecfadb78-d301-4405-b1b1-8ffa1e75a60d (old id 4455756)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24799027?dopt=Abstract
date added to LUP
2014-06-03 21:01:56
date last changed
2017-07-02 03:26:48
@article{ecfadb78-d301-4405-b1b1-8ffa1e75a60d,
  abstract     = {Considering the increasing consumption of saturated fat and glucose in diets worldwide and its possible association to carcinogenesis, this investigation analysed the proliferation profile of nonmalignant human prostate epithelial cells after exposure to elevated levels of fat and glucose. PNT1A cells were cultured with palmitate (100 or 200 μM) and/or glucose (450 mg/dl) for 24 or 48 h. Treated cells were evaluated for viability test and cell proliferation (MTS assay). AKT and AMPK phosphorylation status were analysed by Western blotting. After 24 h of high-fat alone or associated with high-glucose treatment, there was an increase in AMPK and AKT activation associated to unchanged MTS-cell proliferation. Following 48 h of high-fat but not high-glucose alone, cells decreased AMPK activation and maintained elevated AKT levels. These data were associated to increased cell proliferation after further high-fat treatment. After longer high-fat exposure, MTS revealed that cells remained proliferating. High-glucose alone or associated to high-fat treatment was not able to increase cell proliferation and AKT activation. A high-fat medium containing 100 μM of palmitate stimulates proliferation in PNT1A cells by decreasing the activation of AMPK and increasing activation of AKT after longer exposure time. These findings improve the knowledge about the negative effect of high levels of this saturated fatty acid on proliferative disorders of prostate.},
  author       = {Ribeiro, D L and Góes, R M and Pinto-Fochi, M E and Taboga, S R and Abrahamsson, Per-Anders and Dizeyi, Nishtman},
  issn         = {1439-4286},
  language     = {eng},
  number       = {7},
  pages        = {471--476},
  publisher    = {Georg Thieme Verlag},
  series       = {Hormone and Metabolic Research},
  title        = {AKT and AMPK Activation after High-Fat and High-Glucose In Vitro Treatment of Prostate Epithelial Cells.},
  url          = {http://dx.doi.org/10.1055/s-0034-1370991},
  volume       = {46},
  year         = {2014},
}