Ultrahigh-pressure supercritical fluid extraction and chromatography of Moringa oleifera and Moringa peregrina seed lipids
(2019) In Analytical and Bioanalytical Chemistry 411(16). p.3685-3693- Abstract
An ultrahigh-pressure supercritical fluid extraction method was optimized and applied to extract seed oil lipids from two moringa species, namely Moringa oleifera (MO) and Moringa peregrina (MP). A full-factorial design was used to investigate the direct and interaction influence of pressure and temperature in the range of 40 to 80 MPa and 40 to 70 °C, respectively, on the extracted amount of oil from crushed seeds. The results revealed that pressure has a significant positive influence on the extracted amount of oil. The best extraction condition using neat CO
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An ultrahigh-pressure supercritical fluid extraction method was optimized and applied to extract seed oil lipids from two moringa species, namely Moringa oleifera (MO) and Moringa peregrina (MP). A full-factorial design was used to investigate the direct and interaction influence of pressure and temperature in the range of 40 to 80 MPa and 40 to 70 °C, respectively, on the extracted amount of oil from crushed seeds. The results revealed that pressure has a significant positive influence on the extracted amount of oil. The best extraction condition using neat CO
2
was found at 80 MPa and 57 °C, yielding 396 ± 23 and 529 ± 26 mg oil per gram of seeds for MO and MP, respectively. An extraction kinetics study revealed a mainly solubility-controlled extraction of oil, and 28 g of CO
2
was required to extract 400 mg of oil per gram of seeds of MO using the developed method. Addition of ethanol to the sample prior to the extraction increased the proportion of extractable polar lipids as well as the total amount of extracted oil. The developed method increased the extracted amount of oil twofold compared to a reference method based on solvent sonication. The obtained oil consisted mainly of glycerolipids, sterol esters, and phospholipids. Phospholipids, campesterol, and stigmasterol ester concentrations were found to be higher in MO while cholesterol ester was more abundant in MP.
- author
- Belo, Yannick Nuapia ; Al-Hamimi, Said LU ; Chimuka, Luke LU and Turner, Charlotta LU
- organization
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Lipid profiling, Moringa oleifera, Moringa peregrina, Phospholipids, SFE, Sonication, Ultrahigh-pressure supercritical fluid extraction
- in
- Analytical and Bioanalytical Chemistry
- volume
- 411
- issue
- 16
- pages
- 3685 - 3693
- publisher
- Springer
- external identifiers
-
- scopus:85065287383
- pmid:31053955
- ISSN
- 1618-2642
- DOI
- 10.1007/s00216-019-01850-x
- language
- English
- LU publication?
- yes
- id
- 447c4cfe-12db-4789-acc7-19e8e7298b08
- date added to LUP
- 2019-05-24 12:02:19
- date last changed
- 2024-09-17 22:13:22
@article{447c4cfe-12db-4789-acc7-19e8e7298b08, abstract = {{<p><br> An ultrahigh-pressure supercritical fluid extraction method was optimized and applied to extract seed oil lipids from two moringa species, namely Moringa oleifera (MO) and Moringa peregrina (MP). A full-factorial design was used to investigate the direct and interaction influence of pressure and temperature in the range of 40 to 80 MPa and 40 to 70 °C, respectively, on the extracted amount of oil from crushed seeds. The results revealed that pressure has a significant positive influence on the extracted amount of oil. The best extraction condition using neat CO <br> <sub>2</sub><br> was found at 80 MPa and 57 °C, yielding 396 ± 23 and 529 ± 26 mg oil per gram of seeds for MO and MP, respectively. An extraction kinetics study revealed a mainly solubility-controlled extraction of oil, and 28 g of CO <br> <sub>2</sub><br> was required to extract 400 mg of oil per gram of seeds of MO using the developed method. Addition of ethanol to the sample prior to the extraction increased the proportion of extractable polar lipids as well as the total amount of extracted oil. The developed method increased the extracted amount of oil twofold compared to a reference method based on solvent sonication. The obtained oil consisted mainly of glycerolipids, sterol esters, and phospholipids. Phospholipids, campesterol, and stigmasterol ester concentrations were found to be higher in MO while cholesterol ester was more abundant in MP. <br> </p>}}, author = {{Belo, Yannick Nuapia and Al-Hamimi, Said and Chimuka, Luke and Turner, Charlotta}}, issn = {{1618-2642}}, keywords = {{Lipid profiling; Moringa oleifera; Moringa peregrina; Phospholipids; SFE; Sonication; Ultrahigh-pressure supercritical fluid extraction}}, language = {{eng}}, number = {{16}}, pages = {{3685--3693}}, publisher = {{Springer}}, series = {{Analytical and Bioanalytical Chemistry}}, title = {{Ultrahigh-pressure supercritical fluid extraction and chromatography of Moringa oleifera and Moringa peregrina seed lipids}}, url = {{http://dx.doi.org/10.1007/s00216-019-01850-x}}, doi = {{10.1007/s00216-019-01850-x}}, volume = {{411}}, year = {{2019}}, }