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Thermostable glycoside hydrolases in Biorefining

Linares-Pastén, Javier LU ; Andersson, Maria LU and Nordberg Karlsson, Eva LU (2014) In Current Biotechnology 3(1). p.26-44
Abstract
Glycoside hydrolases, which are responsible for the degradation of the major fraction of biomass, the polymeric carbohydrates in starch and lignocellulose, are predicted to gain increasing roles as catalysts in biorefining applications in the future bioeconomy. In this context, thermostable variants will be important, as the recalcitrance of these biomass-components to degradation often motivates thermal treatments. The traditional focus on degradation is also predicted to be changed into more versatile roles of the enzymes, also involving specific conversions to defined products. In addition, integration of genes encoding interesting target activities opens the possibilities for whole cell applications, in organisms allowing processing at... (More)
Glycoside hydrolases, which are responsible for the degradation of the major fraction of biomass, the polymeric carbohydrates in starch and lignocellulose, are predicted to gain increasing roles as catalysts in biorefining applications in the future bioeconomy. In this context, thermostable variants will be important, as the recalcitrance of these biomass-components to degradation often motivates thermal treatments. The traditional focus on degradation is also predicted to be changed into more versatile roles of the enzymes, also involving specific conversions to defined products. In addition, integration of genes encoding interesting target activities opens the possibilities for whole cell applications, in organisms allowing processing at elevated temperatures for production of defined metabolic products.

In this review, we overview the application of glycoside hydrolases related to the biorefining context (for production of food, chemicals, and fuels). Use of thermostable enzymes in processing of biomass is highlighted, moving from the activities required to act on different types of polymers, to specific examples in today’s processing. Examples given involve (i) monosaccharide production for food applications as well as use as carbon source for microbial conversions (to metabolites such as fuels and chemical intermediates), (ii) oligosaccharide production for prebiotics applications (iii) treatment for plant metabolite product release, and (iv) production of surfactants of the alkyl glycoside class. Finally future possibilities in whole cell biorefining are shown. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
xylanase, xylan, surfactant, starch, prebiotics, pectin, mannan, lignocellulose, hemicellulose, glucosidase, glucanase, cellulase, biomass, biofuels, antioxidant, amylase
in
Current Biotechnology
volume
3
issue
1
pages
26 - 44
publisher
Bentham Science Publishers
ISSN
2211-5501
DOI
10.2174/22115501113026660041
language
English
LU publication?
yes
id
fe8a80c9-4164-4751-ae43-4d263649fcdb (old id 4499462)
date added to LUP
2014-07-03 12:17:52
date last changed
2016-09-30 05:33:26
@article{fe8a80c9-4164-4751-ae43-4d263649fcdb,
  abstract     = {Glycoside hydrolases, which are responsible for the degradation of the major fraction of biomass, the polymeric carbohydrates in starch and lignocellulose, are predicted to gain increasing roles as catalysts in biorefining applications in the future bioeconomy. In this context, thermostable variants will be important, as the recalcitrance of these biomass-components to degradation often motivates thermal treatments. The traditional focus on degradation is also predicted to be changed into more versatile roles of the enzymes, also involving specific conversions to defined products. In addition, integration of genes encoding interesting target activities opens the possibilities for whole cell applications, in organisms allowing processing at elevated temperatures for production of defined metabolic products. <br/><br>
In this review, we overview the application of glycoside hydrolases related to the biorefining context (for production of food, chemicals, and fuels). Use of thermostable enzymes in processing of biomass is highlighted, moving from the activities required to act on different types of polymers, to specific examples in today’s processing. Examples given involve (i) monosaccharide production for food applications as well as use as carbon source for microbial conversions (to metabolites such as fuels and chemical intermediates), (ii) oligosaccharide production for prebiotics applications (iii) treatment for plant metabolite product release, and (iv) production of surfactants of the alkyl glycoside class. Finally future possibilities in whole cell biorefining are shown.},
  author       = {Linares-Pastén, Javier and Andersson, Maria and Nordberg Karlsson, Eva},
  issn         = {2211-5501},
  keyword      = {xylanase,xylan,surfactant,starch,prebiotics,pectin,mannan,lignocellulose,hemicellulose,glucosidase,glucanase,cellulase,biomass,biofuels,antioxidant,amylase},
  language     = {eng},
  number       = {1},
  pages        = {26--44},
  publisher    = {Bentham Science Publishers},
  series       = {Current Biotechnology},
  title        = {Thermostable glycoside hydrolases in Biorefining},
  url          = {http://dx.doi.org/10.2174/22115501113026660041},
  volume       = {3},
  year         = {2014},
}