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Fluid and Highly Curved Model Membranes on Vertical Nanowire Arrays

Dabkowska, Aleksandra LU ; Niman, Cassandra LU ; Piret, Gaelle LU ; Persson, Henrik LU ; Wacklin, Hanna LU ; Linke, Heiner LU ; Prinz, Christelle LU and Nylander, Tommy LU (2014) In Nano Letters 14(8). p.4286-4292
Abstract
Sensing and manipulating living cells using vertical nanowire devices requires a complete understanding of cell behavior on these substrates. Changes in cell function and phenotype are often triggered by events taking place at the plasma membrane, the properties of which are influenced by local curvature. The nanowire topography can therefore be expected to greatly affect the cell membrane, emphasizing the importance of studying membranes on vertical nanowire arrays. Here, we used supported phospholipid bilayers as a model for biomembranes. We demonstrate the formation of fluid supported bilayers on vertical nanowire forests using self-assembly from vesicles in solution. The bilayers were found to follow the contours of the nanowires to... (More)
Sensing and manipulating living cells using vertical nanowire devices requires a complete understanding of cell behavior on these substrates. Changes in cell function and phenotype are often triggered by events taking place at the plasma membrane, the properties of which are influenced by local curvature. The nanowire topography can therefore be expected to greatly affect the cell membrane, emphasizing the importance of studying membranes on vertical nanowire arrays. Here, we used supported phospholipid bilayers as a model for biomembranes. We demonstrate the formation of fluid supported bilayers on vertical nanowire forests using self-assembly from vesicles in solution. The bilayers were found to follow the contours of the nanowires to form continuous and locally highly curved model membranes. Distinct from standard flat supported lipid bilayers, the high aspect ratio of the nanowires results in a large bilayer surface available for the immobilization and study of biomolecules. We used these bilayers to bind a membrane-anchored protein as well as tethered vesicles on the nanowire substrate. The nanowire-bilayer platform shown here can be expanded from fundamental studies of lipid membranes on controlled curvature substrates to the development of innovative membrane-based nanosensors. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Nanowires, lipid bilayer, membrane curvature, protein-lipid interaction, nanotopography, nanostructure
in
Nano Letters
volume
14
issue
8
pages
4286 - 4292
publisher
The American Chemical Society
external identifiers
  • wos:000340446200014
  • scopus:84906091016
ISSN
1530-6992
DOI
10.1021/nl500926y
language
English
LU publication?
yes
id
56fc2bc2-f069-4e3d-b57e-dc9807e1630b (old id 4652964)
date added to LUP
2014-09-24 10:51:16
date last changed
2017-11-19 04:01:23
@article{56fc2bc2-f069-4e3d-b57e-dc9807e1630b,
  abstract     = {Sensing and manipulating living cells using vertical nanowire devices requires a complete understanding of cell behavior on these substrates. Changes in cell function and phenotype are often triggered by events taking place at the plasma membrane, the properties of which are influenced by local curvature. The nanowire topography can therefore be expected to greatly affect the cell membrane, emphasizing the importance of studying membranes on vertical nanowire arrays. Here, we used supported phospholipid bilayers as a model for biomembranes. We demonstrate the formation of fluid supported bilayers on vertical nanowire forests using self-assembly from vesicles in solution. The bilayers were found to follow the contours of the nanowires to form continuous and locally highly curved model membranes. Distinct from standard flat supported lipid bilayers, the high aspect ratio of the nanowires results in a large bilayer surface available for the immobilization and study of biomolecules. We used these bilayers to bind a membrane-anchored protein as well as tethered vesicles on the nanowire substrate. The nanowire-bilayer platform shown here can be expanded from fundamental studies of lipid membranes on controlled curvature substrates to the development of innovative membrane-based nanosensors.},
  author       = {Dabkowska, Aleksandra and Niman, Cassandra and Piret, Gaelle and Persson, Henrik and Wacklin, Hanna and Linke, Heiner and Prinz, Christelle and Nylander, Tommy},
  issn         = {1530-6992},
  keyword      = {Nanowires,lipid bilayer,membrane curvature,protein-lipid interaction,nanotopography,nanostructure},
  language     = {eng},
  number       = {8},
  pages        = {4286--4292},
  publisher    = {The American Chemical Society},
  series       = {Nano Letters},
  title        = {Fluid and Highly Curved Model Membranes on Vertical Nanowire Arrays},
  url          = {http://dx.doi.org/10.1021/nl500926y},
  volume       = {14},
  year         = {2014},
}