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Digital holographic microscopy for non-invasive monitoring of cell cycle arrest in l929 cells.

Falck Miniotis, Maria LU ; Mukwaya, Anthonny LU and Gjörloff Wingren, Anette LU (2014) In PLoS ONE 9(9).
Abstract
Digital holographic microscopy (DHM) has emerged as a powerful non-invasive tool for cell analysis. It has the capacity to analyse multiple parameters simultaneously, such as cell- number, confluence and phase volume. This is done while cells are still adhered and growing in their culture flask. The aim of this study was to investigate whether DHM was able to monitor drug-induced cell cycle arrest in cultured cells and thus provide a non-disruptive alternative to flow cytometry. DHM parameters from G1 and G2/M cell cycle arrested L929 mouse fibroblast cells were collected. Cell cycle arrest was verified with flow cytometry. This study shows that DHM is able to monitor phase volume changes corresponding to either a G1 or G2/M cell cycle... (More)
Digital holographic microscopy (DHM) has emerged as a powerful non-invasive tool for cell analysis. It has the capacity to analyse multiple parameters simultaneously, such as cell- number, confluence and phase volume. This is done while cells are still adhered and growing in their culture flask. The aim of this study was to investigate whether DHM was able to monitor drug-induced cell cycle arrest in cultured cells and thus provide a non-disruptive alternative to flow cytometry. DHM parameters from G1 and G2/M cell cycle arrested L929 mouse fibroblast cells were collected. Cell cycle arrest was verified with flow cytometry. This study shows that DHM is able to monitor phase volume changes corresponding to either a G1 or G2/M cell cycle arrest. G1-phase arrest with staurosporine correlated with a decrease in the average cell phase volume and G2/M-phase arrest with colcemid and etoposide correlated with an increase in the average cell phase volume. Importantly, DHM analysis of average cell phase volume was of comparable accuracy to flow cytometric measurement of cell cycle phase distribution as recorded following dose-dependent treatment with etoposide. Average cell phase volume changes in response to treatment with cell cycle arresting compounds could therefore be used as a DHM marker for monitoring cell cycle arrest in cultured mammalian cells. (Less)
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publication status
published
subject
in
PLoS ONE
volume
9
issue
9
article number
e106546
publisher
Public Library of Science (PLoS)
external identifiers
  • pmid:25208094
  • wos:000342030300028
  • pmid:25208094
  • scopus:84930067743
ISSN
1932-6203
DOI
10.1371/journal.pone.0106546
language
English
LU publication?
yes
additional info
Department affilation moved from v1000588 (Tumour Biology, Malmö) to v1000562 (Department of Translational Medicine) on 2016-01-18 14:39:26.
id
309dd87b-f448-497c-a86b-ff7dcdb1eafa (old id 4691921)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25208094?dopt=Abstract
date added to LUP
2016-04-01 14:44:43
date last changed
2021-09-01 02:40:19
@article{309dd87b-f448-497c-a86b-ff7dcdb1eafa,
  abstract     = {Digital holographic microscopy (DHM) has emerged as a powerful non-invasive tool for cell analysis. It has the capacity to analyse multiple parameters simultaneously, such as cell- number, confluence and phase volume. This is done while cells are still adhered and growing in their culture flask. The aim of this study was to investigate whether DHM was able to monitor drug-induced cell cycle arrest in cultured cells and thus provide a non-disruptive alternative to flow cytometry. DHM parameters from G1 and G2/M cell cycle arrested L929 mouse fibroblast cells were collected. Cell cycle arrest was verified with flow cytometry. This study shows that DHM is able to monitor phase volume changes corresponding to either a G1 or G2/M cell cycle arrest. G1-phase arrest with staurosporine correlated with a decrease in the average cell phase volume and G2/M-phase arrest with colcemid and etoposide correlated with an increase in the average cell phase volume. Importantly, DHM analysis of average cell phase volume was of comparable accuracy to flow cytometric measurement of cell cycle phase distribution as recorded following dose-dependent treatment with etoposide. Average cell phase volume changes in response to treatment with cell cycle arresting compounds could therefore be used as a DHM marker for monitoring cell cycle arrest in cultured mammalian cells.},
  author       = {Falck Miniotis, Maria and Mukwaya, Anthonny and Gjörloff Wingren, Anette},
  issn         = {1932-6203},
  language     = {eng},
  number       = {9},
  publisher    = {Public Library of Science (PLoS)},
  series       = {PLoS ONE},
  title        = {Digital holographic microscopy for non-invasive monitoring of cell cycle arrest in l929 cells.},
  url          = {https://lup.lub.lu.se/search/files/4140179/5310400},
  doi          = {10.1371/journal.pone.0106546},
  volume       = {9},
  year         = {2014},
}