High salt buffer improves integrity of RNA after fluorescence-activated cell sorting of intracellular labeled cells.
Nilsson, Helén LU ; Krawczyk, Krzysztof LU
and Johansson, Martin E
(2014)
In Journal of Biotechnology
192(Sep 30).
p.62-65
- Abstract
- Over the past years, massive progress has been made in the ability to collect large-scale gene expression data from a limited sample size. Combined with improvements in multiplex flow cytometry-based techniques, this has made it possible to isolate and characterize specific cellular subtypes within heterogeneous populations, with a great impact on our understanding of different biological processes. However, sorting based on intracellular markers requires fixation and permeabilization of samples, and very often the integrity of RNA molecules is compromised during this process. Many attempts have been made to improve the quality of nucleic acids from such samples, but RNA degradation still remains a limiting factor for downstream analyses.... (More)
- Over the past years, massive progress has been made in the ability to collect large-scale gene expression data from a limited sample size. Combined with improvements in multiplex flow cytometry-based techniques, this has made it possible to isolate and characterize specific cellular subtypes within heterogeneous populations, with a great impact on our understanding of different biological processes. However, sorting based on intracellular markers requires fixation and permeabilization of samples, and very often the integrity of RNA molecules is compromised during this process. Many attempts have been made to improve the quality of nucleic acids from such samples, but RNA degradation still remains a limiting factor for downstream analyses. Here we present a method to isolate high quality RNA from cells that have been fixed, permeabilized, intracellularly labeled and sorted. By performing all incubation steps in the presence of a high salt buffer, RNA degradation was avoided and samples with remarkable integrity were obtained. This procedure offers a straightforward and very affordable technique to retrieve high quality RNA from isolated cell populations, which increases the possibilities to characterize gene expression profiles of subpopulations from mixed samples, a technique with implications in a broad range of research fields. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4738497
- author
- Nilsson, Helén
LU
; Krawczyk, Krzysztof
LU
and Johansson, Martin E
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biotechnology
- volume
- 192
- issue
- Sep 30
- pages
- 62 - 65
- publisher
- Elsevier
- external identifiers
-
- pmid:25277986
- wos:000345970300011
- scopus:84909635513
- ISSN
- 1873-4863
- DOI
- 10.1016/j.jbiotec.2014.09.016
- language
- English
- LU publication?
- yes
- id
- a3c5cd62-a617-4295-a50c-5ab276b4b694 (old id 4738497)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/25277986?dopt=Abstract
- date added to LUP
- 2016-04-01 10:00:19
- date last changed
- 2022-02-17 05:45:13
@article{a3c5cd62-a617-4295-a50c-5ab276b4b694,
abstract = {{Over the past years, massive progress has been made in the ability to collect large-scale gene expression data from a limited sample size. Combined with improvements in multiplex flow cytometry-based techniques, this has made it possible to isolate and characterize specific cellular subtypes within heterogeneous populations, with a great impact on our understanding of different biological processes. However, sorting based on intracellular markers requires fixation and permeabilization of samples, and very often the integrity of RNA molecules is compromised during this process. Many attempts have been made to improve the quality of nucleic acids from such samples, but RNA degradation still remains a limiting factor for downstream analyses. Here we present a method to isolate high quality RNA from cells that have been fixed, permeabilized, intracellularly labeled and sorted. By performing all incubation steps in the presence of a high salt buffer, RNA degradation was avoided and samples with remarkable integrity were obtained. This procedure offers a straightforward and very affordable technique to retrieve high quality RNA from isolated cell populations, which increases the possibilities to characterize gene expression profiles of subpopulations from mixed samples, a technique with implications in a broad range of research fields.}},
author = {{Nilsson, Helén and Krawczyk, Krzysztof and Johansson, Martin E}},
issn = {{1873-4863}},
language = {{eng}},
number = {{Sep 30}},
pages = {{62--65}},
publisher = {{Elsevier}},
series = {{Journal of Biotechnology}},
title = {{High salt buffer improves integrity of RNA after fluorescence-activated cell sorting of intracellular labeled cells.}},
url = {{https://lup.lub.lu.se/search/files/1473921/5424363}},
doi = {{10.1016/j.jbiotec.2014.09.016}},
volume = {{192}},
year = {{2014}},
}