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Inhibition of Ras signalling reduces neutrophil infiltration and tissue damage in severe acute pancreatitis.

Changhui, Yu LU ; Merza, Mohammed LU ; Luo, Lingtao and Thorlacius, Henrik LU (2015) In European Journal of Pharmacology 746. p.245-251
Abstract
Neutrophil recruitment is known to be a rate-limiting step in mediating tissue injury in severe acute pancreatitis (AP). However, the signalling mechanisms controlling inflammation and organ damage in AP remain elusive. Herein, we examined the role of Ras signalling in AP. Male C57BL/6 mice were treated with a Ras inhibitor (farnesylthiosalicylic acid, FTS) before infusion of taurocholate into the pancreatic duct. Pancreatic and lung tissues as well as blood were collected 24h after pancreatitis induction. Pretreatment with FTS decreased serum amylase levels by 82% and significantly attenuated acinar cell necrosis, tissue haemorrhage and oedema formation in taurocholate-induced pancreatitis. Inhibition of Ras signalling reduced... (More)
Neutrophil recruitment is known to be a rate-limiting step in mediating tissue injury in severe acute pancreatitis (AP). However, the signalling mechanisms controlling inflammation and organ damage in AP remain elusive. Herein, we examined the role of Ras signalling in AP. Male C57BL/6 mice were treated with a Ras inhibitor (farnesylthiosalicylic acid, FTS) before infusion of taurocholate into the pancreatic duct. Pancreatic and lung tissues as well as blood were collected 24h after pancreatitis induction. Pretreatment with FTS decreased serum amylase levels by 82% and significantly attenuated acinar cell necrosis, tissue haemorrhage and oedema formation in taurocholate-induced pancreatitis. Inhibition of Ras signalling reduced myeloperoxidase (MPO) levels in the inflamed pancreas by 42%. In addition, administration of FTS decreased pancreatic levels of CXC chemokines as well as circulating levels of interleukin-6 and high-mobility group box 1 in animals exposed to taurocholate. Moreover, treatment with FTS reduced taurocholate-induced MPO levels in the lung. Inhibition of Ras signalling had no effect on neutrophil expression of Mac-1 in mice with pancreatitis. Moreover, FTS had no direct impact on trypsin activation in isolated pancreatic acinar cells. These results indicate that Ras signalling controls CXC chemokine formation, neutrophil recruitment and tissue injury in severe AP. Thus, our findings highlight a new signalling mechanism regulating neutrophil recruitment in the pancreas and suggest that inhibition of Ras signalling might be a useful strategy to attenuate local and systemic inflammation in severe AP. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
European Journal of Pharmacology
volume
746
pages
245 - 251
publisher
Elsevier
external identifiers
  • pmid:25460024
  • wos:000346854000030
  • scopus:84918562568
ISSN
1879-0712
DOI
10.1016/j.ejphar.2014.11.020
language
English
LU publication?
yes
id
3d621bc2-897b-4c17-a1ae-142fff39045d (old id 4913043)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25460024?dopt=Abstract
date added to LUP
2015-01-08 17:14:58
date last changed
2017-09-10 03:17:51
@article{3d621bc2-897b-4c17-a1ae-142fff39045d,
  abstract     = {Neutrophil recruitment is known to be a rate-limiting step in mediating tissue injury in severe acute pancreatitis (AP). However, the signalling mechanisms controlling inflammation and organ damage in AP remain elusive. Herein, we examined the role of Ras signalling in AP. Male C57BL/6 mice were treated with a Ras inhibitor (farnesylthiosalicylic acid, FTS) before infusion of taurocholate into the pancreatic duct. Pancreatic and lung tissues as well as blood were collected 24h after pancreatitis induction. Pretreatment with FTS decreased serum amylase levels by 82% and significantly attenuated acinar cell necrosis, tissue haemorrhage and oedema formation in taurocholate-induced pancreatitis. Inhibition of Ras signalling reduced myeloperoxidase (MPO) levels in the inflamed pancreas by 42%. In addition, administration of FTS decreased pancreatic levels of CXC chemokines as well as circulating levels of interleukin-6 and high-mobility group box 1 in animals exposed to taurocholate. Moreover, treatment with FTS reduced taurocholate-induced MPO levels in the lung. Inhibition of Ras signalling had no effect on neutrophil expression of Mac-1 in mice with pancreatitis. Moreover, FTS had no direct impact on trypsin activation in isolated pancreatic acinar cells. These results indicate that Ras signalling controls CXC chemokine formation, neutrophil recruitment and tissue injury in severe AP. Thus, our findings highlight a new signalling mechanism regulating neutrophil recruitment in the pancreas and suggest that inhibition of Ras signalling might be a useful strategy to attenuate local and systemic inflammation in severe AP.},
  author       = {Changhui, Yu and Merza, Mohammed and Luo, Lingtao and Thorlacius, Henrik},
  issn         = {1879-0712},
  language     = {eng},
  pages        = {245--251},
  publisher    = {Elsevier},
  series       = {European Journal of Pharmacology},
  title        = {Inhibition of Ras signalling reduces neutrophil infiltration and tissue damage in severe acute pancreatitis.},
  url          = {http://dx.doi.org/10.1016/j.ejphar.2014.11.020},
  volume       = {746},
  year         = {2015},
}