Advanced

Identification and characterization of high protein oat lines from a mutagenized oat population

Sunilkumar, Bindu A. LU ; Leonova, Svetlana LU ; Öste, Rickard LU and Olsson, Olof LU (2017) In Journal of Cereal Science 75. p.100-107
Abstract

Oat is an excellent source of high-quality proteins. The protein content in cultivated oat is about 12%–15%. Oats with even higher protein contents would have great potential as a vegetative protein source. From a mutagenized oat population, 230 lines with seed protein levels of 15% or higher were identified from green house grown plants. Fifteen lines with protein levels ranging between 17 and 24% were chosen for further studies. The high protein character was confirmed by two consecutive propagations in the field. Crossings showed that the trait was stable and segregated in a Mendelian fashion. SDS-PAGE analysis revealed that the major increase was in globulin like proteins. In addition, total dietary fibre, β-glucan and lipid levels... (More)

Oat is an excellent source of high-quality proteins. The protein content in cultivated oat is about 12%–15%. Oats with even higher protein contents would have great potential as a vegetative protein source. From a mutagenized oat population, 230 lines with seed protein levels of 15% or higher were identified from green house grown plants. Fifteen lines with protein levels ranging between 17 and 24% were chosen for further studies. The high protein character was confirmed by two consecutive propagations in the field. Crossings showed that the trait was stable and segregated in a Mendelian fashion. SDS-PAGE analysis revealed that the major increase was in globulin like proteins. In addition, total dietary fibre, β-glucan and lipid levels were quantified. No correlation between any of these characters and the high protein trait could be found. Thousand Kernel Weight was also monitored and shown to be stable in the high protein lines. Thus, starting from the lines presented here a further development of commercial oat varieties with protein levels of 20% or higher is feasible. Such a high protein oat would be very important to meet the consumers need for high-quality high-protein oat for the food and feed industry.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Beta glucan, Crossing, High protein, Mutagenized population, Oats, Screening
in
Journal of Cereal Science
volume
75
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:85016800093
  • wos:000404792200014
ISSN
0733-5210
DOI
10.1016/j.jcs.2017.03.003
language
English
LU publication?
yes
id
49777e24-f129-41b2-a4e7-c442926f9846
date added to LUP
2017-04-26 13:27:15
date last changed
2017-09-18 13:33:16
@article{49777e24-f129-41b2-a4e7-c442926f9846,
  abstract     = {<p>Oat is an excellent source of high-quality proteins. The protein content in cultivated oat is about 12%–15%. Oats with even higher protein contents would have great potential as a vegetative protein source. From a mutagenized oat population, 230 lines with seed protein levels of 15% or higher were identified from green house grown plants. Fifteen lines with protein levels ranging between 17 and 24% were chosen for further studies. The high protein character was confirmed by two consecutive propagations in the field. Crossings showed that the trait was stable and segregated in a Mendelian fashion. SDS-PAGE analysis revealed that the major increase was in globulin like proteins. In addition, total dietary fibre, β-glucan and lipid levels were quantified. No correlation between any of these characters and the high protein trait could be found. Thousand Kernel Weight was also monitored and shown to be stable in the high protein lines. Thus, starting from the lines presented here a further development of commercial oat varieties with protein levels of 20% or higher is feasible. Such a high protein oat would be very important to meet the consumers need for high-quality high-protein oat for the food and feed industry.</p>},
  author       = {Sunilkumar, Bindu A. and Leonova, Svetlana and Öste, Rickard and Olsson, Olof},
  issn         = {0733-5210},
  keyword      = {Beta glucan,Crossing,High protein,Mutagenized population,Oats,Screening},
  language     = {eng},
  month        = {05},
  pages        = {100--107},
  publisher    = {Elsevier},
  series       = {Journal of Cereal Science},
  title        = {Identification and characterization of high protein oat lines from a mutagenized oat population},
  url          = {http://dx.doi.org/10.1016/j.jcs.2017.03.003},
  volume       = {75},
  year         = {2017},
}