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Stable isotopes as an indicator of diet in omnivorous crayfish (Pacifastacus leniusculus): the influence of tissue, sample treatment, and season

Stenroth, Patrik LU ; Holmqvist, Niklas LU ; Nyström, Per LU ; Berglund, Olof LU ; Larsson, Per LU and Granéli, Wilhelm LU (2006) In Canadian Journal of Fisheries and Aquatic Sciences 63(4). p.821-831
Abstract
Stable isotopes have been used to analyse food webs and (or) trace movements of animals for about 30 years. There has been some debate on the use of different tissues and treatments before isotope analysis, as well as on seasonal effects. We found different crayfish (Pacifastacus leniusculus) tissues (muscle, hepatopancreas, exoskeleton, gill, and whole body) to have different isotope values. Lipid extraction made whole-body carbon isotope values higher but had no effect on nitrogen isotope values. Acidification made whole-body isotope values lower. For crayfish, there was no seasonal or interannual variation in isotope values. In contrast to studies based on gut content analysis, we found adult crayfish to be at least as carnivorous as... (More)
Stable isotopes have been used to analyse food webs and (or) trace movements of animals for about 30 years. There has been some debate on the use of different tissues and treatments before isotope analysis, as well as on seasonal effects. We found different crayfish (Pacifastacus leniusculus) tissues (muscle, hepatopancreas, exoskeleton, gill, and whole body) to have different isotope values. Lipid extraction made whole-body carbon isotope values higher but had no effect on nitrogen isotope values. Acidification made whole-body isotope values lower. For crayfish, there was no seasonal or interannual variation in isotope values. In contrast to studies based on gut content analysis, we found adult crayfish to be at least as carnivorous as young-of-the-year crayfish. Earlier studies often have assumed that each food source contributes both nitrogen and carbon in equal proportions. Omnivores do not fit easily into this view. We suggest that nitrogen and carbon in an organism could come from different sources. Adopting this view for a pond food web could render crayfish both predators and detritivores as crayfish prey on nitrogen sources (other invertebrates) and consume large amounts of detritus to satisfy their carbon demand. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Canadian Journal of Fisheries and Aquatic Sciences
volume
63
issue
4
pages
821 - 831
publisher
National Research Council Canada
external identifiers
  • wos:000237201300010
  • scopus:33744494559
ISSN
1205-7533
DOI
10.1139/F05-265
language
English
LU publication?
yes
id
4a266aa6-d0e9-447c-83f7-1db15b8b8862 (old id 159533)
date added to LUP
2007-06-27 12:41:40
date last changed
2019-03-05 01:48:49
@article{4a266aa6-d0e9-447c-83f7-1db15b8b8862,
  abstract     = {Stable isotopes have been used to analyse food webs and (or) trace movements of animals for about 30 years. There has been some debate on the use of different tissues and treatments before isotope analysis, as well as on seasonal effects. We found different crayfish (Pacifastacus leniusculus) tissues (muscle, hepatopancreas, exoskeleton, gill, and whole body) to have different isotope values. Lipid extraction made whole-body carbon isotope values higher but had no effect on nitrogen isotope values. Acidification made whole-body isotope values lower. For crayfish, there was no seasonal or interannual variation in isotope values. In contrast to studies based on gut content analysis, we found adult crayfish to be at least as carnivorous as young-of-the-year crayfish. Earlier studies often have assumed that each food source contributes both nitrogen and carbon in equal proportions. Omnivores do not fit easily into this view. We suggest that nitrogen and carbon in an organism could come from different sources. Adopting this view for a pond food web could render crayfish both predators and detritivores as crayfish prey on nitrogen sources (other invertebrates) and consume large amounts of detritus to satisfy their carbon demand.},
  author       = {Stenroth, Patrik and Holmqvist, Niklas and Nyström, Per and Berglund, Olof and Larsson, Per and Granéli, Wilhelm},
  issn         = {1205-7533},
  language     = {eng},
  number       = {4},
  pages        = {821--831},
  publisher    = {National Research Council Canada},
  series       = {Canadian Journal of Fisheries and Aquatic Sciences},
  title        = {Stable isotopes as an indicator of diet in omnivorous crayfish (Pacifastacus leniusculus): the influence of tissue, sample treatment, and season},
  url          = {http://dx.doi.org/10.1139/F05-265},
  volume       = {63},
  year         = {2006},
}