Quality of up to 35 years old archival breast cancer tissue in paraffinblocks for estrogen receptor evaluation
(2017) 29th European Congress of Pathology In Virchows Archiv 471(Suppl. 1). p.54-54- Abstract
- Objective: Estrogen receptor (ER) positive breast cancer (BC) can have
an insidious course with disease-relapse decades after primary surgery.
New analysis performed on archived formalin-fixed paraffin-embedded(FFPE) tissue are important for disease-management in late BC-relapse
and an important tool in BC-research. However, although loss of immunoreactivity
in tissue slides after sectioning has been shown, little is
known of the preservation of biomarker-expression in FFPE tumourblocks.
We aim to investigate the quality of immunohistochemical
(IHC) ER-evaluation in FFPE-tissue over time (1978–2000).
Method: Tissue-microarrays from a Swedish multicenter cohort of 728
patients with contralateral BC was... (More) - Objective: Estrogen receptor (ER) positive breast cancer (BC) can have
an insidious course with disease-relapse decades after primary surgery.
New analysis performed on archived formalin-fixed paraffin-embedded(FFPE) tissue are important for disease-management in late BC-relapse
and an important tool in BC-research. However, although loss of immunoreactivity
in tissue slides after sectioning has been shown, little is
known of the preservation of biomarker-expression in FFPE tumourblocks.
We aim to investigate the quality of immunohistochemical
(IHC) ER-evaluation in FFPE-tissue over time (1978–2000).
Method: Tissue-microarrays from a Swedish multicenter cohort of 728
patients with contralateral BC was used for ER IHC-evaluation. BC was
studied in three periods (1958–1985, 1986–1993, 1994–2000), and retrospective
ER IHC-data was correlated to corresponding prospective ER
cytosol-analysis performed on fresh BC-tissue.
Results: The concordance between the original ER cytosol-analysis and
the new IHC was substantial (1978–1985: 82 %, (117/142), Kappa 0.52.
1986–1993: 91 %, (194/213), Kappa 0.72. 1994–2000: 86 %, (187/218),
Kappa 0.61). Discrepancies were mostly found for tumours with ERvalues
close to cutoff for one or both of the methods.
Conclusion: FFPE BC-tissue from the late 70s to millennium shows
preserved ER-antigenicity up to 35 years later. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4a6e8d8d-9ae3-48d0-9803-666bbe3c203c
- author
- Ehinger, Anna
LU
; Rydén, Lisa LU
; Bendahl, Pär-Ola LU ; Fernö, Mårten LU and Alkner, Sara LU
- organization
-
- Breastcancer-genetics
- Personalized Breast Cancer Treatment (research group)
- The Liquid Biopsy and Tumor Progression in Breast Cancer (research group)
- Breast Cancer Surgery (research group)
- Surgery (Lund)
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
- Tumor microenvironment
- publishing date
- 2017-09-02
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Virchows Archiv
- volume
- 471
- issue
- Suppl. 1
- article number
- PS-01-016
- pages
- 1 pages
- publisher
- Springer
- conference name
- 29th European Congress of Pathology
- conference location
- Amsterdam, Netherlands
- conference dates
- 2017-09-02
- ISSN
- 1432-2307
- DOI
- 10.1007/s00428-017-2205-0
- project
- Histopathological and biological markers in breast cancer – focused on methodology and contralateral tumors
- language
- English
- LU publication?
- yes
- id
- 4a6e8d8d-9ae3-48d0-9803-666bbe3c203c
- date added to LUP
- 2017-09-02 08:11:57
- date last changed
- 2021-03-23 22:43:03
@misc{4a6e8d8d-9ae3-48d0-9803-666bbe3c203c, abstract = {{Objective: Estrogen receptor (ER) positive breast cancer (BC) can have<br/>an insidious course with disease-relapse decades after primary surgery.<br/>New analysis performed on archived formalin-fixed paraffin-embedded(FFPE) tissue are important for disease-management in late BC-relapse<br/>and an important tool in BC-research. However, although loss of immunoreactivity<br/>in tissue slides after sectioning has been shown, little is<br/>known of the preservation of biomarker-expression in FFPE tumourblocks.<br/>We aim to investigate the quality of immunohistochemical<br/>(IHC) ER-evaluation in FFPE-tissue over time (1978–2000).<br/>Method: Tissue-microarrays from a Swedish multicenter cohort of 728<br/>patients with contralateral BC was used for ER IHC-evaluation. BC was<br/>studied in three periods (1958–1985, 1986–1993, 1994–2000), and retrospective<br/>ER IHC-data was correlated to corresponding prospective ER<br/>cytosol-analysis performed on fresh BC-tissue.<br/>Results: The concordance between the original ER cytosol-analysis and<br/>the new IHC was substantial (1978–1985: 82 %, (117/142), Kappa 0.52.<br/>1986–1993: 91 %, (194/213), Kappa 0.72. 1994–2000: 86 %, (187/218),<br/>Kappa 0.61). Discrepancies were mostly found for tumours with ERvalues<br/>close to cutoff for one or both of the methods.<br/>Conclusion: FFPE BC-tissue from the late 70s to millennium shows<br/>preserved ER-antigenicity up to 35 years later.}}, author = {{Ehinger, Anna and Rydén, Lisa and Bendahl, Pär-Ola and Fernö, Mårten and Alkner, Sara}}, issn = {{1432-2307}}, language = {{eng}}, month = {{09}}, note = {{Conference Abstract}}, number = {{Suppl. 1}}, pages = {{54--54}}, publisher = {{Springer}}, series = {{Virchows Archiv}}, title = {{Quality of up to 35 years old archival breast cancer tissue in paraffinblocks for estrogen receptor evaluation}}, url = {{http://dx.doi.org/10.1007/s00428-017-2205-0}}, doi = {{10.1007/s00428-017-2205-0}}, volume = {{471}}, year = {{2017}}, }