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Quality of up to 35 years old archival breast cancer tissue in paraffinblocks for estrogen receptor evaluation

Ehinger, Anna LU ; Rydén, Lisa LU ; Bendahl, Pär-Ola LU ; Fernö, Mårten LU and Alkner, Sara LU (2017) 29th European Congress of Pathology In Virchows Archiv 471(Suppl. 1). p.54-54
Abstract (Swedish)
Objective: Estrogen receptor (ER) positive breast cancer (BC) can have
an insidious course with disease-relapse decades after primary surgery.
New analysis performed on archived formalin-fixed paraffin-embedded(FFPE) tissue are important for disease-management in late BC-relapse
and an important tool in BC-research. However, although loss of immunoreactivity
in tissue slides after sectioning has been shown, little is
known of the preservation of biomarker-expression in FFPE tumourblocks.
We aim to investigate the quality of immunohistochemical
(IHC) ER-evaluation in FFPE-tissue over time (1978–2000).
Method: Tissue-microarrays from a Swedish multicenter cohort of 728
patients with contralateral BC was... (More)
Objective: Estrogen receptor (ER) positive breast cancer (BC) can have
an insidious course with disease-relapse decades after primary surgery.
New analysis performed on archived formalin-fixed paraffin-embedded(FFPE) tissue are important for disease-management in late BC-relapse
and an important tool in BC-research. However, although loss of immunoreactivity
in tissue slides after sectioning has been shown, little is
known of the preservation of biomarker-expression in FFPE tumourblocks.
We aim to investigate the quality of immunohistochemical
(IHC) ER-evaluation in FFPE-tissue over time (1978–2000).
Method: Tissue-microarrays from a Swedish multicenter cohort of 728
patients with contralateral BC was used for ER IHC-evaluation. BC was
studied in three periods (1958–1985, 1986–1993, 1994–2000), and retrospective
ER IHC-data was correlated to corresponding prospective ER
cytosol-analysis performed on fresh BC-tissue.
Results: The concordance between the original ER cytosol-analysis and
the new IHC was substantial (1978–1985: 82 %, (117/142), Kappa 0.52.
1986–1993: 91 %, (194/213), Kappa 0.72. 1994–2000: 86 %, (187/218),
Kappa 0.61). Discrepancies were mostly found for tumours with ERvalues
close to cutoff for one or both of the methods.
Conclusion: FFPE BC-tissue from the late 70s to millennium shows
preserved ER-antigenicity up to 35 years later. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Virchows Archiv
volume
471
issue
Suppl. 1
pages
1 pages
publisher
Springer
conference name
29th European Congress of Pathology
ISSN
1432-2307
DOI
10.1007/s00428-017-2205-0
language
English
LU publication?
yes
id
4a6e8d8d-9ae3-48d0-9803-666bbe3c203c
date added to LUP
2017-09-02 08:11:57
date last changed
2017-09-12 11:12:48
@misc{4a6e8d8d-9ae3-48d0-9803-666bbe3c203c,
  abstract     = {Objective: Estrogen receptor (ER) positive breast cancer (BC) can have<br/>an insidious course with disease-relapse decades after primary surgery.<br/>New analysis performed on archived formalin-fixed paraffin-embedded(FFPE) tissue are important for disease-management in late BC-relapse<br/>and an important tool in BC-research. However, although loss of immunoreactivity<br/>in tissue slides after sectioning has been shown, little is<br/>known of the preservation of biomarker-expression in FFPE tumourblocks.<br/>We aim to investigate the quality of immunohistochemical<br/>(IHC) ER-evaluation in FFPE-tissue over time (1978–2000).<br/>Method: Tissue-microarrays from a Swedish multicenter cohort of 728<br/>patients with contralateral BC was used for ER IHC-evaluation. BC was<br/>studied in three periods (1958–1985, 1986–1993, 1994–2000), and retrospective<br/>ER IHC-data was correlated to corresponding prospective ER<br/>cytosol-analysis performed on fresh BC-tissue.<br/>Results: The concordance between the original ER cytosol-analysis and<br/>the new IHC was substantial (1978–1985: 82 %, (117/142), Kappa 0.52.<br/>1986–1993: 91 %, (194/213), Kappa 0.72. 1994–2000: 86 %, (187/218),<br/>Kappa 0.61). Discrepancies were mostly found for tumours with ERvalues<br/>close to cutoff for one or both of the methods.<br/>Conclusion: FFPE BC-tissue from the late 70s to millennium shows<br/>preserved ER-antigenicity up to 35 years later.},
  articleno    = {PS-01-016},
  author       = {Ehinger, Anna and Rydén, Lisa and Bendahl, Pär-Ola and Fernö, Mårten and Alkner, Sara},
  issn         = {1432-2307},
  language     = {eng},
  month        = {09},
  note         = {Conference Abstract},
  number       = {Suppl. 1},
  pages        = {54--54},
  publisher    = {Springer},
  series       = {Virchows Archiv},
  title        = {Quality of up to 35 years old archival breast cancer tissue in paraffinblocks for estrogen receptor evaluation},
  url          = {http://dx.doi.org/10.1007/s00428-017-2205-0},
  volume       = {471},
  year         = {2017},
}