Molecular and cytological analysis of the expression of Streptomyces sporulation regulatory gene whiH.
(2013) In FEMS Microbiology Letters 341(2). p.96-105- Abstract
- The whiH gene is required for the orderly sporulation septation that divides aerial hyphae into spores in Streptomyces coelicolor. Here, we use a whiHp-mCherry transcriptional reporter construct to show that whiHp is active specifically in aerial hyphae, fluorescence being dependent on sporulation sigma factor WhiG. The results show that the promoter is active before the septation event that separates the sub-apical compartment from the tip compartment destined to become a spore chain. We conclude that WhiG-directed RNA polymerase activity, which is required for whiH transcription, must precede this septation event and is not restricted to apical sporogenic compartment of the aerial hyphae. Further, it is demonstrated that WhiH, a... (More)
- The whiH gene is required for the orderly sporulation septation that divides aerial hyphae into spores in Streptomyces coelicolor. Here, we use a whiHp-mCherry transcriptional reporter construct to show that whiHp is active specifically in aerial hyphae, fluorescence being dependent on sporulation sigma factor WhiG. The results show that the promoter is active before the septation event that separates the sub-apical compartment from the tip compartment destined to become a spore chain. We conclude that WhiG-directed RNA polymerase activity, which is required for whiH transcription, must precede this septation event and is not restricted to apical sporogenic compartment of the aerial hyphae. Further, it is demonstrated that WhiH, a predicted member of the GntR family of transcription factors, is able to bind specifically to a sequence in its own promoter, strongly suggesting that it acts as an autoregulatory transcription factor. Finally, we show by site-directed mutagenesis and a genetic complementation test that whiH is translated from a start codon overlapping with the previously identified transcription start point, implying leaderless transcription. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3559939
- author
- Persson, Jessica ; Chater, Keith F and Flärdh, Klas LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- GntR family, whiG, FtsZ, differentiation, developmental regulation, cell division
- in
- FEMS Microbiology Letters
- volume
- 341
- issue
- 2
- pages
- 96 - 105
- publisher
- Oxford University Press
- external identifiers
-
- wos:000316911800004
- pmid:23398592
- scopus:84875684178
- pmid:23398592
- ISSN
- 1574-6968
- DOI
- 10.1111/1574-6968.12099
- language
- English
- LU publication?
- yes
- id
- 4cbfe2d7-af52-4a29-94c8-6a5ee1430d36 (old id 3559939)
- date added to LUP
- 2016-04-01 10:00:27
- date last changed
- 2024-02-04 17:22:44
@article{4cbfe2d7-af52-4a29-94c8-6a5ee1430d36,
abstract = {{The whiH gene is required for the orderly sporulation septation that divides aerial hyphae into spores in Streptomyces coelicolor. Here, we use a whiHp-mCherry transcriptional reporter construct to show that whiHp is active specifically in aerial hyphae, fluorescence being dependent on sporulation sigma factor WhiG. The results show that the promoter is active before the septation event that separates the sub-apical compartment from the tip compartment destined to become a spore chain. We conclude that WhiG-directed RNA polymerase activity, which is required for whiH transcription, must precede this septation event and is not restricted to apical sporogenic compartment of the aerial hyphae. Further, it is demonstrated that WhiH, a predicted member of the GntR family of transcription factors, is able to bind specifically to a sequence in its own promoter, strongly suggesting that it acts as an autoregulatory transcription factor. Finally, we show by site-directed mutagenesis and a genetic complementation test that whiH is translated from a start codon overlapping with the previously identified transcription start point, implying leaderless transcription.}},
author = {{Persson, Jessica and Chater, Keith F and Flärdh, Klas}},
issn = {{1574-6968}},
keywords = {{GntR family; whiG; FtsZ; differentiation; developmental regulation; cell division}},
language = {{eng}},
number = {{2}},
pages = {{96--105}},
publisher = {{Oxford University Press}},
series = {{FEMS Microbiology Letters}},
title = {{Molecular and cytological analysis of the expression of Streptomyces sporulation regulatory gene whiH.}},
url = {{http://dx.doi.org/10.1111/1574-6968.12099}},
doi = {{10.1111/1574-6968.12099}},
volume = {{341}},
year = {{2013}},
}