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Early Postreperfusion Proteomics Reveal Divergent Inflammatory Responses in Kidney Transplantation with Implications on Outcomes

Strandberg, Gabriel LU ; Raihle, Carl LU ; Nilsson, Bo ; Öberg, Carl M LU ; Blom, Anna M LU orcid ; Axman, Sara ; Slivca, Oleg LU ; Paul, Clara LU ; Berglund, David and Biglarnia, Ali-Reza LU orcid (2025) In Transplantation p.1-9
Abstract

BACKGROUND: Ischemia/reperfusion injury is an unavoidable consequence of kidney transplantation, yet the characteristics of the immediate immune response after reperfusion and its impact on transplant outcomes remain poorly characterized in the clinical setting.

METHODS: We conducted a cohort study including 63 kidney transplant recipients (26 living-donor, 37 deceased-donor) with an extended 4-y follow-up to characterize early postreperfusion inflammatory dynamics and their association with transplant outcomes. Using high-throughput proteomics, we profiled 92 inflammatory markers in the early reperfusion stage. Intraoperative blood samples were collected systemically at baseline and from the transplant vein at 1, 10, and 30 min... (More)

BACKGROUND: Ischemia/reperfusion injury is an unavoidable consequence of kidney transplantation, yet the characteristics of the immediate immune response after reperfusion and its impact on transplant outcomes remain poorly characterized in the clinical setting.

METHODS: We conducted a cohort study including 63 kidney transplant recipients (26 living-donor, 37 deceased-donor) with an extended 4-y follow-up to characterize early postreperfusion inflammatory dynamics and their association with transplant outcomes. Using high-throughput proteomics, we profiled 92 inflammatory markers in the early reperfusion stage. Intraoperative blood samples were collected systemically at baseline and from the transplant vein at 1, 10, and 30 min postreperfusion.

RESULTS: Our analysis revealed a pronounced early immune response on reperfusion, with distinct inflammatory trajectories between living- and deceased-donor kidney allografts. Living-donor allografts showed proteomic patterns suggestive of a regulatory response, whereas deceased-donor allografts exhibited patterns associated with a cell injury-related response. Notably, interleukin-33 and hepatocyte growth factor were associated with delayed graft function, whereas hepatocyte growth factor also correlated with long-term allograft dysfunction.

CONCLUSIONS: These findings underscore the potential of assessing early postreperfusion inflammation to improve clinical risk stratification and guide future biomarker validation efforts.

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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
epub
subject
in
Transplantation
pages
1 - 9
publisher
Lippincott Williams & Wilkins
external identifiers
  • pmid:41171603
ISSN
1534-6080
DOI
10.1097/TP.0000000000005561
project
Kartläggning av inflammatoriska processer vid och efter organ transplantation - en stor longitudinell kohort studie
language
English
LU publication?
yes
additional info
Copyright © 2025 The Author(s). Published by Wolters Kluwer Health, Inc.
id
4cd0eec6-fe47-4aba-94d2-21fb1ab14fd7
date added to LUP
2025-12-17 14:08:54
date last changed
2025-12-19 02:25:40
@article{4cd0eec6-fe47-4aba-94d2-21fb1ab14fd7,
  abstract     = {{<p>BACKGROUND: Ischemia/reperfusion injury is an unavoidable consequence of kidney transplantation, yet the characteristics of the immediate immune response after reperfusion and its impact on transplant outcomes remain poorly characterized in the clinical setting.</p><p>METHODS: We conducted a cohort study including 63 kidney transplant recipients (26 living-donor, 37 deceased-donor) with an extended 4-y follow-up to characterize early postreperfusion inflammatory dynamics and their association with transplant outcomes. Using high-throughput proteomics, we profiled 92 inflammatory markers in the early reperfusion stage. Intraoperative blood samples were collected systemically at baseline and from the transplant vein at 1, 10, and 30 min postreperfusion.</p><p>RESULTS: Our analysis revealed a pronounced early immune response on reperfusion, with distinct inflammatory trajectories between living- and deceased-donor kidney allografts. Living-donor allografts showed proteomic patterns suggestive of a regulatory response, whereas deceased-donor allografts exhibited patterns associated with a cell injury-related response. Notably, interleukin-33 and hepatocyte growth factor were associated with delayed graft function, whereas hepatocyte growth factor also correlated with long-term allograft dysfunction.</p><p>CONCLUSIONS: These findings underscore the potential of assessing early postreperfusion inflammation to improve clinical risk stratification and guide future biomarker validation efforts.</p>}},
  author       = {{Strandberg, Gabriel and Raihle, Carl and Nilsson, Bo and Öberg, Carl M and Blom, Anna M and Axman, Sara and Slivca, Oleg and Paul, Clara and Berglund, David and Biglarnia, Ali-Reza}},
  issn         = {{1534-6080}},
  language     = {{eng}},
  month        = {{10}},
  pages        = {{1--9}},
  publisher    = {{Lippincott Williams & Wilkins}},
  series       = {{Transplantation}},
  title        = {{Early Postreperfusion Proteomics Reveal Divergent Inflammatory Responses in Kidney Transplantation with Implications on Outcomes}},
  url          = {{http://dx.doi.org/10.1097/TP.0000000000005561}},
  doi          = {{10.1097/TP.0000000000005561}},
  year         = {{2025}},
}