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The alarmins high mobility group box protein 1 and S100A8/A9 display different inflammatory profiles after acute knee injury

Aulin, C. LU ; Larsson, S. LU orcid ; Vogl, T. ; Roth, J. ; Åkesson, A. LU ; Swärd, P. LU ; Heinbäck, R. ; Erlandsson Harris, H. and Struglics, A. LU (2022) In Osteoarthritis and Cartilage 30(9). p.1198-1209
Abstract

Objective: To compare the concentrations of high mobility group box 1 protein (HMGB1) and S100A8/A9 in synovial fluid between patients with knee injuries and osteoarthritis (OA), and knee healthy subjects. To investigate associations of alarmin levels with different joint injuries and with biomarkers of inflammation, Wnt signaling, complement system, bone and cartilage degradation. Methods: HMGB1 and S100A8/A9 were measured in synovial fluid by immunoassays in patients with knee injuries, with OA and from knee healthy subjects, and were related to time from injury and with biomarkers obtained from previous studies. Hierarchical cluster and enrichment analyses of biomarkers associated to HMGB1 and S100A8/A9 were performed. Results: The... (More)

Objective: To compare the concentrations of high mobility group box 1 protein (HMGB1) and S100A8/A9 in synovial fluid between patients with knee injuries and osteoarthritis (OA), and knee healthy subjects. To investigate associations of alarmin levels with different joint injuries and with biomarkers of inflammation, Wnt signaling, complement system, bone and cartilage degradation. Methods: HMGB1 and S100A8/A9 were measured in synovial fluid by immunoassays in patients with knee injuries, with OA and from knee healthy subjects, and were related to time from injury and with biomarkers obtained from previous studies. Hierarchical cluster and enrichment analyses of biomarkers associated to HMGB1 and S100A8/A9 were performed. Results: The synovial fluid HMGB1 and S100A8/A9 concentrations were increased early after knee injury; S100A8/A9 levels were negatively associated to time after injury and was lower in the old compared to recent injury group, while HMGB1 was not associated to time after injury. The S100A8/A9 levels were also increased in OA. The initial inflammatory response was similar between the alarmins, and HMGB1 and S100A8/A9 shared 9 out of 20 enriched pathways. The alarmins displayed distinct response profiles, HMGB1 being associated to cartilage biomarkers while S100A8/A9 was associated to proinflammatory cytokines. Conclusions: HMGB1 and S100A8/A9 are increased as an immediate response to knee trauma. While they share many features in inflammatory and immunoregulatory mechanisms, S100A8/A9 and HMGB1 are associated to different downstream responses, which may have impact on the OA progression after acute knee injuries.

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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Alarmins, HMGB1, Knee injury, Osteoarthritis, S100A8/A9
in
Osteoarthritis and Cartilage
volume
30
issue
9
pages
12 pages
publisher
Elsevier
external identifiers
  • scopus:85135510355
  • pmid:35809846
ISSN
1063-4584
DOI
10.1016/j.joca.2022.06.009
language
English
LU publication?
yes
id
4ce6e754-9f90-45cc-84ac-a84b08ab84f8
date added to LUP
2022-10-07 12:52:20
date last changed
2024-06-13 20:00:07
@article{4ce6e754-9f90-45cc-84ac-a84b08ab84f8,
  abstract     = {{<p>Objective: To compare the concentrations of high mobility group box 1 protein (HMGB1) and S100A8/A9 in synovial fluid between patients with knee injuries and osteoarthritis (OA), and knee healthy subjects. To investigate associations of alarmin levels with different joint injuries and with biomarkers of inflammation, Wnt signaling, complement system, bone and cartilage degradation. Methods: HMGB1 and S100A8/A9 were measured in synovial fluid by immunoassays in patients with knee injuries, with OA and from knee healthy subjects, and were related to time from injury and with biomarkers obtained from previous studies. Hierarchical cluster and enrichment analyses of biomarkers associated to HMGB1 and S100A8/A9 were performed. Results: The synovial fluid HMGB1 and S100A8/A9 concentrations were increased early after knee injury; S100A8/A9 levels were negatively associated to time after injury and was lower in the old compared to recent injury group, while HMGB1 was not associated to time after injury. The S100A8/A9 levels were also increased in OA. The initial inflammatory response was similar between the alarmins, and HMGB1 and S100A8/A9 shared 9 out of 20 enriched pathways. The alarmins displayed distinct response profiles, HMGB1 being associated to cartilage biomarkers while S100A8/A9 was associated to proinflammatory cytokines. Conclusions: HMGB1 and S100A8/A9 are increased as an immediate response to knee trauma. While they share many features in inflammatory and immunoregulatory mechanisms, S100A8/A9 and HMGB1 are associated to different downstream responses, which may have impact on the OA progression after acute knee injuries.</p>}},
  author       = {{Aulin, C. and Larsson, S. and Vogl, T. and Roth, J. and Åkesson, A. and Swärd, P. and Heinbäck, R. and Erlandsson Harris, H. and Struglics, A.}},
  issn         = {{1063-4584}},
  keywords     = {{Alarmins; HMGB1; Knee injury; Osteoarthritis; S100A8/A9}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{1198--1209}},
  publisher    = {{Elsevier}},
  series       = {{Osteoarthritis and Cartilage}},
  title        = {{The alarmins high mobility group box protein 1 and S100A8/A9 display different inflammatory profiles after acute knee injury}},
  url          = {{http://dx.doi.org/10.1016/j.joca.2022.06.009}},
  doi          = {{10.1016/j.joca.2022.06.009}},
  volume       = {{30}},
  year         = {{2022}},
}