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Effects of drying methods on physical properties and morphology of trehalose/mannitol mixtures

Osanlóo, Daniel Tristan LU orcid ; Fransson, Jonas ; Bergenståhl, Björn LU and Millqvist-Fureby, Anna (2023) In Drying Technology 41(4). p.503-522
Abstract

Solid-state properties of dried protein formulations are important for stability and functionality of the product. This study investigates how different drying technologies (freeze-drying with and without annealing, spray drying and spray-freeze drying) affect the structure and solid-state properties of a set of matrix formulations composed of trehalose (glass former) and mannitol (scaffolding agent) in five ratios. The dried materials were characterized using differential scanning calorimetry, thermogravimetric analysis, x-ray diffraction and scanning electron microscopy. The morphology of the dried matrix is determined by the drying technology and the composition. In all mixtures, mannitol partially dissolved in the amorphous... (More)

Solid-state properties of dried protein formulations are important for stability and functionality of the product. This study investigates how different drying technologies (freeze-drying with and without annealing, spray drying and spray-freeze drying) affect the structure and solid-state properties of a set of matrix formulations composed of trehalose (glass former) and mannitol (scaffolding agent) in five ratios. The dried materials were characterized using differential scanning calorimetry, thermogravimetric analysis, x-ray diffraction and scanning electron microscopy. The morphology of the dried matrix is determined by the drying technology and the composition. In all mixtures, mannitol partially dissolved in the amorphous trehalose, resulting in reduced glass transition temperature. At least 50% mannitol is required to achieve a scaffolding effect through crystallized mannitol. At 25% mannitol poor structural stability is obtained regardless of drying technology. Despite the vast differences in drying kinetics, all drying technologies resulted in similar amorphous content in the dried material.

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Freeze-drying, morphology, scaffolding, solid-state, spray drying, spray-freeze drying
in
Drying Technology
volume
41
issue
4
pages
503 - 522
publisher
TAPPI
external identifiers
  • scopus:85135627393
ISSN
0737-3937
DOI
10.1080/07373937.2022.2103564
language
English
LU publication?
yes
id
4d47f0fe-d002-4bc4-9d90-3272909b93dc
date added to LUP
2022-09-19 10:19:38
date last changed
2023-12-20 01:29:54
@article{4d47f0fe-d002-4bc4-9d90-3272909b93dc,
  abstract     = {{<p>Solid-state properties of dried protein formulations are important for stability and functionality of the product. This study investigates how different drying technologies (freeze-drying with and without annealing, spray drying and spray-freeze drying) affect the structure and solid-state properties of a set of matrix formulations composed of trehalose (glass former) and mannitol (scaffolding agent) in five ratios. The dried materials were characterized using differential scanning calorimetry, thermogravimetric analysis, x-ray diffraction and scanning electron microscopy. The morphology of the dried matrix is determined by the drying technology and the composition. In all mixtures, mannitol partially dissolved in the amorphous trehalose, resulting in reduced glass transition temperature. At least 50% mannitol is required to achieve a scaffolding effect through crystallized mannitol. At 25% mannitol poor structural stability is obtained regardless of drying technology. Despite the vast differences in drying kinetics, all drying technologies resulted in similar amorphous content in the dried material.</p>}},
  author       = {{Osanlóo, Daniel Tristan and Fransson, Jonas and Bergenståhl, Björn and Millqvist-Fureby, Anna}},
  issn         = {{0737-3937}},
  keywords     = {{Freeze-drying; morphology; scaffolding; solid-state; spray drying; spray-freeze drying}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{503--522}},
  publisher    = {{TAPPI}},
  series       = {{Drying Technology}},
  title        = {{Effects of drying methods on physical properties and morphology of trehalose/mannitol mixtures}},
  url          = {{http://dx.doi.org/10.1080/07373937.2022.2103564}},
  doi          = {{10.1080/07373937.2022.2103564}},
  volume       = {{41}},
  year         = {{2023}},
}