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LGR5 Expression Predicting Poor Prognosis Is Negatively Correlated with WNT5A in Colon Cancer

Mehdawi, Lubna M. LU ; Ghatak, Souvik LU ; Chakraborty, Payel LU ; Sjölander, Anita LU and Andersson, Tommy LU (2023) In Cells 12(22).
Abstract

WNT/β-catenin signaling is essential for colon cancer development and progression. WNT5A (ligand of non-canonical WNT signaling) and its mimicking peptide Foxy5 impair β-catenin signaling in colon cancer cells via unknown mechanisms. Therefore, we investigated whether and how WNT5A signaling affects two promoters of β-catenin signaling: the LGR5 receptor and its ligand RSPO3, as well as β-catenin activity and its target gene VEGFA. Protein and gene expression in colon cancer cohorts were analyzed by immunohistochemistry and qRT-PCR, respectively. Three colon cancer cell lines were used for in vitro and one cell line for in vivo experiments and results were analyzed by Western blotting, RT-PCR, clonogenic and sphere formation assays,... (More)

WNT/β-catenin signaling is essential for colon cancer development and progression. WNT5A (ligand of non-canonical WNT signaling) and its mimicking peptide Foxy5 impair β-catenin signaling in colon cancer cells via unknown mechanisms. Therefore, we investigated whether and how WNT5A signaling affects two promoters of β-catenin signaling: the LGR5 receptor and its ligand RSPO3, as well as β-catenin activity and its target gene VEGFA. Protein and gene expression in colon cancer cohorts were analyzed by immunohistochemistry and qRT-PCR, respectively. Three colon cancer cell lines were used for in vitro and one cell line for in vivo experiments and results were analyzed by Western blotting, RT-PCR, clonogenic and sphere formation assays, immunofluorescence, and immunohistochemistry. Expression of WNT5A (a tumor suppressor) negatively correlated with that of LGR5/RSPO3 (tumor promoters) in colon cancer cohorts. Experimentally, WNT5A signaling suppressed β-catenin activity, LGR5, RSPO3, and VEGFA expression, and colony and spheroid formations. Since β-catenin signaling promotes colon cancer stemness, we explored how WNT5A expression is related to that of the cancer stem cell marker DCLK1. DCLK1 expression was negatively correlated with WNT5A expression in colon cancer cohorts and was experimentally reduced by WNT5A signaling. Thus, WNT5A and Foxy5 decrease LGR5/RSPO3 expression and β-catenin activity. This inhibits stemness and VEGFA expression, suggesting novel treatment strategies for the drug candidate Foxy5 in the handling of colon cancer patients.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Foxy5, LGR5, R-spondin3, VEGFA, WNT/β-catenin signaling, WNT5A
in
Cells
volume
12
issue
22
article number
2658
publisher
MDPI AG
external identifiers
  • pmid:37998393
  • scopus:85177848925
ISSN
2073-4409
DOI
10.3390/cells12222658
language
English
LU publication?
yes
id
4d6278d7-1a5e-4c46-9e4f-a1f659dd5399
date added to LUP
2024-01-09 10:39:31
date last changed
2024-04-24 06:36:35
@article{4d6278d7-1a5e-4c46-9e4f-a1f659dd5399,
  abstract     = {{<p>WNT/β-catenin signaling is essential for colon cancer development and progression. WNT5A (ligand of non-canonical WNT signaling) and its mimicking peptide Foxy5 impair β-catenin signaling in colon cancer cells via unknown mechanisms. Therefore, we investigated whether and how WNT5A signaling affects two promoters of β-catenin signaling: the LGR5 receptor and its ligand RSPO3, as well as β-catenin activity and its target gene VEGFA. Protein and gene expression in colon cancer cohorts were analyzed by immunohistochemistry and qRT-PCR, respectively. Three colon cancer cell lines were used for in vitro and one cell line for in vivo experiments and results were analyzed by Western blotting, RT-PCR, clonogenic and sphere formation assays, immunofluorescence, and immunohistochemistry. Expression of WNT5A (a tumor suppressor) negatively correlated with that of LGR5/RSPO3 (tumor promoters) in colon cancer cohorts. Experimentally, WNT5A signaling suppressed β-catenin activity, LGR5, RSPO3, and VEGFA expression, and colony and spheroid formations. Since β-catenin signaling promotes colon cancer stemness, we explored how WNT5A expression is related to that of the cancer stem cell marker DCLK1. DCLK1 expression was negatively correlated with WNT5A expression in colon cancer cohorts and was experimentally reduced by WNT5A signaling. Thus, WNT5A and Foxy5 decrease LGR5/RSPO3 expression and β-catenin activity. This inhibits stemness and VEGFA expression, suggesting novel treatment strategies for the drug candidate Foxy5 in the handling of colon cancer patients.</p>}},
  author       = {{Mehdawi, Lubna M. and Ghatak, Souvik and Chakraborty, Payel and Sjölander, Anita and Andersson, Tommy}},
  issn         = {{2073-4409}},
  keywords     = {{Foxy5; LGR5; R-spondin3; VEGFA; WNT/β-catenin signaling; WNT5A}},
  language     = {{eng}},
  number       = {{22}},
  publisher    = {{MDPI AG}},
  series       = {{Cells}},
  title        = {{LGR5 Expression Predicting Poor Prognosis Is Negatively Correlated with WNT5A in Colon Cancer}},
  url          = {{http://dx.doi.org/10.3390/cells12222658}},
  doi          = {{10.3390/cells12222658}},
  volume       = {{12}},
  year         = {{2023}},
}