MANF is widely expressed in mammalian tissues and differently regulated after ischemic and epileptic insults in rodent brain
(2008) In Molecular and Cellular Neuroscience 39(3). p.356-371- Abstract
- The mesencephalic astrocyte-derived neurotrophic factor (MANF) has been described as a Survival factor for dopaminergic neurons in vitro, but its expression in mammalian tissues is poorly known. MANF and a homologous Protein, the conserved dopamine neurotrophic factor (CDNF), form a novel evolutionary conserved family of neurotrophic factors. Here we used in situ hybridization and immunohistochemistry to characterize MANF expression in developing and adult mouse. MANF expression was widespread in the nervous system and non-neuronal tissues. In the brain, relatively high MANF levels were detected in the cerebral cortex, hippocampus and cerebellar Purkinje cells. After status epilepticus, Manf mRNA expression was transiently increased in the... (More)
- The mesencephalic astrocyte-derived neurotrophic factor (MANF) has been described as a Survival factor for dopaminergic neurons in vitro, but its expression in mammalian tissues is poorly known. MANF and a homologous Protein, the conserved dopamine neurotrophic factor (CDNF), form a novel evolutionary conserved family of neurotrophic factors. Here we used in situ hybridization and immunohistochemistry to characterize MANF expression in developing and adult mouse. MANF expression was widespread in the nervous system and non-neuronal tissues. In the brain, relatively high MANF levels were detected in the cerebral cortex, hippocampus and cerebellar Purkinje cells. After status epilepticus, Manf mRNA expression was transiently increased in the dentate granule cell layer of hippocampus, thalamic reticular nucleus and in several cortical areas. In contrast, following global forebrain ischemia changes in Manf expression were widespread in the hippocampal formation and more restricted in cerebral cortex. The widespread expression of MANF together with its evolutionary conserved nature and regulation by brain insults suggest that it has important functions both under normal and pathological conditions in many tissue types. (C) 2008 Elsevier Inc. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1282977
- author
- Lindholm, Paivi ; Peranen, Johan ; Andressoo, Jaan-Olle ; Kalkkinen, Nisse ; Kokaia, Zaal LU ; Lindvall, Olle LU ; Timmusk, Tonis and Saarma, Mart
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Status epilepticus, Ischemia, Immunohistochemistry, Expression, Dopaminergic, CDNF, MANF, ARMET
- in
- Molecular and Cellular Neuroscience
- volume
- 39
- issue
- 3
- pages
- 356 - 371
- publisher
- Elsevier
- external identifiers
-
- wos:000260607600007
- scopus:53849102168
- ISSN
- 1044-7431
- DOI
- 10.1016/j.mcn.2008.07.016
- language
- English
- LU publication?
- yes
- id
- 4d76ea45-4392-45ca-957c-aa787fed46fa (old id 1282977)
- date added to LUP
- 2016-04-01 12:08:21
- date last changed
- 2022-04-13 06:32:44
@article{4d76ea45-4392-45ca-957c-aa787fed46fa, abstract = {{The mesencephalic astrocyte-derived neurotrophic factor (MANF) has been described as a Survival factor for dopaminergic neurons in vitro, but its expression in mammalian tissues is poorly known. MANF and a homologous Protein, the conserved dopamine neurotrophic factor (CDNF), form a novel evolutionary conserved family of neurotrophic factors. Here we used in situ hybridization and immunohistochemistry to characterize MANF expression in developing and adult mouse. MANF expression was widespread in the nervous system and non-neuronal tissues. In the brain, relatively high MANF levels were detected in the cerebral cortex, hippocampus and cerebellar Purkinje cells. After status epilepticus, Manf mRNA expression was transiently increased in the dentate granule cell layer of hippocampus, thalamic reticular nucleus and in several cortical areas. In contrast, following global forebrain ischemia changes in Manf expression were widespread in the hippocampal formation and more restricted in cerebral cortex. The widespread expression of MANF together with its evolutionary conserved nature and regulation by brain insults suggest that it has important functions both under normal and pathological conditions in many tissue types. (C) 2008 Elsevier Inc. All rights reserved.}}, author = {{Lindholm, Paivi and Peranen, Johan and Andressoo, Jaan-Olle and Kalkkinen, Nisse and Kokaia, Zaal and Lindvall, Olle and Timmusk, Tonis and Saarma, Mart}}, issn = {{1044-7431}}, keywords = {{Status epilepticus; Ischemia; Immunohistochemistry; Expression; Dopaminergic; CDNF; MANF; ARMET}}, language = {{eng}}, number = {{3}}, pages = {{356--371}}, publisher = {{Elsevier}}, series = {{Molecular and Cellular Neuroscience}}, title = {{MANF is widely expressed in mammalian tissues and differently regulated after ischemic and epileptic insults in rodent brain}}, url = {{http://dx.doi.org/10.1016/j.mcn.2008.07.016}}, doi = {{10.1016/j.mcn.2008.07.016}}, volume = {{39}}, year = {{2008}}, }