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B-lineage commitment prior to surface expression of B220 and CD19 on hematopoietic progenitor cells

Månsson, Robert LU ; Zandi, Sasan ; Anderson, Kristina LU ; Martensson, Inga-Lill ; Jacobsen, Sten Eirik W LU ; Bryder, David LU and Sigvardsson, Mikael LU (2008) In Blood 112(4). p.1048-1055
Abstract
Commitment of hematopoietic progenitor cells to B-lymphoid cell fate has been suggested to coincide with the development of PAX5-expressing B220(+)CD19(+) pro-B cells. We have used a transgenic reporter mouse, expressing human CD25 under the control of the B-lineage-restricted Igll1 (lambda 5) promoter to investigate the lineage potential of early progenitor cells in the bone marrow. This strategy allowed us to identify a reporter expressing LIN-B220(-)CD19(-)CD127(+)FLT3(+) SCA1(low)KIT(low) population that displays a lack of myeloid and a 90% reduction in in vitro T-cell potential compared with its reporter-negative counterpart. Gene expression analysis demonstrated that these lineage-restricted cells express B-lineage-associated genes... (More)
Commitment of hematopoietic progenitor cells to B-lymphoid cell fate has been suggested to coincide with the development of PAX5-expressing B220(+)CD19(+) pro-B cells. We have used a transgenic reporter mouse, expressing human CD25 under the control of the B-lineage-restricted Igll1 (lambda 5) promoter to investigate the lineage potential of early progenitor cells in the bone marrow. This strategy allowed us to identify a reporter expressing LIN-B220(-)CD19(-)CD127(+)FLT3(+) SCA1(low)KIT(low) population that displays a lack of myeloid and a 90% reduction in in vitro T-cell potential compared with its reporter-negative counterpart. Gene expression analysis demonstrated that these lineage-restricted cells express B-lineage-associated genes to levels comparable with that observed in pro-B cells. These data suggest that B-lineage commitment can occur before the expression of B220 and CD19. (Less)
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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Blood
volume
112
issue
4
pages
1048 - 1055
publisher
American Society of Hematology
external identifiers
  • wos:000258392300026
  • scopus:50849143861
  • pmid:18495958
ISSN
1528-0020
DOI
10.1182/blood-2007-11-125385
language
English
LU publication?
yes
id
4f074ea8-aec4-4c1f-9e78-33f2716ed684 (old id 1252912)
date added to LUP
2016-04-01 12:24:21
date last changed
2022-07-22 20:08:14
@article{4f074ea8-aec4-4c1f-9e78-33f2716ed684,
  abstract     = {{Commitment of hematopoietic progenitor cells to B-lymphoid cell fate has been suggested to coincide with the development of PAX5-expressing B220(+)CD19(+) pro-B cells. We have used a transgenic reporter mouse, expressing human CD25 under the control of the B-lineage-restricted Igll1 (lambda 5) promoter to investigate the lineage potential of early progenitor cells in the bone marrow. This strategy allowed us to identify a reporter expressing LIN-B220(-)CD19(-)CD127(+)FLT3(+) SCA1(low)KIT(low) population that displays a lack of myeloid and a 90% reduction in in vitro T-cell potential compared with its reporter-negative counterpart. Gene expression analysis demonstrated that these lineage-restricted cells express B-lineage-associated genes to levels comparable with that observed in pro-B cells. These data suggest that B-lineage commitment can occur before the expression of B220 and CD19.}},
  author       = {{Månsson, Robert and Zandi, Sasan and Anderson, Kristina and Martensson, Inga-Lill and Jacobsen, Sten Eirik W and Bryder, David and Sigvardsson, Mikael}},
  issn         = {{1528-0020}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{1048--1055}},
  publisher    = {{American Society of Hematology}},
  series       = {{Blood}},
  title        = {{B-lineage commitment prior to surface expression of B220 and CD19 on hematopoietic progenitor cells}},
  url          = {{http://dx.doi.org/10.1182/blood-2007-11-125385}},
  doi          = {{10.1182/blood-2007-11-125385}},
  volume       = {{112}},
  year         = {{2008}},
}