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Community dynamics of arbuscular mycorrhizal fungi in high-input and intensively irrigated rice cultivation systems.

Wang, Yutao; Li, Ting; Li, Yingwei; Björn, Lars Olof LU ; Rosendahl, Søren; Olsson, Pål Axel LU ; Li, Shaoshan and Fu, Xuelin (2015) In Applied and Environmental Microbiology 81(8). p.2958-2965
Abstract
Application of mycorrhizal inoculum could be one way to increase the yield of rice plants, and reduce the application of fertilizer. We therefore studied arbuscular mycorrhizal fungi (AMF) in the roots of wetland rice (Oryza sativa L.) collected at the seedling, tillering, heading and ripening stages in four paddy wetlands that had been under a high-input and intensively irrigated rice cultivation system for more than 20 years. It was found that AMF colonization was mainly established in the heading and ripening stages. The AMF community structure was characterized in rhizosphere soil and root from two of the studied paddy wetlands. A fragment covering partial SSU, the whole ITS and partial LSU rRNA operon region of AMF was amplified,... (More)
Application of mycorrhizal inoculum could be one way to increase the yield of rice plants, and reduce the application of fertilizer. We therefore studied arbuscular mycorrhizal fungi (AMF) in the roots of wetland rice (Oryza sativa L.) collected at the seedling, tillering, heading and ripening stages in four paddy wetlands that had been under a high-input and intensively irrigated rice cultivation system for more than 20 years. It was found that AMF colonization was mainly established in the heading and ripening stages. The AMF community structure was characterized in rhizosphere soil and root from two of the studied paddy wetlands. A fragment covering partial SSU, the whole ITS and partial LSU rRNA operon region of AMF was amplified, cloned and sequenced from roots and soils. A total of 639 AMF sequences were obtained, and these were finally assigned to 16 phylotypes based on a phylogenetic analysis, including 12 phylotypes from Glomeraceae, one phylotype from Claroideoglomeraceae, two phylotypes from Paraglomeraceae and one unidentified phylotype. The AMF phylotype compositions in the soils were similar between the two surveyed sites, but there was a clear discrepancy between the communities obtained from root and soil. The relatively high number of AMF phylotypes at the surveyed sites suggests that the conditions are suitable for some species of AMF and that they may have an important function in conventional rice cultivation systems. The species richness of root-colonizing AMF increased with growth of rice, and future studies should consider the developmental stages of this crop in the exploration of AMF function in paddy wetlands. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
community dynamic, arbuscular mycorrhizal fungi (AMF), diversity, growth stage, paddy wetland, rice (Oryza sativa)
in
Applied and Environmental Microbiology
volume
81
issue
8
pages
2958 - 2965
publisher
American Society for Microbiology
external identifiers
  • pmid:25681190
  • wos:000351843900032
  • scopus:84927798077
ISSN
0099-2240
DOI
10.1128/AEM.03769-14
project
Photobiology
language
English
LU publication?
yes
id
a7591a53-5cc0-4414-8b5e-06fd1fd757b9 (old id 5143723)
date added to LUP
2015-03-09 13:13:15
date last changed
2017-02-26 03:02:23
@article{a7591a53-5cc0-4414-8b5e-06fd1fd757b9,
  abstract     = {Application of mycorrhizal inoculum could be one way to increase the yield of rice plants, and reduce the application of fertilizer. We therefore studied arbuscular mycorrhizal fungi (AMF) in the roots of wetland rice (Oryza sativa L.) collected at the seedling, tillering, heading and ripening stages in four paddy wetlands that had been under a high-input and intensively irrigated rice cultivation system for more than 20 years. It was found that AMF colonization was mainly established in the heading and ripening stages. The AMF community structure was characterized in rhizosphere soil and root from two of the studied paddy wetlands. A fragment covering partial SSU, the whole ITS and partial LSU rRNA operon region of AMF was amplified, cloned and sequenced from roots and soils. A total of 639 AMF sequences were obtained, and these were finally assigned to 16 phylotypes based on a phylogenetic analysis, including 12 phylotypes from Glomeraceae, one phylotype from Claroideoglomeraceae, two phylotypes from Paraglomeraceae and one unidentified phylotype. The AMF phylotype compositions in the soils were similar between the two surveyed sites, but there was a clear discrepancy between the communities obtained from root and soil. The relatively high number of AMF phylotypes at the surveyed sites suggests that the conditions are suitable for some species of AMF and that they may have an important function in conventional rice cultivation systems. The species richness of root-colonizing AMF increased with growth of rice, and future studies should consider the developmental stages of this crop in the exploration of AMF function in paddy wetlands.},
  author       = {Wang, Yutao and Li, Ting and Li, Yingwei and Björn, Lars Olof and Rosendahl, Søren and Olsson, Pål Axel and Li, Shaoshan and Fu, Xuelin},
  issn         = {0099-2240},
  keyword      = {community dynamic,arbuscular mycorrhizal fungi (AMF),diversity,growth stage,paddy wetland,rice (Oryza sativa)},
  language     = {eng},
  number       = {8},
  pages        = {2958--2965},
  publisher    = {American Society for Microbiology},
  series       = {Applied and Environmental Microbiology},
  title        = {Community dynamics of arbuscular mycorrhizal fungi in high-input and intensively irrigated rice cultivation systems.},
  url          = {http://dx.doi.org/10.1128/AEM.03769-14},
  volume       = {81},
  year         = {2015},
}