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Induction of morphological changes in death-induced cancer cells monitored by holographic microscopy.

El-Schish, Zahra LU ; Mölder, Anna; Tassidis, Helena LU ; Härkönen, Pirkko LU ; Falck Miniotis, Maria LU and Gjörloff Wingren, Anette LU (2015) In Journal of Structural Biology 189(3). p.207-212
Abstract
We are using the label-free technique of holographic microscopy to analyze cellular parameters including cell number, confluence, cellular volume and area directly in the cell culture environment. We show that death-induced cells can be distinguished from untreated counterparts by the use of holographic microscopy, and we demonstrate its capability for cell death assessment. Morphological analysis of two representative cell lines (L929 and DU145) was performed in the culture flasks without any prior cell detachment. The two cell lines were treated with the anti-tumour agent etoposide for 1-3days. Measurements by holographic microscopy showed significant differences in average cell number, confluence, volume and area when comparing... (More)
We are using the label-free technique of holographic microscopy to analyze cellular parameters including cell number, confluence, cellular volume and area directly in the cell culture environment. We show that death-induced cells can be distinguished from untreated counterparts by the use of holographic microscopy, and we demonstrate its capability for cell death assessment. Morphological analysis of two representative cell lines (L929 and DU145) was performed in the culture flasks without any prior cell detachment. The two cell lines were treated with the anti-tumour agent etoposide for 1-3days. Measurements by holographic microscopy showed significant differences in average cell number, confluence, volume and area when comparing etoposide-treated with untreated cells. The cell volume of the treated cell lines was initially increased at early time-points. By time, cells decreased in volume, especially when treated with high doses of etoposide. In conclusion, we have shown that holographic microscopy allows label-free and completely non-invasive morphological measurements of cell growth, viability and death. Future applications could include real-time monitoring of these holographic microscopy parameters in cells in response to clinically relevant compounds. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Structural Biology
volume
189
issue
3
pages
207 - 212
publisher
Elsevier
external identifiers
  • pmid:25637284
  • wos:000351249500005
  • scopus:84923275881
ISSN
1095-8657
DOI
10.1016/j.jsb.2015.01.010
language
English
LU publication?
yes
id
403a0305-fe3f-495a-86f7-cd1b608f513d (old id 5145873)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25637284?dopt=Abstract
date added to LUP
2015-03-03 14:07:49
date last changed
2017-10-01 03:24:39
@article{403a0305-fe3f-495a-86f7-cd1b608f513d,
  abstract     = {We are using the label-free technique of holographic microscopy to analyze cellular parameters including cell number, confluence, cellular volume and area directly in the cell culture environment. We show that death-induced cells can be distinguished from untreated counterparts by the use of holographic microscopy, and we demonstrate its capability for cell death assessment. Morphological analysis of two representative cell lines (L929 and DU145) was performed in the culture flasks without any prior cell detachment. The two cell lines were treated with the anti-tumour agent etoposide for 1-3days. Measurements by holographic microscopy showed significant differences in average cell number, confluence, volume and area when comparing etoposide-treated with untreated cells. The cell volume of the treated cell lines was initially increased at early time-points. By time, cells decreased in volume, especially when treated with high doses of etoposide. In conclusion, we have shown that holographic microscopy allows label-free and completely non-invasive morphological measurements of cell growth, viability and death. Future applications could include real-time monitoring of these holographic microscopy parameters in cells in response to clinically relevant compounds.},
  author       = {El-Schish, Zahra and Mölder, Anna and Tassidis, Helena and Härkönen, Pirkko and Falck Miniotis, Maria and Gjörloff Wingren, Anette},
  issn         = {1095-8657},
  language     = {eng},
  number       = {3},
  pages        = {207--212},
  publisher    = {Elsevier},
  series       = {Journal of Structural Biology},
  title        = {Induction of morphological changes in death-induced cancer cells monitored by holographic microscopy.},
  url          = {http://dx.doi.org/10.1016/j.jsb.2015.01.010},
  volume       = {189},
  year         = {2015},
}