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The protective and therapeutic effect of exclusive and combined treatment with alpha-ketoglutarate sodium salt and ipriflavone on bone loss in orchidectomized rats

Radzki, R. P.; Bieńko, M.; Filip, R. and Pierzynowski, S. G. LU (2016) In Journal of Nutrition, Health and Aging 20(6). p.628-636
Abstract

Objective: This study investigated the effect of alpha-ketoglutarate sodium salt (AKG) and ipriflavone (IP) treatment on the mineralization of the tibia in male rats during the development and after the establishment of osteopenia. Design: One hundred and twenty eight male rats were randomly selected and submitted to either sham-operation (SHO) or orchidectomy (ORX), after which each group were then randomly divided between the two experiments. In Experiment-1, treatment with AKG or/and IP started after a 7-day recovery period, whereas in Experiment-2, the experimental protocol proceeded after a 60-day period of osteopenia establishment. AKG was then administered as an experimental drinking, at a concentration of 1.0 mol/l. As a... (More)

Objective: This study investigated the effect of alpha-ketoglutarate sodium salt (AKG) and ipriflavone (IP) treatment on the mineralization of the tibia in male rats during the development and after the establishment of osteopenia. Design: One hundred and twenty eight male rats were randomly selected and submitted to either sham-operation (SHO) or orchidectomy (ORX), after which each group were then randomly divided between the two experiments. In Experiment-1, treatment with AKG or/and IP started after a 7-day recovery period, whereas in Experiment-2, the experimental protocol proceeded after a 60-day period of osteopenia establishment. AKG was then administered as an experimental drinking, at a concentration of 1.0 mol/l. As a control, a placebo solution was administered. IP at 50 mg/kg b.w., and physiological saline–PhS (as a control for IP) were applied daily via gavage. Measurements: After 60 days of experimental treatment, in both experiments, the rats were sacrificed, their body weight recorded, while blood serum (Osteocalcin, CTX) and isolated tibia (weight, length, pQCT, DXA, 3-point bending test) were stored for further analysis. Results and conclusions: Our results show that during the development of osteopenia, AKG and IP when applied exclusively, counteracts osteopenia development, whereas their usage after the establishment of osteopenia, significantly limits the development of bone disorders. Furthermore, combined treatment of AKG and IP exceeded the effects of their sole usage. In addition, during the development of osteopenia, AKG and IP not only inhibited bone resorption, but markedly stimulated the formation of bone tissue. Finally, after the development of osteopenia, combined treatment with AKG and IP protected the bone tissue against orchidectomy-induced bone loss.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
alpha-ketoglutarate, densitometry, ipriflavone, Male osteoporosis, orchidectomy
in
Journal of Nutrition, Health and Aging
volume
20
issue
6
pages
9 pages
publisher
Springer-Verlag France
external identifiers
  • scopus:84951991906
  • wos:000378907300006
ISSN
1279-7707
DOI
10.1007/s12603-015-0654-1
language
English
LU publication?
yes
id
517b0549-0645-4f43-ab2b-88dd62c6de1d
date added to LUP
2017-01-27 08:13:11
date last changed
2017-09-18 11:34:26
@article{517b0549-0645-4f43-ab2b-88dd62c6de1d,
  abstract     = {<p>Objective: This study investigated the effect of alpha-ketoglutarate sodium salt (AKG) and ipriflavone (IP) treatment on the mineralization of the tibia in male rats during the development and after the establishment of osteopenia. Design: One hundred and twenty eight male rats were randomly selected and submitted to either sham-operation (SHO) or orchidectomy (ORX), after which each group were then randomly divided between the two experiments. In Experiment-1, treatment with AKG or/and IP started after a 7-day recovery period, whereas in Experiment-2, the experimental protocol proceeded after a 60-day period of osteopenia establishment. AKG was then administered as an experimental drinking, at a concentration of 1.0 mol/l. As a control, a placebo solution was administered. IP at 50 mg/kg b.w., and physiological saline–PhS (as a control for IP) were applied daily via gavage. Measurements: After 60 days of experimental treatment, in both experiments, the rats were sacrificed, their body weight recorded, while blood serum (Osteocalcin, CTX) and isolated tibia (weight, length, pQCT, DXA, 3-point bending test) were stored for further analysis. Results and conclusions: Our results show that during the development of osteopenia, AKG and IP when applied exclusively, counteracts osteopenia development, whereas their usage after the establishment of osteopenia, significantly limits the development of bone disorders. Furthermore, combined treatment of AKG and IP exceeded the effects of their sole usage. In addition, during the development of osteopenia, AKG and IP not only inhibited bone resorption, but markedly stimulated the formation of bone tissue. Finally, after the development of osteopenia, combined treatment with AKG and IP protected the bone tissue against orchidectomy-induced bone loss.</p>},
  author       = {Radzki, R. P. and Bieńko, M. and Filip, R. and Pierzynowski, S. G.},
  issn         = {1279-7707},
  keyword      = {alpha-ketoglutarate,densitometry,ipriflavone,Male osteoporosis,orchidectomy},
  language     = {eng},
  month        = {06},
  number       = {6},
  pages        = {628--636},
  publisher    = {Springer-Verlag France},
  series       = {Journal of Nutrition, Health and Aging},
  title        = {The protective and therapeutic effect of exclusive and combined treatment with alpha-ketoglutarate sodium salt and ipriflavone on bone loss in orchidectomized rats},
  url          = {http://dx.doi.org/10.1007/s12603-015-0654-1},
  volume       = {20},
  year         = {2016},
}