Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies)
(2004) 864. p.66-78- Abstract
- Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190degrees C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard... (More)
- Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190degrees C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard molecules were prepared using SEC. The structure of the AcGGM was determined using H-1-NMR. The enzymatic hydrolysis of AcGGM was studied using beta-mannanase and alpha-galactosidase (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/526694
- author
- organization
- publishing date
- 2004
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- MICROWAVE IRRADIATION, PINUS-SILVESTRIS L, DEGRADING ENZYMES, GLUCOMANNAN, LIGNOCELLULOSE, CHROMATOGRAPHY, PRETREATMENT, HEMICELLULOSES, BETA-MANNANASE, POSITIONS
- host publication
- Hemicelluloses. science and technology. (ACS symposium series ; 864 )
- volume
- 864
- pages
- 66 - 78
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- scopus:38849099768
- ISBN
- 0-8412-3842-1
- language
- English
- LU publication?
- yes
- id
- 8325438f-3cdc-4bb6-bfdb-7bafdd04a68c (old id 526694)
- date added to LUP
- 2016-04-04 10:31:45
- date last changed
- 2023-09-20 07:01:49
@inproceedings{8325438f-3cdc-4bb6-bfdb-7bafdd04a68c, abstract = {{Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190degrees C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard molecules were prepared using SEC. The structure of the AcGGM was determined using H-1-NMR. The enzymatic hydrolysis of AcGGM was studied using beta-mannanase and alpha-galactosidase}}, author = {{Stålbrand, Henrik and Lundqvist, Jon and Andersson, Alexandra and Hägglund, Per and Anderson, Lars and Tjerneld, Folke and Jacobs A,, A and Teleman, A and Dahlman, O and Palm, M and Zacchi, Guido}}, booktitle = {{Hemicelluloses. science and technology. (ACS symposium series ; 864 )}}, isbn = {{0-8412-3842-1}}, keywords = {{MICROWAVE IRRADIATION; PINUS-SILVESTRIS L; DEGRADING ENZYMES; GLUCOMANNAN; LIGNOCELLULOSE; CHROMATOGRAPHY; PRETREATMENT; HEMICELLULOSES; BETA-MANNANASE; POSITIONS}}, language = {{eng}}, pages = {{66--78}}, publisher = {{The American Chemical Society (ACS)}}, title = {{Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies)}}, volume = {{864}}, year = {{2004}}, }