Advanced

A novel method for downstream characterization of breast cancer circulating tumor cells following CellSearch isolation.

Frithiof, Henrik LU ; Welinder, Charlotte LU ; Larsson, Anna-Maria LU ; Rydén, Lisa LU and Aaltonen, Kristina LU (2015) In Journal of Translational Medicine 13(1).
Abstract
Enumeration of circulating tumor cells (CTCs) obtained from minimally invasive blood samples has been well established as a valuable monitoring tool in metastatic and early breast cancer, as well as in several other cancer types. The gold standard technology for detecting CTCs in blood against a backdrop of millions of leukocytes is the FDA-approved CellSearch system (Janssen Diagnostics), which relies on EpCAM-based immunomagnetic separation. Secondary characterization of these cells could enable treatment selection based on specific targets in these cells, as well as providing a real time window into the metastatic process and offering unique insights into tumor heterogeneity. The objective of this study was to develop a method for... (More)
Enumeration of circulating tumor cells (CTCs) obtained from minimally invasive blood samples has been well established as a valuable monitoring tool in metastatic and early breast cancer, as well as in several other cancer types. The gold standard technology for detecting CTCs in blood against a backdrop of millions of leukocytes is the FDA-approved CellSearch system (Janssen Diagnostics), which relies on EpCAM-based immunomagnetic separation. Secondary characterization of these cells could enable treatment selection based on specific targets in these cells, as well as providing a real time window into the metastatic process and offering unique insights into tumor heterogeneity. The objective of this study was to develop a method for downstream characterization of CTCs following isolation with the CellSearch system. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Translational Medicine
volume
13
issue
1
publisher
BioMed Central
external identifiers
  • pmid:25896421
  • scopus:84928529608
  • wos:000369792500001
ISSN
1479-5876
DOI
10.1186/s12967-015-0493-1
language
English
LU publication?
yes
id
8651c485-e00b-4260-841c-a25cc6ab14f3 (old id 5341114)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25896421?dopt=Abstract
date added to LUP
2015-05-04 10:52:14
date last changed
2017-07-23 04:14:44
@article{8651c485-e00b-4260-841c-a25cc6ab14f3,
  abstract     = {Enumeration of circulating tumor cells (CTCs) obtained from minimally invasive blood samples has been well established as a valuable monitoring tool in metastatic and early breast cancer, as well as in several other cancer types. The gold standard technology for detecting CTCs in blood against a backdrop of millions of leukocytes is the FDA-approved CellSearch system (Janssen Diagnostics), which relies on EpCAM-based immunomagnetic separation. Secondary characterization of these cells could enable treatment selection based on specific targets in these cells, as well as providing a real time window into the metastatic process and offering unique insights into tumor heterogeneity. The objective of this study was to develop a method for downstream characterization of CTCs following isolation with the CellSearch system.},
  articleno    = {126},
  author       = {Frithiof, Henrik and Welinder, Charlotte and Larsson, Anna-Maria and Rydén, Lisa and Aaltonen, Kristina},
  issn         = {1479-5876},
  language     = {eng},
  number       = {1},
  publisher    = {BioMed Central},
  series       = {Journal of Translational Medicine},
  title        = {A novel method for downstream characterization of breast cancer circulating tumor cells following CellSearch isolation.},
  url          = {http://dx.doi.org/10.1186/s12967-015-0493-1},
  volume       = {13},
  year         = {2015},
}