Advanced

Critical role of P-selectin-dependent leukocyte recruitment in endotoxin-induced intestinal barrier dysfunction in mice.

Mangell, Peter LU ; Röme, Andrada LU ; Wang, Yusheng LU ; Schramm, R; Jeppsson, Bengt LU and Thorlacius, Henrik LU (2007) In Inflammation Research 56(5). p.189-194
Abstract
Objective: To define the importance of leukocyte recruitment in endotoxin-induced gut permeability. Materials and methods: 31 male C57BL/6 mice were challenged with lipopolysaccharide (LPS). Ileal permeability was measured in Ussing chambers and leukocyte-endothelium interactions studied with intravital fluorescence microscopy after 18 h. Results: LPS caused a clear-cut increase in leukocyte accumulation and intestinal permeability. Immunoneutralisation of P-selectin not only reduced leukocyte recruitment significantly (54% reduction) but also abolished endotoxin-induced intestinal leakage. Intestinal levels of pro-inflammatory chemokines increased markedly in response to LPS but were not influenced by inhibition of P-selectin in vivo.... (More)
Objective: To define the importance of leukocyte recruitment in endotoxin-induced gut permeability. Materials and methods: 31 male C57BL/6 mice were challenged with lipopolysaccharide (LPS). Ileal permeability was measured in Ussing chambers and leukocyte-endothelium interactions studied with intravital fluorescence microscopy after 18 h. Results: LPS caused a clear-cut increase in leukocyte accumulation and intestinal permeability. Immunoneutralisation of P-selectin not only reduced leukocyte recruitment significantly (54% reduction) but also abolished endotoxin-induced intestinal leakage. Intestinal levels of pro-inflammatory chemokines increased markedly in response to LPS but were not influenced by inhibition of P-selectin in vivo. Conclusion: The present study shows not only that endotoxin-induced leukocyte recruitment is mediated by P-selectin but also that sepsis-associated intestinal leakage in the gut is largely regulated by leukocyte accumulation. Thus, our novel data demonstrate a critical link between P-selectin-dependent leukocyte recruitment and gut barrier failure in endotoxemia. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
P-selectin, leukocyte, sepsis, intestinal barrier, endotoxin
in
Inflammation Research
volume
56
issue
5
pages
189 - 194
publisher
Birkhaüser
external identifiers
  • wos:000246804400003
  • scopus:34250890739
ISSN
1420-908X
DOI
10.1007/s00011-007-6163-x
language
English
LU publication?
yes
id
175813ce-128b-4ae9-baa9-1d2595b0d121 (old id 539596)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17588134&dopt=Abstract
date added to LUP
2007-12-18 10:19:14
date last changed
2017-02-26 03:28:13
@article{175813ce-128b-4ae9-baa9-1d2595b0d121,
  abstract     = {Objective: To define the importance of leukocyte recruitment in endotoxin-induced gut permeability. Materials and methods: 31 male C57BL/6 mice were challenged with lipopolysaccharide (LPS). Ileal permeability was measured in Ussing chambers and leukocyte-endothelium interactions studied with intravital fluorescence microscopy after 18 h. Results: LPS caused a clear-cut increase in leukocyte accumulation and intestinal permeability. Immunoneutralisation of P-selectin not only reduced leukocyte recruitment significantly (54% reduction) but also abolished endotoxin-induced intestinal leakage. Intestinal levels of pro-inflammatory chemokines increased markedly in response to LPS but were not influenced by inhibition of P-selectin in vivo. Conclusion: The present study shows not only that endotoxin-induced leukocyte recruitment is mediated by P-selectin but also that sepsis-associated intestinal leakage in the gut is largely regulated by leukocyte accumulation. Thus, our novel data demonstrate a critical link between P-selectin-dependent leukocyte recruitment and gut barrier failure in endotoxemia.},
  author       = {Mangell, Peter and Röme, Andrada and Wang, Yusheng and Schramm, R and Jeppsson, Bengt and Thorlacius, Henrik},
  issn         = {1420-908X},
  keyword      = {P-selectin,leukocyte,sepsis,intestinal barrier,endotoxin},
  language     = {eng},
  number       = {5},
  pages        = {189--194},
  publisher    = {Birkhaüser},
  series       = {Inflammation Research},
  title        = {Critical role of P-selectin-dependent leukocyte recruitment in endotoxin-induced intestinal barrier dysfunction in mice.},
  url          = {http://dx.doi.org/10.1007/s00011-007-6163-x},
  volume       = {56},
  year         = {2007},
}