Critical role of P-selectin-dependent leukocyte recruitment in endotoxin-induced intestinal barrier dysfunction in mice.
(2007) In Inflammation Research 56(5). p.189-194- Abstract
- Objective: To define the importance of leukocyte recruitment in endotoxin-induced gut permeability. Materials and methods: 31 male C57BL/6 mice were challenged with lipopolysaccharide (LPS). Ileal permeability was measured in Ussing chambers and leukocyte-endothelium interactions studied with intravital fluorescence microscopy after 18 h. Results: LPS caused a clear-cut increase in leukocyte accumulation and intestinal permeability. Immunoneutralisation of P-selectin not only reduced leukocyte recruitment significantly (54% reduction) but also abolished endotoxin-induced intestinal leakage. Intestinal levels of pro-inflammatory chemokines increased markedly in response to LPS but were not influenced by inhibition of P-selectin in vivo.... (More)
- Objective: To define the importance of leukocyte recruitment in endotoxin-induced gut permeability. Materials and methods: 31 male C57BL/6 mice were challenged with lipopolysaccharide (LPS). Ileal permeability was measured in Ussing chambers and leukocyte-endothelium interactions studied with intravital fluorescence microscopy after 18 h. Results: LPS caused a clear-cut increase in leukocyte accumulation and intestinal permeability. Immunoneutralisation of P-selectin not only reduced leukocyte recruitment significantly (54% reduction) but also abolished endotoxin-induced intestinal leakage. Intestinal levels of pro-inflammatory chemokines increased markedly in response to LPS but were not influenced by inhibition of P-selectin in vivo. Conclusion: The present study shows not only that endotoxin-induced leukocyte recruitment is mediated by P-selectin but also that sepsis-associated intestinal leakage in the gut is largely regulated by leukocyte accumulation. Thus, our novel data demonstrate a critical link between P-selectin-dependent leukocyte recruitment and gut barrier failure in endotoxemia. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/539596
- author
- Mangell, Peter LU ; Röme, Andrada LU ; Wang, Yusheng LU ; Schramm, R ; Jeppsson, Bengt LU and Thorlacius, Henrik LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- P-selectin, leukocyte, sepsis, intestinal barrier, endotoxin
- in
- Inflammation Research
- volume
- 56
- issue
- 5
- pages
- 189 - 194
- publisher
- Birkhäuser
- external identifiers
-
- wos:000246804400003
- scopus:34250890739
- ISSN
- 1420-908X
- DOI
- 10.1007/s00011-007-6163-x
- language
- English
- LU publication?
- yes
- id
- 175813ce-128b-4ae9-baa9-1d2595b0d121 (old id 539596)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17588134&dopt=Abstract
- date added to LUP
- 2016-04-01 11:48:40
- date last changed
- 2024-01-07 21:25:28
@article{175813ce-128b-4ae9-baa9-1d2595b0d121, abstract = {{Objective: To define the importance of leukocyte recruitment in endotoxin-induced gut permeability. Materials and methods: 31 male C57BL/6 mice were challenged with lipopolysaccharide (LPS). Ileal permeability was measured in Ussing chambers and leukocyte-endothelium interactions studied with intravital fluorescence microscopy after 18 h. Results: LPS caused a clear-cut increase in leukocyte accumulation and intestinal permeability. Immunoneutralisation of P-selectin not only reduced leukocyte recruitment significantly (54% reduction) but also abolished endotoxin-induced intestinal leakage. Intestinal levels of pro-inflammatory chemokines increased markedly in response to LPS but were not influenced by inhibition of P-selectin in vivo. Conclusion: The present study shows not only that endotoxin-induced leukocyte recruitment is mediated by P-selectin but also that sepsis-associated intestinal leakage in the gut is largely regulated by leukocyte accumulation. Thus, our novel data demonstrate a critical link between P-selectin-dependent leukocyte recruitment and gut barrier failure in endotoxemia.}}, author = {{Mangell, Peter and Röme, Andrada and Wang, Yusheng and Schramm, R and Jeppsson, Bengt and Thorlacius, Henrik}}, issn = {{1420-908X}}, keywords = {{P-selectin; leukocyte; sepsis; intestinal barrier; endotoxin}}, language = {{eng}}, number = {{5}}, pages = {{189--194}}, publisher = {{Birkhäuser}}, series = {{Inflammation Research}}, title = {{Critical role of P-selectin-dependent leukocyte recruitment in endotoxin-induced intestinal barrier dysfunction in mice.}}, url = {{https://lup.lub.lu.se/search/files/2652337/626008.pdf}}, doi = {{10.1007/s00011-007-6163-x}}, volume = {{56}}, year = {{2007}}, }