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Non-contact acoustic capture of microparticles from small plasma volumes.

Evander, Mikael LU ; Gidlöf, Olof LU ; Olde, Björn LU ; Erlinge, David LU and Laurell, Thomas LU (2015) In Lab on A Chip 15(12). p.2588-2596
Abstract
Microparticles (MP) are small (100-1000 nm) membrane vesicles shed by cells as a response to activation, stress or apoptosis. Platelet-derived MP (PMP) has been shown to reflect the pathophysiological processes of a range of cardiovascular diseases and there is a potential clinical value in using PMPs as biomarkers, as well as a need to better understand the biology of these vesicles. The current method for isolating MP depends on differential centrifugation steps, which require relatively large sample volumes and have been shown to compromise the integrity and composition of the MP population. We present a novel method for rapid, non-contact capture of PMP in minute sample volumes based on a microscale acoustic standing wave technology.... (More)
Microparticles (MP) are small (100-1000 nm) membrane vesicles shed by cells as a response to activation, stress or apoptosis. Platelet-derived MP (PMP) has been shown to reflect the pathophysiological processes of a range of cardiovascular diseases and there is a potential clinical value in using PMPs as biomarkers, as well as a need to better understand the biology of these vesicles. The current method for isolating MP depends on differential centrifugation steps, which require relatively large sample volumes and have been shown to compromise the integrity and composition of the MP population. We present a novel method for rapid, non-contact capture of PMP in minute sample volumes based on a microscale acoustic standing wave technology. Capture of PMPs from plasma is shown by scanning electron microscopy and flow cytometry. Furthermore, the system is characterized with regards to plasma sample concentration and flow rate. Finally, the technique is compared to a standard differential centrifugation protocol using samples from both healthy controls and ST-elevation myocardial infarction (STEMI) patient samples. The acoustic system is shown to offer a quick and automated setup for extracting microparticles from small sample volumes with higher recovery than a standard differential centrifugation protocol. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Lab on A Chip
volume
15
issue
12
pages
2588 - 2596
publisher
Royal Society of Chemistry
external identifiers
  • pmid:25943791
  • wos:000355635200007
  • scopus:84930936167
ISSN
1473-0189
DOI
10.1039/c5lc00290g
language
English
LU publication?
yes
id
534c9801-c565-4339-b083-5a70c2b8b5e2 (old id 5456904)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25943791?dopt=Abstract
date added to LUP
2015-06-02 15:20:55
date last changed
2017-11-05 03:08:47
@article{534c9801-c565-4339-b083-5a70c2b8b5e2,
  abstract     = {Microparticles (MP) are small (100-1000 nm) membrane vesicles shed by cells as a response to activation, stress or apoptosis. Platelet-derived MP (PMP) has been shown to reflect the pathophysiological processes of a range of cardiovascular diseases and there is a potential clinical value in using PMPs as biomarkers, as well as a need to better understand the biology of these vesicles. The current method for isolating MP depends on differential centrifugation steps, which require relatively large sample volumes and have been shown to compromise the integrity and composition of the MP population. We present a novel method for rapid, non-contact capture of PMP in minute sample volumes based on a microscale acoustic standing wave technology. Capture of PMPs from plasma is shown by scanning electron microscopy and flow cytometry. Furthermore, the system is characterized with regards to plasma sample concentration and flow rate. Finally, the technique is compared to a standard differential centrifugation protocol using samples from both healthy controls and ST-elevation myocardial infarction (STEMI) patient samples. The acoustic system is shown to offer a quick and automated setup for extracting microparticles from small sample volumes with higher recovery than a standard differential centrifugation protocol.},
  author       = {Evander, Mikael and Gidlöf, Olof and Olde, Björn and Erlinge, David and Laurell, Thomas},
  issn         = {1473-0189},
  language     = {eng},
  number       = {12},
  pages        = {2588--2596},
  publisher    = {Royal Society of Chemistry},
  series       = {Lab on A Chip},
  title        = {Non-contact acoustic capture of microparticles from small plasma volumes.},
  url          = {http://dx.doi.org/10.1039/c5lc00290g},
  volume       = {15},
  year         = {2015},
}