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Iron supplements containing Lactobacillus plantarum 299v increase ferric iron and up-regulate the ferric reductase DCYTB in human Caco-2/HT29 MTX co-cultures

Sandberg, Ann Sofie; Önning, Gunilla LU ; Engström, Niklas and Scheers, Nathalie (2018) In Nutrients 10(12).
Abstract

Several human interventions have indicated that Lactobacillus plantarum 299v (L. plantarum 299v) increases intestinal iron absorption. The aim of the present study was to investigate possible effects of L. plantarum 299v on the mechanisms of iron absorption on the cellular level. We have previously shown that lactic fermentation of vegetables increased iron absorption in humans. It was revealed that the level of ferric iron [Fe (H2O)5]2+ was increased after fermentation. Therefore, we used voltammetry to measure the oxidation state of iron in simulated gastrointestinal digested oat and mango drinks and capsule meals containing L. plantarum 299v. We also exposed human intestinal co-cultures of enterocytes... (More)

Several human interventions have indicated that Lactobacillus plantarum 299v (L. plantarum 299v) increases intestinal iron absorption. The aim of the present study was to investigate possible effects of L. plantarum 299v on the mechanisms of iron absorption on the cellular level. We have previously shown that lactic fermentation of vegetables increased iron absorption in humans. It was revealed that the level of ferric iron [Fe (H2O)5]2+ was increased after fermentation. Therefore, we used voltammetry to measure the oxidation state of iron in simulated gastrointestinal digested oat and mango drinks and capsule meals containing L. plantarum 299v. We also exposed human intestinal co-cultures of enterocytes and goblet cells (Caco-2/HT29 MTX) to the supplements in order to study the effect on proteins possibly involved (MUC5AC, DCYTB, DMT1, and ferritin). We detected an increase in ferric iron in the digested meals and drinks containing L. plantarum 299v. In the intestinal cell model, we observed that the ferric reductase DCYTB increased in the presence of L. plantarum 299v, while the production of mucin (MUC5AC) decreased independently of L. plantarum 299v. In conclusion, the data suggest that the effect of L. plantarum 299v on iron metabolism is mediated through driving the Fe3+/DCYTB axis.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
DCYTB, DMT1, DSM9843, Iron, Lactobacillus plantarum 299v, MUC5AC
in
Nutrients
volume
10
issue
12
publisher
MDPI AG
external identifiers
  • scopus:85058319011
ISSN
2072-6643
DOI
10.3390/nu10121949
language
English
LU publication?
yes
id
54a8c868-9d6c-41f3-8350-5c8ebf8c6db2
date added to LUP
2019-01-04 08:23:39
date last changed
2019-02-20 11:41:34
@article{54a8c868-9d6c-41f3-8350-5c8ebf8c6db2,
  abstract     = {<p>Several human interventions have indicated that Lactobacillus plantarum 299v (L. plantarum 299v) increases intestinal iron absorption. The aim of the present study was to investigate possible effects of L. plantarum 299v on the mechanisms of iron absorption on the cellular level. We have previously shown that lactic fermentation of vegetables increased iron absorption in humans. It was revealed that the level of ferric iron [Fe (H<sub>2</sub>O)<sub>5</sub>]<sup>2+</sup> was increased after fermentation. Therefore, we used voltammetry to measure the oxidation state of iron in simulated gastrointestinal digested oat and mango drinks and capsule meals containing L. plantarum 299v. We also exposed human intestinal co-cultures of enterocytes and goblet cells (Caco-2/HT29 MTX) to the supplements in order to study the effect on proteins possibly involved (MUC5AC, DCYTB, DMT1, and ferritin). We detected an increase in ferric iron in the digested meals and drinks containing L. plantarum 299v. In the intestinal cell model, we observed that the ferric reductase DCYTB increased in the presence of L. plantarum 299v, while the production of mucin (MUC5AC) decreased independently of L. plantarum 299v. In conclusion, the data suggest that the effect of L. plantarum 299v on iron metabolism is mediated through driving the Fe<sup>3+</sup>/DCYTB axis.</p>},
  articleno    = {1949},
  author       = {Sandberg, Ann Sofie and Önning, Gunilla and Engström, Niklas and Scheers, Nathalie},
  issn         = {2072-6643},
  keyword      = {DCYTB,DMT1,DSM9843,Iron,Lactobacillus plantarum 299v,MUC5AC},
  language     = {eng},
  month        = {12},
  number       = {12},
  publisher    = {MDPI AG},
  series       = {Nutrients},
  title        = {Iron supplements containing Lactobacillus plantarum 299v increase ferric iron and up-regulate the ferric reductase DCYTB in human Caco-2/HT29 MTX co-cultures},
  url          = {http://dx.doi.org/10.3390/nu10121949},
  volume       = {10},
  year         = {2018},
}