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Suppression of external NADPH dehydrogenase—NDB1 in arabidopsis thaliana confers improved tolerance to ammonium toxicity via efficient Glutathione/Redox metabolism

Podgórska, Anna; Ostaszewska-Bugajska, Monika; Borysiuk, Klaudia; Tarnowska, Agata; Jakubiak, Monika; Burian, Maria; Rasmusson, Allan G. LU and Szal, Bożena (2018) In International Journal of Molecular Sciences 19(5).
Abstract

Environmental stresses, including ammonium (NH4 +) nourishment, can damage key mitochondrial components through the production of surplus reactive oxygen species (ROS) in the mitochondrial electron transport chain. However, alternative electron pathways are significant for efficient reductant dissipation in mitochondria during ammonium nutrition. The aim of this study was to define the role of external NADPH-dehydrogenase (NDB1) during oxidative metabolism of NH4 +-fed plants. Most plant species grown with NH4 + as the sole nitrogen source experience a condition known as “ammonium toxicity syndrome”. Surprisingly, transgenic Arabidopsis thaliana plants suppressing NDB1... (More)

Environmental stresses, including ammonium (NH4 +) nourishment, can damage key mitochondrial components through the production of surplus reactive oxygen species (ROS) in the mitochondrial electron transport chain. However, alternative electron pathways are significant for efficient reductant dissipation in mitochondria during ammonium nutrition. The aim of this study was to define the role of external NADPH-dehydrogenase (NDB1) during oxidative metabolism of NH4 +-fed plants. Most plant species grown with NH4 + as the sole nitrogen source experience a condition known as “ammonium toxicity syndrome”. Surprisingly, transgenic Arabidopsis thaliana plants suppressing NDB1 were more resistant to NH4 + treatment. The NDB1 knock-down line was characterized by milder oxidative stress symptoms in plant tissues when supplied with NH4 +. Mitochondrial ROS accumulation, in particular, was attenuated in the NDB1 knock-down plants during NH4 + treatment. Enhanced antioxidant defense, primarily concerning the glutathione pool, may prevent ROS accumulation in NH4 +-grown NDB1-suppressing plants. We found that induction of glutathione peroxidase-like enzymes and peroxiredoxins in the NDB1-surpressing line contributed to lower ammonium-toxicity stress. The major conclusion of this study was that NDB1 suppression in plants confers tolerance to changes in redox homeostasis that occur in response to prolonged ammonium nutrition, causing cross tolerance among plants.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Ammonium toxicity, External type II NADPH dehydrogenase, Glutathione metabolism, Reactive oxygen species, Redox homeostasis
in
International Journal of Molecular Sciences
volume
19
issue
5
publisher
MOLECULAR DIVERSITY PRESERVATION INT
external identifiers
  • scopus:85046792909
ISSN
1661-6596
DOI
10.3390/ijms19051412
language
English
LU publication?
yes
id
557c1ae8-d7d0-4f13-a558-bf63bec1c5e8
date added to LUP
2018-05-23 14:53:10
date last changed
2018-10-03 12:04:15
@article{557c1ae8-d7d0-4f13-a558-bf63bec1c5e8,
  abstract     = {<p>Environmental stresses, including ammonium (NH<sub>4</sub> <sup>+</sup>) nourishment, can damage key mitochondrial components through the production of surplus reactive oxygen species (ROS) in the mitochondrial electron transport chain. However, alternative electron pathways are significant for efficient reductant dissipation in mitochondria during ammonium nutrition. The aim of this study was to define the role of external NADPH-dehydrogenase (NDB1) during oxidative metabolism of NH<sub>4</sub> <sup>+</sup>-fed plants. Most plant species grown with NH<sub>4</sub> <sup>+</sup> as the sole nitrogen source experience a condition known as “ammonium toxicity syndrome”. Surprisingly, transgenic Arabidopsis thaliana plants suppressing NDB1 were more resistant to NH<sub>4</sub> <sup>+</sup> treatment. The NDB1 knock-down line was characterized by milder oxidative stress symptoms in plant tissues when supplied with NH<sub>4</sub> <sup>+</sup>. Mitochondrial ROS accumulation, in particular, was attenuated in the NDB1 knock-down plants during NH<sub>4</sub> <sup>+</sup> treatment. Enhanced antioxidant defense, primarily concerning the glutathione pool, may prevent ROS accumulation in NH<sub>4</sub> <sup>+</sup>-grown NDB1-suppressing plants. We found that induction of glutathione peroxidase-like enzymes and peroxiredoxins in the NDB1-surpressing line contributed to lower ammonium-toxicity stress. The major conclusion of this study was that NDB1 suppression in plants confers tolerance to changes in redox homeostasis that occur in response to prolonged ammonium nutrition, causing cross tolerance among plants.</p>},
  articleno    = {1412},
  author       = {Podgórska, Anna and Ostaszewska-Bugajska, Monika and Borysiuk, Klaudia and Tarnowska, Agata and Jakubiak, Monika and Burian, Maria and Rasmusson, Allan G. and Szal, Bożena},
  issn         = {1661-6596},
  keyword      = {Ammonium toxicity,External type II NADPH dehydrogenase,Glutathione metabolism,Reactive oxygen species,Redox homeostasis},
  language     = {eng},
  month        = {05},
  number       = {5},
  publisher    = {MOLECULAR DIVERSITY PRESERVATION INT},
  series       = {International Journal of Molecular Sciences},
  title        = {Suppression of external NADPH dehydrogenase—NDB1 in arabidopsis thaliana confers improved tolerance to ammonium toxicity via efficient Glutathione/Redox metabolism},
  url          = {http://dx.doi.org/10.3390/ijms19051412},
  volume       = {19},
  year         = {2018},
}