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Helicobacter pylori outer membrane vesicles protect the pathogen from reactive oxygen species of the respiratory burst

Lekmeechai, Sujinna LU ; Su, Yu Ching; Brant, Marta LU ; Alvarado-Kristensson, Maria LU ; Vallström, Anna; Obi, Ikenna; Arnqvist, Anna and Riesbeck, Kristian LU (2018) In Frontiers in Microbiology 9(SEP).
Abstract

Outer membrane vesicles (OMVs) play an important role in the persistence of Helicobacter pylori infection. Helicobacter OMVs carry a plethora of virulence factors, including catalase (KatA), an antioxidant enzyme that counteracts the host respiratory burst. We found KatA to be enriched and surface-associated in OMVs compared to bacterial cells. This conferred OMV-dependent KatA activity resulting in neutralization of H2O2 and NaClO, and rescue of surrounding bacteria from oxidative damage. The antioxidant activity of OMVs was abolished by deletion of KatA. In conclusion, enrichment of antioxidative KatA in OMVs is highly important for efficient immune evasion.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
H. pylori, KatA, Outer membrane vesicles, Oxidative burst, Reactive oxygen species
in
Frontiers in Microbiology
volume
9
issue
SEP
publisher
Frontiers
external identifiers
  • scopus:85053053080
ISSN
1664-302X
DOI
10.3389/fmicb.2018.01837
language
English
LU publication?
yes
id
55a66bb9-17ca-4e7a-b4cf-510f7e3c4f8c
date added to LUP
2018-10-12 07:47:47
date last changed
2019-02-20 11:31:13
@article{55a66bb9-17ca-4e7a-b4cf-510f7e3c4f8c,
  abstract     = {<p>Outer membrane vesicles (OMVs) play an important role in the persistence of Helicobacter pylori infection. Helicobacter OMVs carry a plethora of virulence factors, including catalase (KatA), an antioxidant enzyme that counteracts the host respiratory burst. We found KatA to be enriched and surface-associated in OMVs compared to bacterial cells. This conferred OMV-dependent KatA activity resulting in neutralization of H<sub>2</sub>O<sub>2</sub> and NaClO, and rescue of surrounding bacteria from oxidative damage. The antioxidant activity of OMVs was abolished by deletion of KatA. In conclusion, enrichment of antioxidative KatA in OMVs is highly important for efficient immune evasion.</p>},
  articleno    = {1837},
  author       = {Lekmeechai, Sujinna and Su, Yu Ching and Brant, Marta and Alvarado-Kristensson, Maria and Vallström, Anna and Obi, Ikenna and Arnqvist, Anna and Riesbeck, Kristian},
  issn         = {1664-302X},
  keyword      = {H. pylori,KatA,Outer membrane vesicles,Oxidative burst,Reactive oxygen species},
  language     = {eng},
  month        = {09},
  number       = {SEP},
  publisher    = {Frontiers},
  series       = {Frontiers in Microbiology},
  title        = {Helicobacter pylori outer membrane vesicles protect the pathogen from reactive oxygen species of the respiratory burst},
  url          = {http://dx.doi.org/10.3389/fmicb.2018.01837},
  volume       = {9},
  year         = {2018},
}