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Filter-exchange PGSE NMR determination of cell membrane permeability.

Åslund, Ingrid LU ; Nowacka, Agnieszka LU ; Nilsson, Markus LU and Topgaard, Daniel LU (2009) In Journal of Magnetic Resonance 200. p.291-295
Abstract
A new PGSE NMR sequence is introduced for measuring diffusive transport across the plasma membrane of living cells. A "diffusion filter" and a variable mixing time precedes a standard PGSE block for diffusion encoding of the NMR signal. The filter is a PGSE block optimized for selectively removing the magnetization of the extracellular water. With increasing mixing time the intra- and extracellular components approach their equilibrium fractional populations. The rate of exchange can be measured using only a few minutes of instrument time. Water exchange over the plasma membrane of starved yeast cells is studied in the temperature range +5 to +32 degrees C.
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Magnetic Resonance
volume
200
pages
291 - 295
publisher
Academic Press
external identifiers
  • wos:000272260900016
  • pmid:19647458
  • scopus:69949132949
  • pmid:19647458
ISSN
1096-0856
DOI
10.1016/j.jmr.2009.07.015
language
English
LU publication?
yes
id
55c69fc1-6eb9-41cc-bb4b-dba3beb1682e (old id 1470185)
date added to LUP
2016-04-01 13:35:31
date last changed
2022-03-21 19:24:55
@article{55c69fc1-6eb9-41cc-bb4b-dba3beb1682e,
  abstract     = {{A new PGSE NMR sequence is introduced for measuring diffusive transport across the plasma membrane of living cells. A "diffusion filter" and a variable mixing time precedes a standard PGSE block for diffusion encoding of the NMR signal. The filter is a PGSE block optimized for selectively removing the magnetization of the extracellular water. With increasing mixing time the intra- and extracellular components approach their equilibrium fractional populations. The rate of exchange can be measured using only a few minutes of instrument time. Water exchange over the plasma membrane of starved yeast cells is studied in the temperature range +5 to +32 degrees C.}},
  author       = {{Åslund, Ingrid and Nowacka, Agnieszka and Nilsson, Markus and Topgaard, Daniel}},
  issn         = {{1096-0856}},
  language     = {{eng}},
  pages        = {{291--295}},
  publisher    = {{Academic Press}},
  series       = {{Journal of Magnetic Resonance}},
  title        = {{Filter-exchange PGSE NMR determination of cell membrane permeability.}},
  url          = {{http://dx.doi.org/10.1016/j.jmr.2009.07.015}},
  doi          = {{10.1016/j.jmr.2009.07.015}},
  volume       = {{200}},
  year         = {{2009}},
}