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Proteins and antibodies in serum, plasma, and whole blood—size characterization using asymmetrical flow field-flow fractionation (AF4)

Leeman, Mats LU ; Choi, Jaeyeong LU ; Hansson, Sebastian LU ; Storm, Matilda Ulmius and Nilsson, Lars LU (2018) In Analytical and Bioanalytical Chemistry p.1-7
Abstract

The analysis of aggregates of therapeutic proteins is crucial in order to ensure efficacy and patient safety. Typically, the analysis is performed in the finished formulation to ensure that aggregates are not present. An important question is, however, what happens to therapeutic proteins, with regard to oligomerization and aggregation, after they have been administrated (i.e., in the blood). In this paper, the separation of whole blood, plasma, and serum is shown using asymmetric flow field-flow fractionation (AF4) with a minimum of sample pre-treatment. Furthermore, the analysis and size characterization of a fluorescent antibody in blood plasma using AF4 are demonstrated. The results show the suitability and strength of AF4 for blood... (More)

The analysis of aggregates of therapeutic proteins is crucial in order to ensure efficacy and patient safety. Typically, the analysis is performed in the finished formulation to ensure that aggregates are not present. An important question is, however, what happens to therapeutic proteins, with regard to oligomerization and aggregation, after they have been administrated (i.e., in the blood). In this paper, the separation of whole blood, plasma, and serum is shown using asymmetric flow field-flow fractionation (AF4) with a minimum of sample pre-treatment. Furthermore, the analysis and size characterization of a fluorescent antibody in blood plasma using AF4 are demonstrated. The results show the suitability and strength of AF4 for blood analysis and open new important routes for the analysis and characterization of therapeutic proteins in the blood.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
Antibodies, Asymmetric flow field-flow fractionation (AF4), Fluorescence labelling, Plasma, Serum, Whole blood
in
Analytical and Bioanalytical Chemistry
pages
7 pages
publisher
Springer
external identifiers
  • scopus:85047660690
ISSN
1618-2642
DOI
10.1007/s00216-018-1127-2
language
English
LU publication?
yes
id
56cf8c7a-35be-4273-bee6-ceb799ef3df9
date added to LUP
2018-06-12 15:46:44
date last changed
2018-11-21 21:40:18
@article{56cf8c7a-35be-4273-bee6-ceb799ef3df9,
  abstract     = {<p>The analysis of aggregates of therapeutic proteins is crucial in order to ensure efficacy and patient safety. Typically, the analysis is performed in the finished formulation to ensure that aggregates are not present. An important question is, however, what happens to therapeutic proteins, with regard to oligomerization and aggregation, after they have been administrated (i.e., in the blood). In this paper, the separation of whole blood, plasma, and serum is shown using asymmetric flow field-flow fractionation (AF4) with a minimum of sample pre-treatment. Furthermore, the analysis and size characterization of a fluorescent antibody in blood plasma using AF4 are demonstrated. The results show the suitability and strength of AF4 for blood analysis and open new important routes for the analysis and characterization of therapeutic proteins in the blood.</p>},
  author       = {Leeman, Mats and Choi, Jaeyeong and Hansson, Sebastian and Storm, Matilda Ulmius and Nilsson, Lars},
  issn         = {1618-2642},
  keyword      = {Antibodies,Asymmetric flow field-flow fractionation (AF4),Fluorescence labelling,Plasma,Serum,Whole blood},
  language     = {eng},
  month        = {05},
  pages        = {1--7},
  publisher    = {Springer},
  series       = {Analytical and Bioanalytical Chemistry},
  title        = {Proteins and antibodies in serum, plasma, and whole blood—size characterization using asymmetrical flow field-flow fractionation (AF4)},
  url          = {http://dx.doi.org/10.1007/s00216-018-1127-2},
  year         = {2018},
}