Fluid and Highly Curved Model Membranes on Vertical Nanowire Arrays
Dabkowska, Aleksandra LU ; Niman, Cassandra LU ; Piret, Gaelle LU ; Persson, Henrik LU ; Wacklin, Hanna LU
; Linke, Heiner
LU
; Prinz, Christelle
LU
and Nylander, Tommy
LU
(2014)
In Nano Letters
14(8).
p.4286-4292
- Abstract
- Sensing and manipulating living cells using vertical nanowire devices requires a complete understanding of cell behavior on these substrates. Changes in cell function and phenotype are often triggered by events taking place at the plasma membrane, the properties of which are influenced by local curvature. The nanowire topography can therefore be expected to greatly affect the cell membrane, emphasizing the importance of studying membranes on vertical nanowire arrays. Here, we used supported phospholipid bilayers as a model for biomembranes. We demonstrate the formation of fluid supported bilayers on vertical nanowire forests using self-assembly from vesicles in solution. The bilayers were found to follow the contours of the nanowires to... (More)
- Sensing and manipulating living cells using vertical nanowire devices requires a complete understanding of cell behavior on these substrates. Changes in cell function and phenotype are often triggered by events taking place at the plasma membrane, the properties of which are influenced by local curvature. The nanowire topography can therefore be expected to greatly affect the cell membrane, emphasizing the importance of studying membranes on vertical nanowire arrays. Here, we used supported phospholipid bilayers as a model for biomembranes. We demonstrate the formation of fluid supported bilayers on vertical nanowire forests using self-assembly from vesicles in solution. The bilayers were found to follow the contours of the nanowires to form continuous and locally highly curved model membranes. Distinct from standard flat supported lipid bilayers, the high aspect ratio of the nanowires results in a large bilayer surface available for the immobilization and study of biomolecules. We used these bilayers to bind a membrane-anchored protein as well as tethered vesicles on the nanowire substrate. The nanowire-bilayer platform shown here can be expanded from fundamental studies of lipid membranes on controlled curvature substrates to the development of innovative membrane-based nanosensors. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4652964
- author
- Dabkowska, Aleksandra
LU
; Niman, Cassandra
LU
; Piret, Gaelle
LU
; Persson, Henrik
LU
; Wacklin, Hanna
LU
; Linke, Heiner
LU
; Prinz, Christelle
LU
and Nylander, Tommy
LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Nanowires, lipid bilayer, membrane curvature, protein-lipid interaction, nanotopography, nanostructure
- in
- Nano Letters
- volume
- 14
- issue
- 8
- pages
- 4286 - 4292
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000340446200014
- scopus:84906091016
- pmid:24971634
- ISSN
- 1530-6992
- DOI
- 10.1021/nl500926y
- language
- English
- LU publication?
- yes
- id
- 56fc2bc2-f069-4e3d-b57e-dc9807e1630b (old id 4652964)
- date added to LUP
- 2016-04-01 14:55:18
- date last changed
- 2023-11-13 14:00:31
@article{56fc2bc2-f069-4e3d-b57e-dc9807e1630b,
abstract = {{Sensing and manipulating living cells using vertical nanowire devices requires a complete understanding of cell behavior on these substrates. Changes in cell function and phenotype are often triggered by events taking place at the plasma membrane, the properties of which are influenced by local curvature. The nanowire topography can therefore be expected to greatly affect the cell membrane, emphasizing the importance of studying membranes on vertical nanowire arrays. Here, we used supported phospholipid bilayers as a model for biomembranes. We demonstrate the formation of fluid supported bilayers on vertical nanowire forests using self-assembly from vesicles in solution. The bilayers were found to follow the contours of the nanowires to form continuous and locally highly curved model membranes. Distinct from standard flat supported lipid bilayers, the high aspect ratio of the nanowires results in a large bilayer surface available for the immobilization and study of biomolecules. We used these bilayers to bind a membrane-anchored protein as well as tethered vesicles on the nanowire substrate. The nanowire-bilayer platform shown here can be expanded from fundamental studies of lipid membranes on controlled curvature substrates to the development of innovative membrane-based nanosensors.}},
author = {{Dabkowska, Aleksandra and Niman, Cassandra and Piret, Gaelle and Persson, Henrik and Wacklin, Hanna and Linke, Heiner and Prinz, Christelle and Nylander, Tommy}},
issn = {{1530-6992}},
keywords = {{Nanowires; lipid bilayer; membrane curvature; protein-lipid interaction; nanotopography; nanostructure}},
language = {{eng}},
number = {{8}},
pages = {{4286--4292}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Nano Letters}},
title = {{Fluid and Highly Curved Model Membranes on Vertical Nanowire Arrays}},
url = {{http://dx.doi.org/10.1021/nl500926y}},
doi = {{10.1021/nl500926y}},
volume = {{14}},
year = {{2014}},
}