Suppression of T-Cell Proliferation by Normal Density Granulocytes Led to CD183 Downregulation and Cytokine Inhibition in T-Cells
Westerlund, Julia LU ; Askman, Sandra LU ; Pettersson, Åsa LU ; Hellmark, Thomas LU
; Johansson, Åsa C M
LU
and Hansson, Markus
LU
(2022)
In Journal of Immunology Research
2022.
- Abstract
Normal density granulocytes (NDGs) can suppress T-cell responses in a similar way as myeloid-derived suppressor cells (MDSCs). In this study, we tested the hypothesis that NDGs from healthy donors preferentially inhibit T helper 1 (Th1) cells and investigated the myeloid-derived suppressive effect in different T-cell populations. We found that NDG-induced suppression of T-cell proliferation was contact dependent, mediated by integrin CD11b, and dependent on NDG-production of reactive oxygen species (ROS). The suppression was rapid and occurred within the first few hours of coculture. The suppression did not influence the CD8
+/CD4
+ ratio indicating an equal sensitivity in these populations. We further analyzed the CD4
+... (More)Normal density granulocytes (NDGs) can suppress T-cell responses in a similar way as myeloid-derived suppressor cells (MDSCs). In this study, we tested the hypothesis that NDGs from healthy donors preferentially inhibit T helper 1 (Th1) cells and investigated the myeloid-derived suppressive effect in different T-cell populations. We found that NDG-induced suppression of T-cell proliferation was contact dependent, mediated by integrin CD11b, and dependent on NDG-production of reactive oxygen species (ROS). The suppression was rapid and occurred within the first few hours of coculture. The suppression did not influence the CD8
(Less)
+/CD4
+ ratio indicating an equal sensitivity in these populations. We further analyzed the CD4
+ T helper subsets and found that NDGs induced a loss of Th1 surface marker, CD183, that was unrelated to ligand-binding to CD183. In addition, we analyzed the Th1, Th2, and Th17 cytokine production and found that all cytokine groups were suppressed when T-cells were incubated with NDGs. We therefore concluded that NDGs do not preferentially suppress Th1-cells. Instead, NDGs generally suppress Th cells and cytotoxic T-cells but specifically downregulate the Th1 marker CD183.
- author
- Westerlund, Julia
LU
; Askman, Sandra
LU
; Pettersson, Åsa
LU
; Hellmark, Thomas
LU
; Johansson, Åsa C M
LU
and Hansson, Markus
LU
- organization
- publishing date
- 2022
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cytokines, Down-Regulation, Lymphocyte Activation, Cell Proliferation, Granulocytes
- in
- Journal of Immunology Research
- volume
- 2022
- article number
- 8077281
- publisher
- Hindawi Limited
- external identifiers
-
- scopus:85142802638
- pmid:36438199
- ISSN
- 2314-7156
- DOI
- 10.1155/2022/8077281
- language
- English
- LU publication?
- yes
- additional info
- Copyright © 2022 Julia Westerlund et al.
- id
- 571ec9b7-586a-49a8-ad28-9a80901f3836
- date added to LUP
- 2022-12-07 16:35:55
- date last changed
- 2024-06-13 21:20:57
@article{571ec9b7-586a-49a8-ad28-9a80901f3836,
abstract = {{<p>Normal density granulocytes (NDGs) can suppress T-cell responses in a similar way as myeloid-derived suppressor cells (MDSCs). In this study, we tested the hypothesis that NDGs from healthy donors preferentially inhibit T helper 1 (Th1) cells and investigated the myeloid-derived suppressive effect in different T-cell populations. We found that NDG-induced suppression of T-cell proliferation was contact dependent, mediated by integrin CD11b, and dependent on NDG-production of reactive oxygen species (ROS). The suppression was rapid and occurred within the first few hours of coculture. The suppression did not influence the CD8<br>
+/CD4<br>
+ ratio indicating an equal sensitivity in these populations. We further analyzed the CD4<br>
+ T helper subsets and found that NDGs induced a loss of Th1 surface marker, CD183, that was unrelated to ligand-binding to CD183. In addition, we analyzed the Th1, Th2, and Th17 cytokine production and found that all cytokine groups were suppressed when T-cells were incubated with NDGs. We therefore concluded that NDGs do not preferentially suppress Th1-cells. Instead, NDGs generally suppress Th cells and cytotoxic T-cells but specifically downregulate the Th1 marker CD183.<br>
</p>}},
author = {{Westerlund, Julia and Askman, Sandra and Pettersson, Åsa and Hellmark, Thomas and Johansson, Åsa C M and Hansson, Markus}},
issn = {{2314-7156}},
keywords = {{Cytokines; Down-Regulation; Lymphocyte Activation; Cell Proliferation; Granulocytes}},
language = {{eng}},
publisher = {{Hindawi Limited}},
series = {{Journal of Immunology Research}},
title = {{Suppression of T-Cell Proliferation by Normal Density Granulocytes Led to CD183 Downregulation and Cytokine Inhibition in T-Cells}},
url = {{http://dx.doi.org/10.1155/2022/8077281}},
doi = {{10.1155/2022/8077281}},
volume = {{2022}},
year = {{2022}},
}