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Hydrogen peroxide vapour treatment inactivates norovirus but has limited effect on post-treatment viral RNA levels

Holmdahl, Torsten LU ; Odenholt, Inga LU ; Riesbeck, Kristian LU ; Medstrand, Patrik LU and Widell, Anders LU (2019) In Infectious Diseases
Abstract

Background: Hydrogen peroxide vapour is used as a room disinfectant. Its activity against murine norovirus, a surrogate viability marker for human norovirus, indicates that it is also active against human norovirus. Aim: The aim of this study is to assess how this effect on viability is reflected in measurements of RNA by quantitative PCR (qPCR). Methods: Faeces suspensions of two human norovirus field strains, genogroup I and II and one cultured murine norovirus strain, (genogroup V) were dried on plastic plates, and underwent hydrogen peroxide vapour treatment or were mock treated. The influence of hydrogen peroxide on RNA was measured on genogroups I, II and V by qPCRs and for the cultivable murine norovirus also for viability by... (More)

Background: Hydrogen peroxide vapour is used as a room disinfectant. Its activity against murine norovirus, a surrogate viability marker for human norovirus, indicates that it is also active against human norovirus. Aim: The aim of this study is to assess how this effect on viability is reflected in measurements of RNA by quantitative PCR (qPCR). Methods: Faeces suspensions of two human norovirus field strains, genogroup I and II and one cultured murine norovirus strain, (genogroup V) were dried on plastic plates, and underwent hydrogen peroxide vapour treatment or were mock treated. The influence of hydrogen peroxide on RNA was measured on genogroups I, II and V by qPCRs and for the cultivable murine norovirus also for viability by cell culture. Virucidal activity on murine norovirus was measured by endpoint titrations as the 50% tissue culture infectious dose, based both on cytopathic effect and on presence of replicating intracellular minus strand RNA. Results: The mean impact on the human norovirus qPCRs was 0.4 log10. The murine norovirus qPCR changed by 1.7 log10 but by an alternative qPCR only by 0.4 log10. These minor changes contrasted the 4.5-5.0 log10 murine norovirus viability reduction after treatment measured by cytopathic effect or intracellular negative-strand RNA. Conclusion: Inactivation of murine norovirus viability by hydrogen peroxide vapour was not reflected in qPCR levels. This finding might be extrapolated to the related human norovirus genogroups. We further found that cellular minus strand murine norovirus PCR was an observer-independent marker to study reduction of murine norovirus viability.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
Hydrogen Peroxide, Minus-strand RNA, Murine norovirus, Norovirus, Surface decontamination
in
Infectious Diseases
publisher
Informa Healthcare
external identifiers
  • scopus:85060179562
ISSN
2374-4235
DOI
10.1080/23744235.2018.1546056
language
English
LU publication?
yes
id
58b6cf31-e1ad-49c4-8691-25c1960faa3f
date added to LUP
2019-02-05 15:12:47
date last changed
2019-02-20 11:48:11
@article{58b6cf31-e1ad-49c4-8691-25c1960faa3f,
  abstract     = {<p>Background: Hydrogen peroxide vapour is used as a room disinfectant. Its activity against murine norovirus, a surrogate viability marker for human norovirus, indicates that it is also active against human norovirus. Aim: The aim of this study is to assess how this effect on viability is reflected in measurements of RNA by quantitative PCR (qPCR). Methods: Faeces suspensions of two human norovirus field strains, genogroup I and II and one cultured murine norovirus strain, (genogroup V) were dried on plastic plates, and underwent hydrogen peroxide vapour treatment or were mock treated. The influence of hydrogen peroxide on RNA was measured on genogroups I, II and V by qPCRs and for the cultivable murine norovirus also for viability by cell culture. Virucidal activity on murine norovirus was measured by endpoint titrations as the 50% tissue culture infectious dose, based both on cytopathic effect and on presence of replicating intracellular minus strand RNA. Results: The mean impact on the human norovirus qPCRs was 0.4 log<sub>10</sub>. The murine norovirus qPCR changed by 1.7 log<sub>10</sub> but by an alternative qPCR only by 0.4 log<sub>10</sub>. These minor changes contrasted the 4.5-5.0 log<sub>10</sub> murine norovirus viability reduction after treatment measured by cytopathic effect or intracellular negative-strand RNA. Conclusion: Inactivation of murine norovirus viability by hydrogen peroxide vapour was not reflected in qPCR levels. This finding might be extrapolated to the related human norovirus genogroups. We further found that cellular minus strand murine norovirus PCR was an observer-independent marker to study reduction of murine norovirus viability.</p>},
  author       = {Holmdahl, Torsten and Odenholt, Inga and Riesbeck, Kristian and Medstrand, Patrik and Widell, Anders},
  issn         = {2374-4235},
  keyword      = {Hydrogen Peroxide,Minus-strand RNA,Murine norovirus,Norovirus,Surface decontamination},
  language     = {eng},
  publisher    = {Informa Healthcare},
  series       = {Infectious Diseases},
  title        = {Hydrogen peroxide vapour treatment inactivates norovirus but has limited effect on post-treatment viral RNA levels},
  url          = {http://dx.doi.org/10.1080/23744235.2018.1546056},
  year         = {2019},
}