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The Progression of Mycosis Fungoides During Treatment with Mogamulizumab: A BIO-MUSE Case Study of the Tumor and Immune Response in Peripheral Blood and Tissue

Johansson, Angelica LU orcid ; Kalliara, Eirini LU ; Belfrage, Emma LU ; Alling, Teodor LU ; Pyl, Paul Theodor LU ; Sandström Gerdtsson, Anna LU ; Gullberg, Urban LU ; Porwit, Anna LU ; Drott, Kristina LU and Ek, Sara LU orcid (2025) In Biomedicines 13. p.1-29
Abstract
Background/objectives: Mycosis fungoides (MF) is a rare malignancy, with an indolent course in the early stages of the disease. However, due to major molecular and clinical heterogeneity, patients at an advanced stage of the disease have variable responses to treatment and considerably reduced life expectancy. Today, there is a lack of specific markers for the progression from early to advanced stages of the disease. To address these challenges, the non-interventional BIO-MUSE trial was initiated. Here, we report on a case study involving one patient, where combined omics analysis of tissue and blood was used to reveal the unique molecular features associated with the progression of the disease. Methods: We applied 10× genomics-based... (More)
Background/objectives: Mycosis fungoides (MF) is a rare malignancy, with an indolent course in the early stages of the disease. However, due to major molecular and clinical heterogeneity, patients at an advanced stage of the disease have variable responses to treatment and considerably reduced life expectancy. Today, there is a lack of specific markers for the progression from early to advanced stages of the disease. To address these challenges, the non-interventional BIO-MUSE trial was initiated. Here, we report on a case study involving one patient, where combined omics analysis of tissue and blood was used to reveal the unique molecular features associated with the progression of the disease. Methods: We applied 10× genomics-based single-cell RNA sequencing to CD3+ peripheral T-cells, combined with T-cell receptor sequencing, to samples collected at multiple timepoints during the progression of the disease. In addition, GeoMx-based digital spatial profiling of T-helper (CD3+/CD8−), T-cytotoxic (CD3+/CD8+), and CD163+ cells was performed on skin biopsies. Results. The results pinpoint targets, such as transforming growth factor β1, as some of the mechanisms underlying disease progression, which may have the potential to improve patient prognostication and the development of precision medicine efforts. Conclusions: We propose that in patients with MF, the evolution of the malignant clone and the associated immune response need to be studied jointly to define relevant strategies for intervention. (Less)
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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomedicines
volume
13
article number
186
pages
1 - 29
publisher
MDPI AG
external identifiers
  • scopus:85215939769
  • pmid:39857770
ISSN
2227-9059
DOI
10.3390/biomedicines13010186
language
English
LU publication?
yes
id
59f8169a-5ba1-4373-91d9-1cf71a1ee12f
date added to LUP
2025-01-24 16:20:51
date last changed
2025-04-26 03:00:02
@article{59f8169a-5ba1-4373-91d9-1cf71a1ee12f,
  abstract     = {{Background/objectives: Mycosis fungoides (MF) is a rare malignancy, with an indolent course in the early stages of the disease. However, due to major molecular and clinical heterogeneity, patients at an advanced stage of the disease have variable responses to treatment and considerably reduced life expectancy. Today, there is a lack of specific markers for the progression from early to advanced stages of the disease. To address these challenges, the non-interventional BIO-MUSE trial was initiated. Here, we report on a case study involving one patient, where combined omics analysis of tissue and blood was used to reveal the unique molecular features associated with the progression of the disease. Methods: We applied 10× genomics-based single-cell RNA sequencing to CD3+ peripheral T-cells, combined with T-cell receptor sequencing, to samples collected at multiple timepoints during the progression of the disease. In addition, GeoMx-based digital spatial profiling of T-helper (CD3+/CD8−), T-cytotoxic (CD3+/CD8+), and CD163+ cells was performed on skin biopsies. Results. The results pinpoint targets, such as transforming growth factor β1, as some of the mechanisms underlying disease progression, which may have the potential to improve patient prognostication and the development of precision medicine efforts. Conclusions: We propose that in patients with MF, the evolution of the malignant clone and the associated immune response need to be studied jointly to define relevant strategies for intervention.}},
  author       = {{Johansson, Angelica and Kalliara, Eirini and Belfrage, Emma and Alling, Teodor and Pyl, Paul Theodor and Sandström Gerdtsson, Anna and Gullberg, Urban and Porwit, Anna and Drott, Kristina and Ek, Sara}},
  issn         = {{2227-9059}},
  language     = {{eng}},
  pages        = {{1--29}},
  publisher    = {{MDPI AG}},
  series       = {{Biomedicines}},
  title        = {{The Progression of Mycosis Fungoides During Treatment with Mogamulizumab: A BIO-MUSE Case Study of the Tumor and Immune Response in Peripheral Blood and Tissue}},
  url          = {{http://dx.doi.org/10.3390/biomedicines13010186}},
  doi          = {{10.3390/biomedicines13010186}},
  volume       = {{13}},
  year         = {{2025}},
}